Daneshvar Medicine

Daneshvar Medicine

Immunocytochemical evaluation of expression of neuroepithelial, neural and GABAergic-like neuron markers in transdifferentiated BMSCs using appropriate in vitro inducers

Authors
Shahram Darabi
Taki Tiraihi
Alireza Delshad
Majid Sadeghizadeh
Abstract
 Background and Objective: Bone
marrow stromal cells (BMSCs) have been
shown to be a feasible option for cell therapy. There is increasing evidence that BMSCs have the potential of differentiation into GABAergic like
neuron cells (GLNCs) in vitro under appropriate induction protocols. GABAergic
dysfunction is implicated in several neurological disorders. The present study
provides evidence for an in vitro potential of BMSCs to transdifferentiate
into GLNCs.  Materials and Methods: BMSCs were
collected from long bones of adult Sprague–Dawley rats, following three passages of cell culture, BMSCs
were preinduced using 1 mM β-mercaptoethanol (βME) and 10 µM retinoic acid
(RA), they were then induced by creatine 5 mM in different days as an inducer.
In preinduction and induction stages, the effects of these inducers on
differentiation of BMSCs were evaluated by immunohistochemistry and RT-PCR.  Results: Our results indicated that
after induction stage, a high percentage of GLNCs is expressed. According to
GABAergic markers (GABA antibody), 5 mM creatine and 10 µM RA after 4 days had
a prominent role on differentiation of BMSCs to GLNCs. Conclusion: βME, RA and creatine induce
differentiation of BMSCs into GLNCs. 
Keywords
Bone marrow stromal cell
GABAergi neuron-like cells
Retinoic acid
creatine
Daneshvar Medicine
Volume 20, Issue 4 - Serial Number 101
September and October 2012
Pages 43-52