Comparison of nitric oxide secretion by human adipose tissue-derived mesenchymal stem cells in healthy pregnant women and those with preeclampsia

 Background and Objective: Preeclampsia is one of the
most common
complications
during pregnancy
that occurs after 20th weeks of pregnancy in women with normal blood
pressure. The pathophysiology of this disease is unknown. Due
to changes in serum level of nitric oxide in women with preeclampsia and also
the important role of mesenchymal stem cells in the secretion of nitric oxide
as an immunoregulator, in this study, we aimed to evaluate the level of nitric
oxide in secretions of adipose tissue-derived mesenchymal stem cells
in women with normal pregnancy and patients with preeclampsia.   Materials and Methods: Subcutaneous adipose tissue
of 10
preeclamptic patients and 10 healthy pregnant women was collected during cesarean operation.
After
isolation and
proliferation of mesenchymal
stem cells, capablity
of their differentiation
and their immunophenotyping charactetistics were assessed. Then,
their release
of nitric oxide
was evaluated
using Griess
method.   Results: Stem cells isolated from adipose
tissue in both groups differentiated into osteocyte and adipocytes. Flow-cytometric
analysis
showed the
expression of
the markers
CD90, CD73 CD44
and
CD105
and lack of
expression of the markers
CD-14, CD34,
CD45 and
HLA-DR
in both
groups.
No significant
change
was observed for
the level
of nitric
oxide secretion in
both groups.   Conclusion: The present results
suggest that nitric oxide secreted by mesenchymal stem cells do not have a
significant contribution in variation of serum level of this factor and
possibly do not play a role in the pathology of preeclampsia. However, such
study with a higher sample size is suggested.       Results: Stem cells isolated from adipose
tissue in both groups differentiated into osteocyte and adipocytes. Flow-cytometric
analysis
showed the
expression of
the markers
CD90, CD73 CD44
and
CD105
and lack of
expression of the markers
CD-14, CD34,
CD45 and
HLA-DR
in both
groups.
No significant
change
was observed for
the level
of nitric
oxide secretion in
both groups.   Conclusion: The present results
suggest that nitric oxide secreted by mesenchymal stem cells do not have a
significant contribution in variation of serum level of this factor and
possibly do not play a role in the pathology of preeclampsia. However, such
study with a higher sample size is suggested.