Daneshvar Medicine

Daneshvar Medicine

Comparison of the effect of eight-week training in water, resistance ladder and endurance running on catalase, malondialdehyde, vaspin and insulin resistance in male rats

Document Type : Original Article

Authors
Mojtaba Sadegh Ghomi
Majid Kashef
Fereshteh Shahidi
Mojtaba Salehpour
Mehri Javadieh
Mohammad Javad Noroozi Nia
Department of Exercise Physiology, Faculty of Sport Science, Shahid Rajaee Teacher training University, Tehran, Iran
10.22070/daneshmed.2021.15238.1133
Abstract
Background and Objective: Increased lipid peroxidation and imbalance of adipokines that affect insulin sensitivity such as vaspin can be effective in the development and progression of atherosclerosis. The aim of present study was comparison and determination of effect of eight-week training in water, resistance ladder and endurance running on catalase (CAT), malondialdehyde (MDA), vaspin and insulin resistance in male rats.
Materials and Methods: A total of 41 male rats were randomly divided into 5 groups (training in water, resistance ladder, endurance running, sham and control). After two weeks of adapting to the environment and learning the exercises, the training groups exercised for eight weeks. To measure study indices, we used ELISA method and the formula of insulin resistance homeostasis model. One-way analysis of variance and Tukey's post hoc test were used to test the hypotheses.
Results: Eight-week of resistance training significantly increased catalase activity and decreased serum MDA levels more than the other two training methods. Moreover, eight weeks of endurance running and water training significantly reduced serum VASPIN and insulin resistance compared to resistance training (p<0.05).
Conclusion: It seems that resistance training to be superior to the other two training methods in reducing lipid peroxidation, while endurance running and training in water result in greater reductions in VASPIN and insulin resistance than resistance training.
Keywords
Training
Catalase
Malondialdehyde
Vaspin
Rat
  1. Ravi Kiran T, Subramanyam MVV, Asha Devi S. Swim exercise training and adaptations in the antioxidant defense system of myocardium of old rats: relationship to swim intensity and duration. Journal of Comparative Biochemistry and Physiology 2004; Part B 137.187-196
  2. Powers SK, Jackson MJ. Exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force Production. Journal of Physiology Review 2008; 88:1243-1276.
  3. Gandhi G, Gunjan G. Exercise induced genetic damage: A Review. International Journal of Human Genetic 2009; 9:69-96.
  4. Powers, S K and Jackson, M J. Exercise induced oxidative Stress: cellular mechanisms and impact on muscle force production.  Journal of Physiology Review 2008; 88:1243-1276.
  5. Brites F, Zago V, Verona J, Luz Muzzio M, Wikinski R. Schreier L. HDL capacity to inhibit LDL oxidation in well –trained triathletes . Journal of Life Science 2006; 78:3074-3081.
  6. Urso ML, Clarkson PM. Oxidative stress, exercise and antioxidant supplementation. Journal of Toxicology 2003; 189:41-54.
  7. Nayebifar SH, Afzalpour ME, Sagheb joo M, Hedayati M. Effect of aerobic and resistance training on ICAM and serum lipid profile in overweight women. Journal of Sport and Motor Bioscience 2010; 2(4):77-87.
  8. Chandan K Sen, Packer L, Osmo OP Hänninen. Hand book of oxidants and antioxidant in exercise. Journal of Amsterdam. Elsevier science 2000; 433-483.
  9. Bloomer RJ, Smith WA. Oxidative stress in response to aerobic and anaerobic power testing: influence of exercise training and carnitine supplementation. Journal of Research in Sports Medicine 2009; 17:1-16.
  10. Jackson MJ. Reactive oxygen species and redox regulation of skeletal muscle adaptations to exercise. Journal of Philosophical Transactions Royal Society 2005; B 360: 2285-2291.
  11. Huffman KM, Slentz CA, Bales CW, Houmard JA, Kraus WE. Relationships between adipose tissue and cytokine responses to randomized controlled exercise training intervention. Journal of Metabolism 2009;57(4):577-583.
  12. Roth CL, Kratz M, Ralston MM, Reinehr T. Changes in adipose–derived inflammatory cytokines and chemokines after successful lifestyle intervention in obese children. Journal of Metabolism 2011; 60(4):445-452.
  13. Heiker JT. Vaspin in obesity, insulin resistance and inflammation. Journal of Peptide Science 2014; 20(5):299-306.
  14. Jung C, Lee W, Hawang J, Seol SM, Kim YM, Lee YM, Park JY. Vaspin protect vascular endothelial cells against free fatty acid induced apoptosis through pI3K/Akt pathway. Journal of Biochemical and Biophysical Research Communications 2011; 413:264-269.
  15. Kukla M, Mazur W, Buldak R J, Żwirska-Korczala K. Potential role of leptin, adiponectin and three novel adipokines: Visfatin, Chemerin and Vaspin in chronic hepatitis. Journal of Molecular Medicine 2011; 17(11-12):1397-1410.
  16. Oberbach A, Kirsch K, Lehmann S, Schlichting N, Fasshauer M, Zarse K, et al. Serum Vaspin concentrations are decreased after exercise induced oxidative stress. Journal of Obesity Facts 2010; 3(5):328-331.
  17. Habibian M, Saghafi MR, Farzanegi P. The Effect of Regular Swimming Exercise on the Levels of Renal Matrix Mettaloproteinase-2 and Transforming Growth Factor-β1 in Rats with Diabetes. Journal of Kerman University of Medical Sciences 2016; 23(4):446-456.
  18. Lee,S et al.Viral expression of insulin-like growth factor-I enhances muscle hypertrophy in resistance-trained rats. Journal of Applied Physiology 2004; 96(3):1097-1104.
  19. Bedford, T.G., et al. Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology 1979; 47(6):1278-1283
  20. Leandro CG, Levada AD, Hirabara SM, Manhães-de-Castro R, De-Castro CB, Curi R, et al. A program of moderate physical training for Wistar rats based on maximal oxygen consumption. Journal of Strength and Conditioning Research 2007; 21(3): 751.
  21. Sasan a, Aziz Beygi R, Atashak S. Effect of two resistance protocol on lipid peroxidation and plasma total antioxidant capacity changes in health males. Journal of Sport Bioscience 2014; 6(3):245-257.
  22. Samavati Sharif MA, Vesali Akbarpou L. Comparison the effect of two kind of endurance swimming training on lipid peroxidation and muscle damages indexes in serum levels of male Wistar rats. Journal of Ilam University 2016; 19(109):80-88.
  23. Mohammadi M, Salehi I, Farajnia S. Effect of swimming exercise on oxidative stress in hypocampous of diabetic male rats. Medical Journal of Tabriz University of Medical Sciences 2008; 30(2):111-118.
  24. Nisel O, Belviranli M. Effect of exercise training on hepatic oxidative stress and antioxidant status in aged rats. Journal of Metabolic Diseases 2016; 122(4):180-185.
  25. Daud DM, Karim AAH, Mohammad N, Hamid NAA, Ngah WZW. Effect of exercise intensity on antioxidant enzymatic activities in sedentary adults. Malaysian Journal of Biochemistry and Molecular Biology 2006; 13:37-47.
  26. Soori R, Ravasi AA, Ranjbar K. Comparison of effect of endurance and resistance training on Vaspin and adiponectin serum amount in obese median men. Journal of Exercise Physiology 2012; 5(20):97-114.
  27. Kim JY, Kim ES, Jeon JY, Jekal Y. Improved insulin resistance, adiponectin and liver enzyme without change in plasma vaspin level after 12 weeks exercise training among obese male adolescents. The Korean Journal of Obesity 2011; 20(3).
  28. Mokhtari M, Daryanoosh F. The effect of 12-weeks resistance exercise on the levels of vaspin serum and blood pressure in hypertensive elderly women. Journal of Shahid Sadoughi University Medicine Science 2017; 25(1):32-41.
  29. Dabidi Roshan V O. Amoozad mahdiraji, H .Talebi Garakani, E. Effect of circuit resistance training on serum Vaspin concentration and insulin resistance in patients with type 2 diabetes. Journal of Sport Physiology and Physical Activity 2012; 9:735-744.
  30. Waston, R T. Kanzaki, M and Pessin JE. Regulated membrane trafficking of the insulin responsive glucose transporter 4 in adipocytes. Journal of Endocrinology Review 2004. 25(2):177-204
  31. Adam J. Rose, Erik A. Richter. Skeletal muscle glucose uptake during exercise: how it is regulated? Journal of American physiological Society 2005. 20:260-270
  32. Merry, TL and McConell, GK. Skeletal muscle glucose uptake during exercise: a focus on reactive oxygen species and nitric oxide signaling. International Union of Biochemistry and Molecular Biology Life. 2009 61(5):479-484
  33. Ojuka, EO. Goyaram, V and Smith JA. The role of CamKII in regulating GLUT4 expression in skeletal muscle. American Journal of Physiological Endocrinology Metabolism 2012. 303(3):E322-331.
Daneshvar Medicine
Volume 29, Issue 6 - Serial Number 157
March and April 2022
Pages 58-69