Daneshvar Medicine

Daneshvar Medicine

The effect of diosgenin on mitochondrial membrane potential and neutrophil infiltration in methotrexate-induced testicular injury in rats

Document Type : Original Article

Authors
Fatemeh Taleahmad 1
Mohsen Khalili 2
Narges HadadZadeh 1
Ensiyeh Joneidi 3
Mehrdad Roghani 2
1 Faculty of Medicine, Shahed University, Tehran, Iran
2 Neurophysiology Research Center, Shahed University, Tehran, Iran
3 Faculty of Basic Sciences, Shahed University, Tehran, Iran
10.22070/daneshmed.2022.15053.1116
Abstract
Background and Objective: Methotrexate can induce testicular tissue damage and infertility by increasing the activity myeloperoxidase and destroying mitochondrial membrane. Diosgenin is a steroidal sapogenin with antioxidant properties. The aim of this study was to evaluate the effects of diosgenin on mitochondrial membrane potential (MMP) and neutrophil infiltration in methotrexate-induced testicular injury in the rat.
Materials and Methods: 40 rats were divided into 5 groups: control, diosgenin-treated control with a dose of 50 mg/kg, methotrexate, two groups of diosgenin-treated methotrexate at doses of 10 or 50 mg/kg. Methotrexate at a dose of 20 mg/kg was injected intraperitoneally one day after diosgenin administration. Diosgenin was administered daily for two weeks at doses of 10 or 50 mg/kg. At the end, the rats were anesthetized and the testis was removed. MPO and MMP in testicular tissue were measured. For statistical analysis, one-way ANOVA and Tukey post-test were used.
Results: The MMP of the methotrexate group showed a significant decrease compared to the control group (p=0.003). The diosgenin-treated methotrexate group at a dose of 50 mg/kg showed a significant increase as compared to the methotrexate group (p=0.008). The level of MPO in the methotrexate group showed a significant increase as compared to the control group (p=0.009). The diosgenin-treated methotrexate group at a dose of 50 mg/kg showed a significant decrease as compared to the methotrexate group (p=0.039).
Conclusion: Diosgenin reduces the harmful effects of methotrexate on MPO and MMP in testicular tissue.
Keywords
Diosgenin
Methotrexate
Neutrophilic infiltration
Mitochondrial membrane potential
Testis
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Daneshvar Medicine
Volume 30, Issue 1 - Serial Number 158
May and June 2022
Pages 27-35