Daneshvar Medicine

Daneshvar Medicine

Synthesis and optimization of effective dose of niosomal amikacin for antibacterial activity on Pseudomonas aeruginosa

Document Type : Original Article

Authors
Fardin Rahimi 1
Mahsa Bahramgur 2
Ghasem Amoabediny 2
Azam Bagheri Pebdeni 1
Bahman ebrahimi hosseinzade 2
Zeinab Amoabediny 2
1 Department of Nanobiotechnology, Research Laboratory of Shahed University, Tehran, Iran
2 Department of Biological Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
10.22070/daneshmed.2021.15391.1145
Abstract
Background and Objective: Niosomes are one of the new drug delivery systems. In this synthesis study, the properties and characteristics of nanosystems loaded amikacin for the aim of slow releasing and antibacterial activity on Pseudomonas aeruginosa were been investigated.
Materials and Methods: The synthesis nanocarriers were characterized by using DLS, zeta potential, SEM images, and FTIR analysis. The percentage of drug loading in the nanosystems were measured. After that, the release rate of the drug was checked. MIC microbiological tests were performed to determine the minimum inhibitory concentration (MIC) of Pseudomonas aeruginosa. Finally, with MTT method, the toxicity and effectiveness of the system on cell line A549 extracted from lung were evaluated.
Results: In this study, by synthesis and optimization, with the highest loading efficiency of 95.15% at the formulations and the slowest release with a drug release rate of 65.27% in 60 hours to reduce the side effects of amikacin. We were able to increase the antimicrobial activity of amikacin niosomal against Pseudomonas aeruginosa and reduce the side effects compared to the free form and have a slower and more continuous release with greater effectiveness on bacteria. Studying this process on the lung cell lines was successful and free-form of amikacin has a more toxic effect than two drug-free and drug-free niosomal systems. Numerous mathematical models, including realistic and experimental/quasi-experimental mechanistic models, have been successfully introduced to quantitatively define drug release mechanisms and play an important role in designing a drug delivery system for drug release diagrams. That reported in results
Conclusion: Achieving optimal conditions for the preparation of nanosystems carrying amikacin and investigating the effect of this system on bacterial agents is an important step in science, research, and economic efficiency.
Keywords
Nanosystem
Niosome
Amikacin
Antibacterial activity
Pseudomonas aeruginosa
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Daneshvar Medicine
Volume 29, Issue 6 - Serial Number 157
March and April 2022
Pages 86-100