Evaluation of lipoprotein lipase gene expression and activity following a session of endurance exercise in muscle tissue and plasma of rat

Document Type : Original Article

Authors

1 Department of Exercise Physiology, Sabzevar Tarbiat Moallem University, Sabzevar, Iran.

2 Department of Exercise Physiology, Sabzevar Tarbiat Moallem University, Sabzevar, Iran

3 Department of Medical Biotechnology, Tarbiat Modarres University, Tehran, Iran.

Abstract

Background and Objective:  Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and maintenance of energy homeostasis. Acute and delayed response of LPL to one session of exercise has not been well studied. The purpose of the present study was to examine the effect of one session of prolonged treadmill running on soleus muscle LPL mRNA and activity and plasma LPL activity in male Wistar rats.
 Materials and Methods: Twenty four male Wisatr rats (weight: 388 ± 31 g) were randomly divided into two groups: control group (n=12) and trained group (n=12). The exercised rats ran on treadmill for 120 minutes (18 m/min, 0 º incline). Rats were anesthetized, sacrificed, and blood and soleus muscle sample were taken 0, 2, and 24 h after exercise. Semi-quantitative RT-PCR was used to measure LPL mRNA level in soleus muscle. Data were analyzed using repeated measures ANOVA.
 Results: The results showed that muscle LPL mRNA significantly increases 2 and 24 hours after exercise (p =0.002 and p =0.004 respectively). Also, muscle LPL activity significantly increased 2 and 24 hours after exercise (p=0.03 and p=0.007 respectively). Plasma LPL activity also significantly increased 24 hours after exercise (p =0.004).
 Conclusion: The study showed that single session of prolonged exercise via increasing muscle LPL gene expression can promote triglyceride hydrolysis. Therefore, the ability of muscle for FFA oxidation increases and lipid metabolism improves.

Keywords

References

1.     Jequier E, Tappy L. Regulation of body weight in humans. Physiol Rev. 1995;  79(2): 452-472.
 2.   Arch J. Central regulation of energy balance: inputs, outputs and leptin resistance. Proc Nutr Soc. 2005; 64(1): 39-46.
 3.   Schwarts MW, Woods SC, Seely RJ, Brash GS, Baskin DG, and Leibel RL. Is the energy homeostasis system inherently biased toward weight gain? Diabetes. 2003; 52 (2): 232-238.
 4.   Woods S, and Seeley R. Adiposity signals and the control of energy homeostasis. Nutrition. 2000; 16(10): 894 –902.
 5.   Hamman A, Matthaei S. Regulation of energy balance by leptin. Exp Clin Endocrinol Diabetes. 1996; 104(4):293-300.
 6.   Hamilton M, Etienne J, Mcclure W, Pavey B, and Holloway A. Role of local contractile activity and muscle fiber type on LPL regulation during exercise. Physiol 275 (Endocrinol Metab). 1998; 38: e1016- 1022.
 7.   Tsutsumi  K. Lipoprotein lipase and atherosclerosis. Curr Vasc Pharmacol. 2003; 1(1): 11-17.
 8.   Mead JR, Iravine SA, Ramji DP. Lipoprotein lipase: structure, function, regulation and role in disease. J Mol Med. 2002; 80(12): 753-769.
 9.   Hamilton M and Bey L. Suppression of skeletal muscle lipoprotein lipase activity during physical  inactivity: a molecular reason to maintain daily low- intensity activity. J Physiology. 2003; 551(2):673-682.
 10. Kiens B, Roepstroff C, Glatz JFC, Bonen A, Schjerling P, Knudsen J, and Nielsen JN. Lipid binding protein and lipoprotein lipase activity in human skeletal muscle: influence of physical activity and gender. J Appl Physiol. 2004; 97:1209-1218.
 11. Goldberg IJ, Eckel RH, Abumard NA. Regulation of fatty acid uptake into tissue: lipoprotein lipase and CD36-mediated pathways. J lipid Res. 2009; 50: S86- S90.
 12. Zurlo F, Larson K, Bogardus C, and Ravussin E. Skeletal muscle metabolism is a major determinant of resting energy expenditure. J Clin Invest. 1990; 86(5): 1423–1427.
 13. Atanassova P, Delchev S, Georgieva K, Koeva Y. Lipoprotein lipase enzyme histochemical activity in subcutaneous adipose tissue and skeletal muscle of the rat after submaximal exercise training. J. Conference of Biol. 2005; 263-268.
 14. Bergeron J. Race differences in the response of postheparin plasma lipoprotein lipase and hepatic lipase activities to endurance exercise training in men Results from the HERITAGE Family Study.
Atherosclerosis. 2001;159( 2): 399-406
 15. Herd SL, Hardman AE, Boobis LH, Cairns CJ. The effect of 13 weeks of running training followed by 9 d of detraining on postprandial lipaemia. Br J Nutr. 1998 ;80(1):57-66.
 16. Seip R, Mair K, Cole T, and Semenkovich C. Induction of human skeletal muscle lipoprotein lipase gene expression by short-term exercise is transient. J Physiol. 1997; 272: 255- 261.
 17. Seip RL, Poulos TJA, Kovich CFS. Exercise induces human lipoprotein lipase gene expression in skeletal muscle but not adipose tissue. Am J Physiol. 1995 Feb;268(2 Pt 1):E229-36
 18. Ladu M، Kapsas H and Palmer W. Regulation of lipoprotein lipase in muscle and adipose tissue during exercise. J Appl Physiol. 1991; 71(2): 404- 409.
 19. Ong J, Simsolo R, Saghizadeh M, Goers J, and Kern P. Effects of exercise training and feeding on lipoprotein lipase gene expression in adipose tissue, heart, and skeletal muscle of the rat. Metabolism. 1995;44(12):1596-605.
 20. Paulin A, Lalonde J, Deshaies Y. Beta-adrenergic blockade and lipoprotein lipase activity in rat tissues after acute exercise. Am J Physiol. 1991;261(4 Pt 2):R891-7.
 21. Magnoni L, and Weber J-M. Endurance swimming activates trout lipoprotein lipase: plasma lipids as a fuel for muscle. J Experim Biol. 2007, 210: 4016-4023
 22. Kern PA. Potential role of TNFα and lipoprotein lipase as candidate genes for obesity. J Nutr. 1997, 127: 1971S-1922S.
 23. Perreault L, Lavely JM, Kittelson JM, Horton TJ. Gender differences in lipoprotein lipase activity after acute exercise. Obes Res. 2004; 12(2): 241- 249.
 24. Donahoo WT, Jensen DR, Shepard TY, and Eckel RH. Seasonal variation in lipoprotein lipase and plasma lipids in physically active, normal weight humans. J Clin Endocrinol Metab 2000, 85: 3065-3068
 25. Oscai LB, Tsika RW, Essig DA. Exercise training has a heparin-like effect on lipoprotein lipase activity in muscle. Can J Physiol Pharmacol. 1992;70(6):905-9.
 26. Friedman JM, Hallas JL. Leptin and the regulation of body weight in mammals. Nature. 1998; 395: 763-770.
 27. Lin X, Chavez M, Bruch R, Kilroy G, Simmons L, Lin L, et al. The effects of a high fat diet on leptin mRNA, serum leptin and the response to leptin are not altered in a rat strain susceptible to high fat diet-induced obesity. J Nutr. 1998; 128: 1606–1613.
 28. Pataly M, Lofgren I, Freake H, Koo S, and Fernandez M. The lowering of plasma lipids following a weight reduction program is related to increased expression of the LDL receptor and lipoprotein lipase. J Nutr. 2005; 135: 735–739.
Daneshvar Medicine
Volume 19, Issue 2 - Serial Number 93
May and June 2011
Pages 61-70

Files

Share

How to cite

Statistics