The effect of progressive endurance training on heart resistance induced by infusion ischemia in healthy male rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sports Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Animal Sciences, Faculty of Agriculture and Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran

3 Department of Physiology, School of Medicine and Molecular Medical Research Center and Applied Pharmaceutical Research, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Background and Objective: Myocardial infarction is one of the main causes of mortality in the world and endurance exercise is one of the ways to prevent it. The present study aimed to investigate the effect of endurance training on oxidative stress induced by infusion ischemia in the heart of healthy male rats.
Materials and Methods: 32 two-month-old male Wistar rats were used. Rats after one week of adaptation to the new conditions were randomly divided into four treatment groups with eight mice each: 1) health control group, 2) unhealthy control group, 3) health training group, and 4) unhealthy training group. The health training and unhealthy training groups were subjected to endurance training for eight weeks. To induce ischemia, each mouse received isoproterenol (85 mg/kg) intraperitoneally for two consecutive days. At the end of the experimental period, after anesthesia and isolation of rat and washing with ice-cold saline, then homogenization of heart tissue was performed. The activities of superoxide dismutase, glutathione peroxidase, catalase, and myeloperoxidase enzymes were measured using specific kits and supernatants to evaluate the level of antioxidant activity. ELISA method were used and data analyzed with ANOVA test at the significant level of p < 0.05.
Results: Cardiac ischemia significantly reduced the activity of antioxidant enzymes and increased lipid peroxidation compared to the control (p=0.001) and exercise groups (p=0.002). Also, eight weeks of endurance training without ischemia increased the activity of antioxidant enzymes (p=0.002) and decreased myeloperoxidase compared to the ischemia group (p=0.001).
Conclusion: Increasing endurance training by reducing oxidative stress can have significant therapeutic effects against isoproterenol-induced oxidative stress damage and possibly reduce the complications of ischemic heart failure.

Keywords

References

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Daneshvar Medicine
Volume 29, Issue 2 - Serial Number 153
July and August 2021
Pages 68-78

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