Daneshvar Medicine

Daneshvar Medicine

The effect of high-intensity interval training and high-protein diet on the apoptotic indices of caspase-3, Bax, and Bcl-2 in the cardiac tissue of obese male rats

Document Type : Original Article

Authors
Azin Ghane 1
Hossein Abednatanzi 1
Marziyeh Saghebjoo 2
Mehdi Hedayati 3
1 Department of Professional Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran
3 3. Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10.22070/daneshmed.2022.15468.1151
Abstract
Background and Objective: Obesity is involved in the apoptosis of cardiac cells and the development of cardiovascular diseases. Exercise training and diet modification can affect apoptotic indices. The aim of the present study was to assess the effect of high-intensity interval training (HIIT) and high-protein diet (HPD) on the apoptotic indices of caspase-3, Bax, and Bcl-2 in the cardiac tissue of obese rats.
Materials and Methods: In the present experimental study, 40 obese male rats were divided into five equal groups, including HIIT (90% VO2max, 5 sessions per week, 10 weeks), HPD, HIIT+HPD, obesity control-1 (OC-1), obesity control-2 (OC-2), and eight non-obese rats were also considered in the non-obese control (NC) group. Cardiac tissue was extracted 48 hours after the last intervention session to measure the levels of caspase-3, Bax, and Bcl-2 proteins. Data were analyzed using one-way ANOVA, Kruskal-Wallis, and Mann-Whitney tests (P≤0.05).
Results: The caspase-3 and Bax levels in the HIIT+HPD group was significantly lower than in the HIIT, HPD, and OC-1 groups. On the other hand, the HIIT and HPD groups showed a significantly higher Bcl-2 level than the OC-1 and NC groups. Also, there was a significantly higher of Bcl-2 to Bax ratio in the HIIT and HIIT+HPD groups compared with the OC-1 group.
Conclusion: According to the results of the present study, a combination of HIIT and HPD improves the apoptotic status of cardiac tissue. It seems that the combination of HIIT and HPD provides the necessary adaptations to inhibit obesity-induced apoptosis in the cardiac tissue of obese rats.
Keywords
High intensity interval training
High-protein diet
Caspase-3
Cardiac tissue
Obese rats
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Daneshvar Medicine
Volume 30, Issue 1 - Serial Number 158
May and June 2022
Pages 59-71