Protective effect of ghrelin on histopathological alterations in renal tissue of mice treated with cyclophosphamide

Document Type : Original Article

Authors

1 Students Research Committee, School of Paramedical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran.

2 Students Research Committee, School of Medical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran.

3 Department of Sports Physiology, Urmia University, Urmia, Iran.

4 Department of Anatomical Sciences, MaraghehUniversity of Medical Sciences Maragheh, Maragheh, Iran.

5 5. Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Abstract

Background and Objective: Cyclophosphamide is one of the common medications in chemotherapy and suppression of the immune system in organ transplantation. The clinical use of cyclophosphamide has been reduced due to its many side effects due to reproductive, hepatic, renal, and cardiac toxicity in patients and animal models. This study aimed to evaluate the effect of ghrelin against cyclophosphamide-induced damage to renal tissue.
Materials and Methods: Thirty-two (2-month old) male mice were randomly divided into 4 groups: 1) control 2) cyclophosphamide 3) cyclophosphamide + ghrelin 4) ghrelin. Cyclophosphamide once a week (100 mg/kg) and ghrelin (80 μg/kg) were injected intraperitoneally daily for 5 weeks. After 5 weeks, the kidneys were removed and after weighing, tissue alterations were examined by hematoxylin-eosin and Periodic Acid Schiff staining.
Results: Findings showed that cyclophosphamide significantly reduces body weight and kidney weight and also significantly increases tissue damage (p < 0.05). Ghrelin treatment significantly improved the above parameters (p < 0.05).
Conclusion: The results of this study showed that ghrelin can reduce the destructive effects of cyclophosphamide in renal tissue.

Keywords

References

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Daneshvar Medicine
Volume 29, Issue 2 - Serial Number 153
July and August 2021
Pages 1-11

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