Daneshvar Medicine

Daneshvar Medicine

The effect of 8-week of high intensity interval training on PGC-1α and ERRα gene expression in subcutaneous adipose tissue of male Wistar rats

Document Type : Original Article

Authors
Shadi Fayyazi
Mohammadreza Asad
Saeed Naghibi
Mohammad Shariatzade
Faculty of Physical Education, Department of Sport Physiology, PNU, Karaj, Iran
10.22070/daneshmed.2020.2975
Abstract
Background and Objective: PGC-1α and ERRα work together to transcribe genes involved in energy metabolism and play an important role in the expression and control of metabolic genes. The role of exercise in the expression of these two genes in adipose tissue is less known. Therefore, this study aimed to investigate the effects of high intensity interval training (HIIT) on the expression of PGC-1α and ERRα genes in the subcutaneous adipose tissue of male Wistar rats.
Materials and Methods: In the experimental study, 16 male rats were divided into two equal groups of HIIT and control. HIIT group participated in training programs for 8 weeks. The control group did not participate in the training program. The HIIT protocol consisted of 4 stages of running in 4 minutes with intensity of 90 to 100% VO2max and 4 stages of running in 3 minutes in 50 to 60% of VO2max. 24 hours after the last training session, the subcutaneous adipose tissue was removed. The RT-PCR method was used to measure gene expression. Data analysis was performed with independent t-test and Mann-Whitney U at the significance level of P < 0.05.
Results: In the HIIT protocol, a significant increase was observed in the expression of both PGC-1α and ERRα genes in comparison with the control group (P < 0.001).
Conclusion: The results showed that HIIT training is very effective in expressing the genes involved in energy metabolism. Therefore, it is recommended that this training method be given more attention.
Keywords
Subcutaneous adipose tissue
HIIT
PGC-1α
ERRα
  1. Frigolet ME, Gutiérrez-Aguilar R. The colors of adipose tissue. Gaceta Medica de Mexico 2020; 156(2):142-149.
  2.   Ultimo S, Zauli G, Martelli AM, Vitale M, McCubrey JA, Capitani S, et al. Cardiovascular disease-related miRNAs expression: potential role as biomarkers and effects of training exercise. Oncotarget 2018; 9(24):17238-17254.
  3.   Shalaby RE, Iram S, Oropeza CE, McLachlan A. Peroxisome proliferator-activated receptor γ coactivator family members competitively regulate hepatitis b virus biosynthesis. Virology 2019; 526:214-221.
  4. McKie GL, Wright DC. Biochemical adaptations in white adipose tissue following aerobic exercise: from mitochondrial biogenesis to browning. Biochemical Journal 2020; 477(6):1061-1081.
  5. Cantó C, Auwerx J. PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology 2009;20(2):98-105.
  6. Son YO, Chun JS. Estrogen-related receptor γ is a novel catabolic regulator of osteoarthritis pathogenesis. BMB Reports 2018;51(4):165-166.
  7. Deblois G, Giguère V. Functional and physiological genomics of estrogen-related receptors (ERRs) in health and disease. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 2011;1812(8):1032-1040.
  8.   Angueira AR, Shapira SN, Ishibashi J, Sampat S, et al. Early B cell factor activity controls developmental and adaptive thermogenic gene programming in adipocytes. Cell Reports 2020;30(9):2869-2878.
  9.   Manio MC, Matsumura S, Inoue K. Low-fat diet, and medium-fat diets containing coconut oil and soybean oil exert different metabolic effects in untrained and treadmill-trained mice. Journal of the International Society of Sports Nutrition 2018;15(1):29.
  10. Shapira SN, Seale P. Transcriptional control of brown and beige fat development and function. Obesity 2019;27(1):13-21.
  11. Arany Z, Foo SY, Ma Y, et al. HIF-independent regulation of VEGF and angiogenesis by the transcriptional coactivator PGC-1alpha. Nature 2008;451(7181):1008‐1012.
  12. Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomolecules 2015;5(2):356-377.
  13. Yan M, Audet-Walsh É, Manteghi S, Dufour CR, Walker B, Baba M, St-Pierre J, Giguère V, Pause A. Chronic AMPK activation via loss of FLCN induces functional beige adipose tissue through PGC-1α/ERRα. Genes & Development 2016;30(9):1034-46.
  14. Saito K, Cui H. Emerging roles of estrogen-related receptors in the brain: potential interactions with estrogen signaling. International Journal of Molecular Sciences 2018;19(4):1091.
  15. Kleiner S, Mepani RJ, Laznik D, Ye L, Jurczak MJ, Jornayvaz FR, et al. Development of insulin resistance in mice lacking PGC-1α in adipose tissues. Proceedings of the national academy of sciences 2012;109(24):9635-9640.
  16. Semple R, Crowley V, Sewter C, Laudes M, Christodoulides C, Considine R, et al. Expression of the thermogenic nuclear hormone receptor coactivator PGC-1α is reduced in the adipose tissue of morbidly obese subjects. International Journal of Obesity 2004;28(1):176-179
  17. Supruniuk E, Mikłosz A, Chabowski A. The implication of PGC-1α on fatty acid transport across plasma and mitochondrial membranes in the insulin sensitive tissues. Frontiers in Physiology 2017;8(923).
  18. Austin S, Klimcakova E, St-Pierre J. Impact of PGC-1α on the topology and rate of superoxide production by the mitochondrial electron transport chain. Free Radical Biology and Medicine 2011;51(12):2243-8.
  19. Sharp LZ, Shinoda K, Ohno H, Scheel DW, Tomoda E, Ruiz L, et al. Human BAT possesses molecular signatures that resemble beige/brite cells. PloS One Journal 2012;7(11).
  20. Komen J, Thorburn D. Turn up the power–pharmacological activation of mitochondrial biogenesis in mouse models. British Journal of Pharmacology 2014;171(8):1818-36.
  21. Rocha-Rodrigues S, Rodríguez A, Gouveia AM, Gonçalves IO, Becerril S, Ramírez B, et al. Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet. Life Sciences 2016;165:100-108.
  22. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation 2007;14(6):753-760.
  23. Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, et al. The effects of acute and chronic exercise on PGC-1alpha, irisin and browning of subcutaneous adipose tissue in humans. The FEBS Journal 2014;281(3):739-749.
  24. khalafi M, Mohebbi H, Karimi P, Faridnia M, Tabari E, The effect of high intensity interval training and moderate intensity continuous training on mitochondrial content and PGC-1α of subcutaneous adipose tissue in male rats with high fat diet induced obesity. Sport Biosciences 2018;38:297-315.
  25. Zhou J, Poudel A, Chandramani-Shivalingappa P, Xu B, Welchko R, Li L. Liraglutide induces beige fat development and promotes mitochondrial function in diet induced obesity mice partially through AMPK-SIRT-1-PGC1-α cell signaling pathway. Endocrine 2019;64(2):271-283.
  26. Chandrasekaran K, Anjaneyulu M, Choi J, Kumar P, Salimian M, Ho CY, Russell JW. Role of mitochondria in diabetic peripheral neuropathy: Influencing the NAD+-dependent SIRT1–PGC-1α–TFAM pathway. International Review of Neurobiology 2019;145:177-209.
  27. Deblois G, Giguere V. Functional and physiological genomics of estrogen-related receptors (ERRs) in health and disease. Biochimica Biophysica Acta 2017; 1812(8):1032-40.
  28. Cartoni R, Leger B, Hock MB, Praz M, Crettenand A, Pich S, et al. Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise. The Journal of Physiology 2018; 567(1):349-358.
  29. Ringholm S, Knudsen JG, Leick L, Lundgaard A, Nielsen MM, Pilegaard H. PGC-1α is required for exercise-and exercise training-induced UCP1 up-regulation in mouse white adipose tissue. PloS One 2013;8(5):e6412
  30. Brenner DR, Ruan Y, Adams SC, Courneya KS, Friedenreich CM. The impact of exercise on growth factors (VEGF and FGF2): results from a 12-month randomized intervention trial. European Review of Aging and Physical Activity 2019;16(1):8.
Daneshvar Medicine
Volume 28, Issue 4 - Serial Number 149
September and October 2020
Pages 23-34