The response of irisin serum levels to eight weeks aerobic training with moderate intensity in obese male Wistar rats

Authors

Abstract

Background and Objective: Irisin was identified as a myokine secreted by exercise which plays an important role in energy metabolism and regulation of metabolic diseases such as obesity. The aim of this study was to investigate the effects of eight weeks aerobic training on serum levels of irisin in male obese Wistar rats.
 
Materials and Methods: In this experimental study, 16 adult rats (weight: 250 to 300 g, BMI >30 g/cm2) were divided into two groups: aerobic training with 70 to 75% Vo2max (moderate intensity) and control group. Aerobic training included: eight weeks walking on treadmill (5 sessions/per week for 60 min per session). After the training period, the level of irisin was measured. Statistical techniques for data analysis were paired and independent sample t-test to determine the intra- and inter-group comparisons between groups and the level of significance was considered at p<0.05.
 
Results: The serum levels of irisin in the aerobic training group with moderate intensity significantly increased as compared to the control group (t=4.18, p=0.001). In addition, weight in aerobic training significantly reduced.
 
Conclusion: According to the results, eight weeks of aerobic training lead to a significant increase in serum levels of irisin. Moreover, it seems that aerobic training with moderate intensity has led to an increase in theremogenesis, weight loss, and energy expenditure.
 

Keywords


1. Fakhouri T, Ogden CL, Carroll MD, Kit BK, Flegal KM: Prevalence of obesity among older adults in the United States, 2007–2010. NCHS Data Brief 2012;106:1-8. 2. Nguyen DM, El-Serag HB: The epidemiology of obesity. Gastroenterology Clinics of North America 2010;39:1-7. 3. De Onis M, Blössner M, Borghi E: Global prevalence and trends of overweight and obesity among preschool children. The American Journal of Clinical Nutrition 2010;92:1257-64. 4. Devarshi PP, Jangale NM, Ghule AE, Bodhankar SL, Harsulkar AM: Beneficial effects of flaxseed oil and fish oil diet are through modulation of different hepatic genes involved in lipid metabolism in streptozotocin–nicotinamide induced diabetic rats. Genes & Nutrition 2013;8:329-42. 5. Sigal RJ, Kenny GP, Wasserman DH, Castaneda-Sceppa C, White RD: Physical activity/exercise and type 2 diabetes. Diabetes Care 2006;29:1433-8. 6. 6. Colberg SR, Sigal RJ, Fernhall B, Regensteiner J: Exercise and Type 2 Diabetes: The American College of Sports Medicine and the American Diabetes A. Diabetes Care 2010;33:12. 7. Egan B, Zierath JR: Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metabolism 2013;17:162-84. 8. Pedersen BK, Febbraio MA: Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nature Reviews Endocrinology 2012;8:457-65. 9. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al.: A PGC1-a dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012;481:463-468. 10. Haas B, Schlinkert P, Mayer P, Eckstein N: Targeting adipose tissue. Diabetology & Metabolic Syndrome 2012;4:43. 11. Lee P, Linderman JD, Smith S, Brychta RJ, Wang J, Idelson C, et al.: Irisin and FGF21 are cold-induced endocrine activators of brown fat function in humans. Cell Metabolism 2014;19:302-9. 12. Timmons JA, Baar K, Davidsen PK, Atherton PJ: Is irisin a human exercise gene? Nature 2012;488:E9-E10. 13. Garekani ET, Mohebbi H, Kraemer RR, Fathi R: Exercise training intensity/volume affects plasma and tissue adiponectin concentrations in the male rat. Peptides 2011;32:1008-12. 14. Jashni HK, Mohebbi H, Delpasand A, Jahromy HK: Caloric restriction and exercise training, combined, not solely improve total plasma adiponectin and glucose homeostasis in streptozocin-induced diabetic rats. Sport Sciences for Health 2010; 1-6. 15. Shepherd R, Gollnick P: Oxygen uptake of rats at different work intensities. Pfluegers Archiv 1976;362:219-22. 16. Lee S, Farrar RP: Resistance training induces muscle-specific changes in muscle mass and function in rat. Journal of Exercise Physiology Online 2003;6:80-7.Huh JY, Siopi A, Mougios V, Park KH, Mantzoros CS: Irisin in response to exercise in humans with and without metabolic syndrome. The Journal of Clinical Endocrinology & Metabolism 2015;100:E453-E7. 17. Winn NC, Grunewald ZI, Liu Y, Heden TD, Nyhoff LM, Kanaley JA: Plasma irisin modestly increases during moderate and high-intensity afternoon exercise in obese females. PLoS One 2017;12:e0170690. 18. Daskalopoulou SS, Cooke AB, Gomez Y-H, Mutter AF, Filippaios A, Mesfum ET, et al.: Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects. European Journal of Endocrinology 2014;171:343-52. 19. Kim H-J, Lee H-J, So B, Son JS, Yoon D, Song W: Effect of aerobic training and resistance training on circulating irisin level and their association with change of body composition in overweight/obese adults: a pilot study. Physiological Research 2016;65:271. 20. Huh JY, Panagiotou G, Mougios V, Brinkoetter M, Vamvini MT, Schneider BE, et al.: FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism 2012;61:1725-38. 21. Roca-Rivada A, Castelao C, Senin LL, Landrove MO, Baltar J, Crujeiras AB, et al.: FNDC5/irisin is not only a myokine but also an adipokine. PLoS One 2013;8:e60563. 22. Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, et al.: The effects of acute and chronic exercise on PGC‐1α, irisin and browning of subcutaneous adipose tissue in humans. FEBS Journal 2014;281:739-49. 23. Lecker SH, Zavin A, Cao P, Arena R, Allsup K, Daniels KM, et al.: Expression of the irisin precursor FNDC5 in skeletal muscle correlates with aerobic exercise performance in patients with heart failure. Circulation: Heart Failure 2012;5:812-8. 24. Suwa M, Nakano H, Radak Z, Kumagai S: Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor γ coactivator-1α protein expressions in rat skeletal muscle. Metabolism 2008;57:986-98. 25. Hargreaves M, McKenna MJ, Jenkins DG, Warmington SA, Li JL, Snow RJ, et al.: Muscle metabolites and performance during high-intensity, intermittent exercise. Journal of Applied Physiology 1998;84:1687-91. 26. Knutti D, Kressler D, Kralli A: Regulation of the transcriptional coactivator PGC-1 via MAPK-sensitive interaction with a repressor. Proceedings of the National Academy of Sciences 2001;98:9713-8. 27. Jäger S, Handschin C, Pierre JS, Spiegelman BM: AMP-activated protein kinase (AMPK) action in skeletal muscle via direct phosphorylation of PGC-1α. Proceedings of the National Academy of Sciences 2007; 104(29):12017-12022. 28. Chen Z-P, Stephens TJ, Murthy S, Canny BJ, Hargreaves M, Witters LA, et al.: Effect of exercise intensity on skeletal muscle AMPK signaling in humans. Diabetes 2003;52:2205-12. 29. Winder W, Hardie D: AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes. American Journal of Physiology-Endocrinology and Metabolism 1999;277:E1-E10. 30. Winder W: Intramuscular mechanisms regulating fatty acid oxidation during exercise. Advances in Experimental Medicine and Biology 1998;441:239-48. 31. Hadie D, Carling D: The AMP-activated protein kinase-fuel gauge of the mammalial cell. European Journal of Biochemistry 1997;246:259-73. 32. Raney MA, Yee AJ, Todd MK, Turcotte LP: AMPK activation is not critical in the regulation of muscle FA uptake and oxidation during low-intensity muscle contraction. American Journal of Physiology-Endocrinology and Metabolism 2005;288:E592-E8. 33. Raney MA, Turcotte LP: Regulation of contraction-induced FA uptake and oxidation by AMPK and ERK1/2 is intensity dependent in rodent muscle. American Journal of Physiology-Endocrinology and Metabolism 2006;291:E1220-E7.