The efficacy of isolation and culture of human amniotic mesenchymal stem cells in cell culture medium

Document Type : Original Article

Authors

1 Department of Biology, Hamedan Branch, Islamic Azad University, Hamedan, Iran

2 Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background and Objective: He efficacy of methods for amniotic membrane-derived mesenchymal stem cells isolation, culture, and biological characterization faces serious challenges. The present study aimed to investigate the efficacy of methods for amniotic membrane-derived mesenchymal stem cells isolation, culture, and differentiation in cell culture.
Materials and Methods: In this experimental laboratory study, ten placenta specimens were obtained from pregnant mothers during cesarean section and amniotic membranes were separated. The protocol for isolation and culture of mesenchymal cells was optimized by enzymatic method. The morphological characteristics of mesenchymal cells were examined by invert microscopy and biological characteristics were measured by flow cytometry and differentiation capacity was evaluated by mesenchymal cells capacity to differentiate to osteocyte and adipocyte. Data were analyzed using descriptive statistics.
Results: The expression level of CD44, CD73, CD90, and CD29 was significant in cells isolated from the human amniotic cell membrane. CD14, CD34, and CD45 were not expressed or slightly expressed. The cells had high viability and successfully differentiated into osteocyte and adipocyte.
Conclusion: The protocol used in this study to isolate and culture human amniotic membrane-derived mesenchymal stem cells was highly efficient to prepare mesenchymal stem cells for cell therapy and tissue engineering.

Keywords

References

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Daneshvar Medicine
Volume 28, Issue 6 - Serial Number 151
January and February 2021
Pages 1-11

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