Evaluation of niosomal nano-carriers capabilities on toxicity preservation and delivery of pomegranate peel extract in cell culture conditions (MCF-7 cell line of breast cancer)



Background and Objective: Use of nano-carriers that contain plant cytotoxic compounds instead of common chemotherapy drugs can reduce many of the challenges in front of this method of cancer therapy. In this study, surfactant nano-systems containing pomegranate peel extract were designed to evaluate its cytotoxic effects on MCF-7 cell line (breast cancer) and investigated its physicochemical properties.
Materials and Methods: Niosomal vesicles were prepared using span60, cholesterol and polyethylene glycol by thin-film method and the pomegranate peel extract were loaded into the niosomes. Physicochemical characteristics were evaluated using Zeta Sizer, FTIR, SEM, and extract release amount was calculated at 37°C and 42°C. The toxicity of the nano-carrier containing extract was measured on the MCF-7 cell line of breast cancer using MTT assay.
Results: The results of this study indicate that the niosomes-containing extract has the encapsulation efficiency, size, and surface charge as 61.28%, 14.36 nm, and 40.9 mV, respectively. The study of its releasing also shows that the niosomal nano-carrier has controlled release at 37°C and 42°C. The study of FTIR confirms that there is no interaction between peel extract and nano-carrier. Also, SEM shows the spherical morphology of the nano-carriers. MTT results demonstrate that the toxic effects of niosomes-contained pomegranate peel extract on MCF-7 cell line of breast cancer is higher than peel extract alone.
Conclusion: According to the results of this investigation, it is indicated that the carrier niosomal nano vesicles can be a suitable carrier for delivery of pomegranate peel extract in conditions of cancerous cells.


1. Onsory KH, Ranapour S. Breast Cancer in Women and the Role of Environmental Factors in Creating. New Cellular and Molecular Biotechnology Journal 2011; 1(4): 59-70 2. Parvin Yavari, Mosavizadeh M, Sadrolhefaz IB, Khodabakhshi R, Madani H, Mehrabi Y. Reproductive Characteristics and the Risk of Breast Cancer: A Case-Control Study. Iranian Journal of Epidemiology 2006; 1 (3 and 4):11-19 3. Anand P , Sundaram C , Jhurani S , Kunnumakkara AB , Aggarwal BB. Cancer Letters 2008, 267(1):133-164 4. Rahimzadeh M, Sadeghizadeh M, Najafi F, Arab SS, Mobasheri H. Study of Loading , Cytotoxicity, Uptake, and Release of Curcumin from a Novel Gemini Surfactant Nanocarrier. Pathobiology Research 2016;19(1):13–27. 5. Mirjalili S. A Review on Biochemical Constituents and Medicinal Properties of Pomegranate (Punica granatum L.). Journal of Medicinal Plants 2015; 4 (56):1-22. 6. Hasanpour Fard M, Hassanzadeh Taheri M.M, Hosseini M, Ahani A, Ravanbakhsh N, Rabiei N, Ghoreishi S.A. Evaluation of anti-obesity and hypolipidemic effects of aqueous and ethanolic extracts of Pomegranate Peel in male Wistar Rats. Journal of Birjand University of Medical Sciences 2015; 22 (1): 39-47 7. Tarkhasi A, Zakipour Rahimabadi E, Alizadeh doughikollaee E, Elahi M.Y. Effect of edible coating containing pomegranate (Punica granatum) peel extract on the quality and shelf life of silver carp (Hypophthalmichthys molitrix) fillet during refrigerated storage. Journal of Fisheries Science and Technology 2016; 5(2): 17-26. 8. Selahvarzi Y, Tehranifar A, Jahanbakhas V. Association of antioxidant and antifungal activity of different parts of pomegranate (Punica granatum L.) with its phenolic content. Iranian Journal of Medicinal Plants and Aromatic Plants 2011; 27(1): 47-56 9. Asghari J, Mahmoudi-Alami M, Mzaheri-Tehrani M. Study of steroidal saponins in rhizome of Ruscus echolatosus L. in northern Iran (Soydokou city). Quarterly Journal of Ecophythemistry of Medicinal Plants 2012; 3(2): 28-38. 10. Chandu V, Arunachalam A,Jeganath S, Yamini K, Tharangini K, Chaitanya G. Niosomes: A Novel Drug Delivery System. International Journal of Novel Trends in Pharmaceutical Sciences 2012; 2(1): 25-31. 11. Kumar A, Pal J, Jaiswal A, Singh V. Review on niosomes as novel drug delivery system. International Research Journal of Pharmacy 2011; 2(5): 61–65. 12. R. Z. Mujoriya RZ, Dhamandeb K, Bodla RB. Niosomal Drug Delivery System–A Review. International Journal of Applied Pharmaceutics 2011; 3(1): 7–10. 13. Jain S, Jain V, Mahajan SC, Lipid Based Vesicular Drug Delivery Systems. Advances in Pharmaceutics 2014; 2014(1): 1–12. 14. Majdizadeh M, Rezaei Zarchi S, Movahedpour AA, Shahi Malmir H, Sasani E, Haghiralsadat BF. A new strategy in improving therapeutic indexes of medicinal herbs: preparation and characterization of nano-liposomes containing Mentha piperita essential oil. Journal of Shahid Sdoughi University of Medical Science 2018;25(10):853–64. 15. Haghiralsadat F, Azhdari M, Kalantar SM, Nadernezhad S, Teymorizadeh K, Yazdani M, et al. Strategy of Improvements in the rapeutic index of medicinal herbs of Iranianin digenous: Synthesis and characterization of phospholipid lipid-based vesicles in corporated Trachyspermum copticum. Journal of Shahid Sdoughi University of Medical Science 2016;24(6):468–78. 16. Detoni CB, Hohlemweger SVA, Sampaio C, Barros TF. Essential oil from Zanthoxylum tingoassuiba loaded into multilamellar liposomes useful as antimicrobial agents. Journal of Microencapsulation 2009;26(8):684–91. 17. Bei D, Meng J, Youan B-BC. Engineering nanomedicines for improved melanoma therapy: progress and promises. Nanomedicine 2010;5(9):1385-99. 18. Saffari H, Saffari M, Arj A, Haghir-Ebrahim-Abadi A. Comparing the antimicrobial properties of pomegranate seed and peel extract with common antibiotics used on helicobacter pylori isolated from biopsies of patients referring to Kashan Shahid Beheshti hospital. Feyz 2012; 16(5): 426-32 19. Anbarasan B, Rekha S, Elango K, Shriya B, Ramaprabhu S. Optimization of the formulation and in-vitro evaluation of Capecitabine Niosomes for the treatment of Colon Cancer. International Journal of Pharmaceutical Sciences and Research 2013;4(4):1504. 20. Celia C, Trapasso E, Locatelli M, Navarra M, Ventura CA, Wolfram J, Carafa M, Morittu VM, Britti D, Di Marzio L PD. Anticancer activity of liposomal bergamot essential oil (BEO) on human neuroblastoma cells. Colloids and Surfaces: B Biointerfaces 2013;112:548–53. 21. Tao F, Hill L, Peng Y, Gomes C. Synthesis and characterization of β-cyclodextrin inclusion complexes of thymol and thyme oil for antimicrobial delivery applications. LWT-Food Science and Technology 2014;59(1):247–55. 22. Haghjoo S, Ghanbarzadeh B, Hamishekar H, Asnaashari S, Dehghannia J. Evaluation of colloidal and antioxidant properties of nano liposomes containing nettle extract. Innovations in Food Technology 2015;2(7):11–23. 23. Sahra Bashiri, Ghanbarzadeh B, Hamishekar H, Dehghannya J. Beta-Carotene loaded nanoliposome: effects of gama –oryzanol on particle size stability and encapsulation. Journal of Research and Innovation in Food Science and Technology 2015;4(4):365–82. 24. Ebrahimi Khousfi M, Khosravi darani K, Hoseini H, Arabi S, Kamali Fonoud R, Kouhi Kamali P. Production of nanoliposomes containing essential oil of Boiss Zatariamultiflora by response surface method. Nano Scale 2014;1(2):119–28. 25. Cui H, Zhao C, Lin L. The specific antibacterial activity of liposome-encapsulated Clove oil and its application in tofu. Food Control 2015;56(1):128–34. 26. Gharenaghadeh S, Samadlouie HR, Sowti M, Hamisekar H, Mokaram RR. Evaluation of the antimicrobial and antioxidant properties of Salvia essential oil nano liposome (Salvia multicaulis). Journal of Food Science and Technology 2017;14(62):271–82. 27. Naderinezhad S, Haghiralsadat BF, Amouabedini G, Naderinezhad A, Esmaeili Z, Akbaezadeh A. Synthesis of biodegradable and self-assembled anionic nano –carrier: Novel approach for improvement of Curcumin- delivery to bone tumors cells & Mathematical modeling of drug-release kinetic. New Cellular and Molecular Biotechnology Journal 2017;7(27):77–84.