Authors

Abstract

Background and Objective: Alopecia Areata (AA) is characterized by an autoimmune hair loss usually, but not limited to, the scalp, affecting approximately 1-2% of the general population. Although the etiology and pathogenesis of this disease is still unknown, it is known that TNFα-308G/A polymorphism can influence the development of AA. The aim of this study was to investigate TNF-α(rs1800629) in patients with Alopecia Areata.
 
Materials and Methods: This study included 30 AA patients and 15 healthy subjects. Genomic DNA was isolated using DNG plus method and DNA was amplified by PCR-RFLP to detect TNFα-308G/A polymorphism. Furthermore, association of this TNFα-308G/A with demographic factors was assessed.
 
Results: PCR-RFLP results showed that GG was the most frequent genotype in both patient and control groups (P= 0.816). GA genotype was detected in 30% and 20% of patients and controls, respectively (P= 0.477). Genotype AA was detected in 6.7% of controls.
 
Conclusion: The results of TNFα-308G/A polymorphism showed no significant difference with respect to AA disease and demographic factors (p>0.05).
 

Keywords

References

1. Alkhalifah A, Alsantali A, Wang E, McElwee KJ, Shapiro J. Alopecia areata update: part I. Clinical picture, histopathology, and pathogenesis. The Journal of American Academy of Dermatology 2010 62:177–188. 2. Thomas EA, Kadyan RS. Alopecia areata and autoimmunity: a clinical study. Indian Journal of Dermatology 2008;53:70–74. 3. Cristina CS, Mauricio SS, Armando LR, Celia SD, Clara RI, Rocío OL, Oliverio W, Jorge OC. Tumor necrosis factor alpha promoter-308G/A polymorphism in Mexican patients with patchy alopecia areata. International Journal of Dermatology 2012;51:571–575. 4. Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proceedings of the National Academy of Sciences of the United States of America. 1975;72:3666–3670. 5. Wallach D, Kovalenko A. 12th international TNF conference: the good, the bad and the scientists. Cytokine Growth Factor Reveiw 2009;20:259–269. 6. Beutler B, Cerami A. Cachectin and tumour necrosis factor as two sides of the same biological coin. Nature 1986 17;320:584–588. 7. Wajant H, Pfizenmaier K, Scheurich P. Tumor necrosis factor signaling. Cell Death Differentiation 2003;10:45–65. 8. Hunt N, McHale S. The psychological impact of alopecia. British Medical Journal 2005 22;331:951–953. 9. Ghaderian SMH, Akbarzadeh Najar R, Tabatabaei Panah AS. Tumor necrosis factor-α: investigation of gene polymorphism and regulation of TACE-TNF-α system in patients with acute myocardial infarction. Molecular Biology Reports 2011;38:4971–4977. 10. Islam N, Leung PSC, Huntley AC, Gershwin ME. The autoimmune basis of alopecia areata: a comprehensive review. Autoimmunity Review 2015;14:81–89. 11. Maryam A, Hasan S, Zahra H, Pardis K, Sara SR, Hosein AM. Correlation between the severity of alopecia areata and its risk factors. Iran Journal of Dermatology 2011;14:6–11. 12. Kemp EH, McDonagh AJG, Wengraf DA, Messenger AG, Gawkrodger DJ, Cork MJ, Tazi-Ahnini R. The non-synonymous C1858T substitution in the PTPN22 gene is associated with susceptibility to the severe forms of alopecia areata. Human Immunology 2006;67:535–539. 13. Galbraith GM, Pandey JP. Tumor necrosis factor alpha (TNF-alpha) gene polymorphism in alopecia areata. Human Genetics 1995;96:433–436. 14. Elsaid A, Helaly MA, Hatata E-SZ, Fouda O, Settin A. TNF-α-308 and INF-γ+874 Gene Polymorphisms in Relation to Susceptibility and Severity of Type 2 Diabetes Mellitus Elsaid A, Helaly MA, among Egyptian Cases. Electronic Journal of General Medicine 2012 10;9:173–177.
Daneshvar Medicine
Volume 26, Issue 3 - Serial Number 136
September and October 2018
Pages 33-42

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