The effect of high-intensity interval training on physical function and energy intake in male rats consuming a high-fat diet

Document Type : Original Article

Authors

1 Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran

2 Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background and Objective: High-fat diet (HFD) leads to adverse changes in skeletal muscles. Regular exercise reduces skeletal muscle disorders by maintaining physical function. This study aimed to assess the effect of high-intensity interval training (HIIT) on muscle strength, endurance capacity, food consumed, energy intake, and Lee index in male rats consuming a HFD and normal diet (ND).
Materials and Methods: 44 rats were divided into four equal groups, including ND control, HFD control, HIIT+ ND, and HIIT+ HFD. The HIIT was performed for 12 weeks at 85-90% of the maximum speed. Data were analyzed by repeated measures analysis of variance and one-way analysis of variance at a significance level of P<0.05.
Results: Food consumed and energy intake were lower and higher, respectively in the HFD control group compared with the ND control. Also, the body weight, Lee index, food consumed, and energy intake in the HIIT+HFD group were significantly lower than in the HFD control group and Lee index and food consumed in the HIIT+HFD group were significantly lower than in the HIIT+ND group. Furthermore, the endurance capacity in the HIIT+HFD group was significantly higher than in the HFD control group and in the HIIT+ND group was significantly higher than the ND and HFD controls. Absolute and relative muscle strength also increased and decreased in all groups, respectively.
Conclusion: It seems that performing HIIT despite consuming a HFD leads to improved physical function and reduced body weight gain.

Keywords


  1. Ferretti R, Moura EG, Dos Santos VC, Caldeira EJ, Conte M, Matsumura C Y, et al. High-fat diet suppresses the positive effect of creatine supplementation on skeletal muscle function by reducing protein expression of IGF-PI3K-AKT-mTOR pathway. Plos One 2018; 13(10): e0199728.
  2. Lee SR, KhamouiAV, Jo E, Park BS, Zourdos M C, Panton L B, et al. Effects of chronic high-fat feeding on skeletal muscle mass and function in middle-aged mice. Aging Clinical and Experimental Research 2015; 27(4): 403-11.
  3. Andrich DE, Ou Y, Melbouci L, Leduc-Gaudet JP, Auclair N, Mercier J, et al. Altered lipid metabolism impairs skeletal muscle force in young rats submitted to a short-term high-fat diet. Frontiers in Physiology 2018; 9: 1327.
  4. Apablaza P, Bórquez JC, Mendoza R, Silva M, Tapia G, Espinosa A, et al. Exercise induces an augmented skeletal muscle mitochondrial unfolded protein response in a mouse model of obesity produced by a high-Fat diet. International Journal of Molecular Sciences 2023; 24(6): 5654.
  5. Rivas DA, McDonald DJ, Rice NP, Haran PH, Dolnikowski GG, and Fielding, RA. Diminished anabolic signaling response to insulin induced by intramuscular lipid accumulation is associated with inflammation in aging but not obesity. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 2016; 310: 561–69.
  6. Yoshida Y, Tsutaki A, Tamura Y, Kouzaki K, Sashihara K, Nakashima S, et al. Dietary apple polyphenols increase skeletal muscle capillaries in Wistar rats. Physiological Reports 2018; 6(18).
  7. Vargas-Mendoza N, Ángeles-Valencia M, Madrigal-Santillán, EO, Morales-Martínez M, Tirado-Lule JM, Solano-Urrusquieta A, et al. (2020). Effect of silymarin supplementation on physical performance, muscle and myocardium histological changes, bodyweight, and food consumption in rats subjected to regular exercise training. International Journal of Molecular Sciences 2020; 21(20), 7724.
  8. Gómez-Barroso M, Vargas-Vargas MA, Peña-Montes DJ, Cortés-Rojo, C, Saavedra-Molina A, Sánchez-Duarte E, et al. Effect of three different exercise intensities in combination with diazoxide on contraction capacity and oxidative stress of skeletal muscle in obese rats. Biology 2022; 11(9):1367.
  9. Zheng J, Liu W, Zhu X, Ran L, Lang H, Yi L, et al. Pterostilbene enhances endurance capacity via promoting skeletal muscle adaptations to exercise training in rats. Molecules 2020; 25(1): 186.
  10. Seldeen KL, Lasky G, Leiker MM, Pang M, Personius K E, et al. High intensity interval training improves physical performance and frailty in aged mice. The Journal of Gerontology: Biological Sciences 2018; 73(4): 429-437.
  11. Atakan MM, Güzel Y, Bulut S, Koşar N, McConell Gk, Turnagöl, HH. Six HIIT sessions over 5 days increases VO2max, endurance capacity and sub-maximal exercise fat oxidation as much as 6 high-intensity interval training sessions over 2 weeks. The Journal of Sport and Health Science 2020; 10(4): 478-487.
  12. Tsirigkakis S, Mastorakos G, Koutedakis Y, Mougios V, Nevill AM, Pafili Z, et al. Effects of two workload-matched high-intensity interval training protocols on regional body composition and fat oxidation in obese men. Nutrients 2021; 13(4): 1096.
  13. Hariri N, Thibault L. High-fat diet-induced obesity in animal models. Nutrition Research Review 2010; 23(2): 270-299.
  14. Kim JC, Kang, YS, Noh E B, Seo, BW, Seo DY, Park GD, et al. Concurrent treatment with ursolic acid and low-intensity treadmill exercise improves muscle atrophy and related outcomes in rats. The Korean Journal of Physiology & Pharmacology 2018; 22(4): 427-36.
  15. Lambert K, Hokayem M, Thomas C, Fabre O, Cassan C, Bourret A, et al. Combination of nutritional polyphenols supplementation with exercise training counteracts insulin resistance and improves endurance in high-fat diet-induced obese rats. Scientific Reports 2018; 8(1): 2885.
  16. Gopalan V, Yaligar J, Michael N, Kaur K, Anantharaj R, Verma SK et al. (2021). A 12-week aerobic exercise intervention results in improved metabolic function and lower adipose tissue and ectopic fat in high-fat diet fed rats. Bioscience Reports 2021; 41(1).
  17. Pirman T, Lenardič A, Nemec Svete A, Horvat S. Supplementation with> Your< Iron Syrup Corrects Iron Status in a Mouse Model of Diet-Induced Iron Deficiency. Biology 2021; 10(5): 357.
  18. Groussard C, Maillard F, Vazeille E, Barnich N, Sirvent P, Otero YF, et al. Tissue-specific oxidative stress modulation by exercise: A comparison between MICT and HIIT in an obese rat model. Oxidative Medicine and Cellular Longevity 2019.
  19. Qin F, Dong Y, Wang S, Xu M, Wang Z, Qu C, et al. Maximum oxygen consumption and quantification of exercise intensity in untrained male Wistar rats. Scientific Reports 2020; 10(1): 1-8.
  20. Batista ML, Rosa JC, Lopes RD, Lira FS, Martins E, Yamashita AS, et al. Exercise training changes IL-10/TNF-α ratio in the skeletal muscle of post-MI rats. Cytokine 2010; 49(1): 102-108.
  21. Chavanelle V, Boisseau N, Otero YF, Combaret L, Dardevet D, Montaurier C, et al. Effects of high-intensity interval training and moderate-intensity continuous training on glycaemic control and skeletal muscle mitochondrial function in db/db mice. Scientific Reports 2017; 7(1): 1-10.
  22. Delfan, M., Asl, S. G. Comparison of eight weeks of high intensity interval training vs. continuous training on the genes expression of IL-6 and CRP in adipose tissue of diabetic rats induced by high-fat foods and fructose. Research in Medicine: Journal of Research in Medical Sciences 2020; 44(4): 573-79.
  23. Khalafi M, Mohebbi H, Symonds ME, Karimi P, Akbari A, Tabari E, et al. (2020). The impact of moderate-intensity continuous or high-intensity interval training on adipogenesis and browning of subcutaneous adipose tissue in obese male rats. Nutrients 2020; 12(4): 925.
  24. Azagra-Boronat I, Tres A, Massot-Cladera M, Franch À, Castell M, Guardiola F, et al. Lactobacillus fermentum CECT5716 supplementation in rats during pregnancy and lactation impacts maternal and offspring lipid profile, immune system and microbiota. Cells 2020; 9(3): 575.
  25. Deacon RMJ. Measuring the strength of mice. The Journal of Visualized Experiments 2013;76: 2610.
  26. Ahmadabadi F, Saghebjoo M, Huang CJ, Saffari I, Zardast M. The effects of high-intensity interval training and saffron aqueous extract supplementation on alterations of body weight and apoptotic indices in skeletal muscle of 4T1 breast cancer-bearing mice with cachexia. Applied Physiology, Nutrition, and Metabolism 2020; 45(5): 555-63.‏
  27. Lu Y, Li H, Shen SW, Shen ZH, Xu M, Yang CJ, etn al. Swimming exercise increases serum irisin level and reduces body fat mass in high-fat-diet fed Wistar rats. Lipids in Health and Disease 2016;15(1): 1-8.‏
  28. Yaghoobpour Yekani O, Azarbayjani MA, Peeri M, Farzanegi P. (2018). The effect of aerobic training on anthropometric indices of obesity in male rats fed with high fat diet. Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch 2018, 28(1), 31-36.‏
  29. Seo DY, Kwak HB, Lee SR, Cho YS, Song IS, Kim N, et al. Effects of aged garlic extract and endurance exercise on skeletal muscle FNDC-5 and circulating irisin in high-fat-diet rat models. Nutrition Research and Practice 2014; 8(2):177-82.‏
  30. Aliakbari M, Saghebjoo M, Sarir H, Hedayati M. Hydroalcoholic extract of dill and aerobic training prevents high‐fat diet‐induced metabolic risk factors by improving miR‐33 and miR‐223 expression in rat liver. Journal of Food Biochemistry 2022; 46(8): 14195.‏
  31. Castoldi RC, Magalhães AJB, Ozaki G AT, Koike TE, Garcia TA, Camargo RCT, et al. Alterations in Morphology and Aerobic Resistance of Rats Subjected to Different Physical Training Protocols. International Journal of Morphology 2018; 36(4):1472-1479.
  32. Arellanes‐Licea, EDC, Báez‐Ruiz A, Carranza ME, Arámburo C, Luna M, Díaz‐Muñoz M. Daily patterns and adaptation of the ghrelin, growth hormone and insulin‐like growth factor‐1 system under daytime food synchronisation in rats. Journal of Neuroendocrinology 2014; 26(5): 282-95.‏
  33. Seldeen KL, Redae YZ, Thiyagarajan R, Berman RN, Leiker MM, Troen BR. High intensity interval training improves physical performance in aged female mice: A comparison of mouse frailty assessment tools. Mechanisms of Ageing and Development 2019; 180: 49-62.
  34. Poggiogalle E, Rossignon F, Carayon A, Capel F, Rigaudière JP, de Saint Vincent S, et al Deleterious Effect of High-Fat Diet on Skeletal Muscle Performance Is Prevented by High-Protein Intake in Adult Rats but Not in Old Rats. Frontiers in Physiology (2022);12: 2369.‏
  35. Bang HS, Seo DY, Chung YM, Oh KM, Park JJ, Arturo F, et al. Ursolic acid-induced elevation of serum irisin augments muscle strength during resistance training in men. The Korean Journal of Physiology & Pharmacology 2014; 18(5): 441-46.
  36. Hasan NAKAK, Kamal HM, Hussein, ZA. Relation between body mass index percentile and muscle strength and endurance. Egyptian Journal of Medical Human Genetics 2016; 17(4): 367-72.‏
  37. Zavvari F, Karimzadeh F. A review on the behavioral tests for learning and memory assessments in rat. The Neuroscience Journal of Shefaye Khatam 2017; 5(4): 110-24.‏
  38. Zavvari F, Karimzadeh F. (2015). A methodological review of development and assessment of behavioral models of depression in rats. The Neuroscience Journal of Shefaye Khatam 2015; 3(4): 151-160.‏
  39. Grippo AJ, Sullivan NR, Damjanoska KJ, Crane JW, Carrasco GA, Shi J, et al. Chronic mild stress induces behavioral and physiological changes, and may alter serotonin 1A receptor function, in male and cycling female rats. Psychopharmacology 2005; 179: 769-780.‏
  40. Haramizu S, Ota N, Otsuka A, Hashizume K, Sugita S, Hase T, et al. Dietary milk fat globule membrane improves endurance capacity in mice. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2014; 307(8): 1009-1017.‏
  41. Kawanishi N, Takagi K, Lee H C, Nakano D, Okuno T, Yokomizo T, et al. Endurance exercise training and high-fat diet differentially affect composition of diacylglycerol molecular species in rat skeletal muscle. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2018; 314(6): 892-901.
  42. Charlot A, Morel L, Bringolf A, Georg I, Charle AL, Goupilleau F, et al. Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice. Nutrients 2022; 14(13): 2721.
  43. Murase T, Haramizu S, Shimotoyodome A, Nagasawa A, Tokimitsu I. Green tea extract improves endurance capacity and increases muscle lipid oxidation in mice. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2005; 288(3): 708-15.‏
  44. Fleming J, Sharman MJ, Avery NG, Love DM, Gómez AL, Scheett TP, et al. Endurance capacity and high-intensity exercise performance responses to a high-fat diet. International Journal of Sport Nutrition and Exercise Metabolism 2003; 13(4): 466-478.