Background & Objective : Bone marrow stromal cells (BMSCs) are a kind of stem cells with high pluripotency. The BMSCs can differentiate into mesodermal and non mesodermal cells. Because of availability of them, they are a suitable source of adult stem cells for cell therapy. Some inducers were used to differentiate stem cells into neural phenotype, such: retinoic acid, dimethyl sulfoxide, deprenil & et al are cytotoxic compound. Lithium is a mood stabilizer drug that has supportive effect on neuronal cells. Our goal in this study is use of lithium chloride as a safe inducer for induction of BMSCs into neuronal phenotype. Materials & Methods: The BMSCs were isolated from bone marrow of female rat’s femurs and tibias by aspiration and culturing. After 4 passages they were induced by lithium chloride (0.5mM) for 24 hours. Then they evaluated with microscopic and immunocytochemistry methods. In immunocytochemistry, anti-neurofilament 160, 68 and 200 KD and anti-synaptophysin antibody were used to characterize the neuronal phenotype. Results: The results indicated that lithium chloride (0.5mM) can induced the BMSCs into neuronal phenotype after 24 hours. The percentage of fibronectin immunoreactive cells before induction was 95% and the percentage of neural markers immunoreactive cells after induction were 89.6% for neurofilament (NF) 68, 92% for NF-160, 93.2% for NF-200 and 70.2% for synaptophysin.
alizadeh, A., tarihi, T., & dashtnavard, H. (2009). The Influence of Lithium Chloride on Induction of Bone Marrow Stromal Cells into Neuronal Phenotype. Daneshvar Medicine, 15(6), 51-56.
MLA
akram alizadeh; taghi tarihi; hossein dashtnavard. "The Influence of Lithium Chloride on Induction of Bone Marrow Stromal Cells into Neuronal Phenotype". Daneshvar Medicine, 15, 6, 2009, 51-56.
HARVARD
alizadeh, A., tarihi, T., dashtnavard, H. (2009). 'The Influence of Lithium Chloride on Induction of Bone Marrow Stromal Cells into Neuronal Phenotype', Daneshvar Medicine, 15(6), pp. 51-56.
VANCOUVER
alizadeh, A., tarihi, T., dashtnavard, H. The Influence of Lithium Chloride on Induction of Bone Marrow Stromal Cells into Neuronal Phenotype. Daneshvar Medicine, 2009; 15(6): 51-56.
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