The effect of resistance training on testicular function and spermatogenesis process and sperm parameters of adult male Wistar rats

Document Type : Original Article

Authors

1 Department of Exercise Physiology, Faculty of Humanities, Shahed University, Tehran, Iran

2 Neurophysiology Research Center, Shahed University, Tehran, Iran

Abstract

Background and Objective: It has been shown that spermatogenesis disorder could induce infertility in male individuals. This study investigated the effect of resistance training on testicular function, spermatogenesis and sperm parameters in adult male Wistar rats.
Materials and Methods: Twenty adult male rats were selected and randomly divided into two groups; 1- control (n=10) and resistance training (n=10). The exercise was performed three days a week for a period of 5 weeks. We chose the method of 50% of body mass with 30 g overload and a resting time of 90 s. Testosterone of the serum, interstitial testicular fat density, spermatogenesis indexes and other sperm parameters were measured and then analyzed by statistical t-test.
Results: In resistance training groups, there was a significant increase in the number of Leydig cells and interstitial fat density of the testis in comparison with the control group (p < 0.05), but this difference was not shown for the level of serum testosterone. Also, histological analysis showed that resistance training could significantly reduce spermatogenesis indices i.e. tubular differentiation index (TDI), spermiogenesis index (SI) and repopulation index (RI) in comparison with control group (p < 0.05). Nevertheless, this training could not yield the marked changes on number and the diameter of spermatozoon tube and its epitheliums. Finally, our results showed that motility and viability of sperm decrease prominently but, the amount of DNA fracture increased.
Conclusion: Resistance training could disrupt the process of spermatogenesis and sperm parameters that might yield infertility in male individuals.

Keywords


  1. Vander Borght M, Wyns C. Fertility and infertility: Definition and epidemiology. Clinical Biochemistry 2018;62:2-10.
  2. De Kretser D, Baker H. Infertility in men: recent advances and continuing controversies. The Journal of Clinical Endocrinology & Metabolism 1999;84(10):3443-50.
  3. Jungwirth A, Giwercman A, Tournaye H, Diemer T, Kopa Z, Dohle G, et al. European Association of Urology guidelines on Male Infertility: the 2012 update. European Urology 2012;62(2):324-32.
  4. Holstein A-F, Schulze W, Davidoff M. Understanding spermatogenesis is a prerequisite for treatment. Reproductive Biology and Endocrinology 2003;1(1):107.
  5. Ilacqua A, Izzo G, Emerenziani GP, Baldari C, Aversa A. Lifestyle and fertility: the influence of stress and quality of life on male fertility. Reproductive Biology and Endocrinology 2018;16(1):115.
  6. Adamu B, Sani M, Abdu A. Physical exercise and health: a review. Nigerian journal of medicine: journal of the National Association of Resident Doctors of Nigeria 2006;15(3):190-6.
  7. Samadian Z, Tofighi A, Razi M, Tolouei Azar J, Ghaderi Pakdel F. Moderateā€intensity exercise training ameliorates the diabetesā€suppressed spermatogenesis and improves sperm parameters: Insole and simultaneous with insulin. Andrologia 2019;51(11):e13457.
  8. Punhagui APF, Teixeira GR, de Freitas MC, Seraphim PM, Fernandes GSA. Intermittent resistance exercise and obesity, considered separately or combined, impair spermatic parameters in adult male Wistar rats. International Journal of Experimental Pathology 2018;99(2):95-102.
  9. Chigurupati S, Son TG, Hyun D-H, Lathia JD, Mughal MR, Savell J, et al. Lifelong running reduces oxidative stress and degenerative changes in the testes of mice. The Journal of Endocrinology 2008;199(2):333.
  10. Dutra Gonçalves G, Antunes Vieira N, Rodrigues Vieira H, Dias Valério A, Elóisa Munhoz de Lion Siervo G, Fernanda Felipe Pinheiro P, et al. R ole of resistance physical exercise in preventing testicular damage caused by chronic ethanol consumption in UChB rats. Microscopy Research and Technique 2017;80(4):378-86.
  11. Nikbin S, Derakhshideh A, Karimi Jafari S, Mirzahamedani A, Moslehi A, Ourzamani S, et al. Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats. Andrologia 2020;52(2):e13468.
  12. Dutra Gonçalves G, Antunes Vieira N, Rodrigues Vieira H, Dias Valério A, Elóisa Munhoz de Lion Siervo G, Fernanda Felipe Pinheiro P, et al. Role of resistance physical exercise in preventing testicular damage caused by chronic ethanol consumption in UChB rats. Microscopy Research and Technique 2017;80(4):378-86.
  13. Gebreegziabher Y, Marcos E, McKinon W, Rogers G. Sperm characteristics of endurance trained cyclists. International Journal of Sports Medicine 2004;25(04):247-51.
  14. Saremi, Abbas, Membini, Amin. Effect of a swimming training course on the quality of semen fluid spermatogenesis and testicular oxidative stress status in obese mice. Applied Biological Sciences in Sports 2016;3(6):65-73.
  15. Çakir-Atabek H, Demir S, PinarbaSili RD, Gündüz N. Effects of different resistance training intensity on indices of oxidative stress. The Journal of Strength & Conditioning Research 2010;24(9):2491-7.
  16. Talebi-Garakani E, Safarzade A. Resistance training decreases serum inflammatory markers in diabetic rats. Endocrine 2013;43(3):564-70.
  17.  alantari Hesari A, Shahrooz R, Ahmadi A, Malekinejad H, Saboory E. Crocin prevention of anemia-induced changes in structural and functional parameters of mice testes. Journal of Applied Biomedicine. 2015 2015/07/01/;13(3):213-23.
  18. Zirkin BR, Papadopoulos V. Leydig cells: formation, function, and regulation. Biology of reproduction 2018;99(1):101-11.
  19. Hall P, Irby D, Kretser Dd. Conversion of cholesterol to androgens by rat testes: comparison of interstitial cells and seminiferous tubules. Endocrinology 1969;84(3):488-96.
  20. Almahbobi G, Williams L, Han X, Hall P. Binding of lipid droplets and mitochondria to intermediate filaments in rat Leydig cells. Reproduction 1993;98(1):209-17.
  21. Saremi A, Ashtiani SC, shavandi n, Membin A. The effect of different training intensities on spermatogenesis and reproductive hormones in obese mice. Journal of Sports biosciences 2013:81-94.
  22. Mehmandost R, Safarzadeh AR, Rezaei FMM. The effect of increasing resistance training on serum levels of some sex hormones and epididymal sperm count in rats fed sucrose solution. Exercise Physiology 2017:131-46.
  23. Mombeyni A, Bahmanzade M, Sarami A, Changizi-Ashtiyani S, Parastesh M. The Effect of Increasing Resistance Training on Testicular Oxidative Stress and Quality of Spermatogenesis in Male Rats. Journal of Arak University of Medical Sciences 2018;21(4):86-97.
  24. Turner TT, Lysiak JJ. Oxidative stress: a common factor in testicular dysfunction. Journal of Andrology 2008;29(5):488-98.
  25. Breucker H, Schäfer E, Holstein A-F. Morphogenesis and fate of the residual body in human spermiogenesis. Cell and Tissue Research 1985;240(2):303-9.
  26. Sasaki T, Marcon E, McQuire T, Arai Y, Moens PB, Okada H. Bat3 deficiency accelerates the degradation of Hsp70-2/HspA2 during spermatogenesis. The Journal of Cell Biology 2008;182(3):449-58.
  27. Sharp FR, Massa SM, Swanson RA. Heat-shock protein protection. Trends in Neurosciences 1999;22(3):97-9.
  28. Koeva Y DS, Georgieva K, Atanassova P. Heat shock protein- 70 expression in testis following endurance training of rats. Gruev B, Nikolova M, Donev A (eds) Proceedings of the Balkan scientific conference of biology, Plovdiv, Bulgaria 2005: 288–94.
  29. Samadian Z, Tofighi A, Razi M, Tolouei Azar J, Ghaderi Pakdel F. Moderate-intensity exercise training ameliorates the diabetes-suppressed spermatogenesis and improves sperm parameters: Insole and simultaneous with insulin. Andrologia 2019;51(11):e13457.
  30. Maleki BH, Tartibian B. High-intensity exercise training for improving reproductive function in infertile patients: a randomized controlled trial. Journal of Obstetrics and Gynaecology Canada 2017;39(7):545-58.
  31. Saremi, Ashtiani C, kalantari, Abolfazl. Combining vitamin E and intense exercise on testicular oxidative stress and spermatogenesis in male Mice. Sports Physiology (Sports Science Research) 2013;6(23):43-54.
  32. Membini, Amin, Bahmanzadeh, Saremi, Ashtiani C, parastesh. The effect of increasing resistance training on testicular oxidative stress status and spermatogenesis quality in male mice. Journal of Arak University of Medical Sciences 2018;21(4):86-97.
  33. Saremi, Abbas, Ashtiani C, Saeed, shavandi, nader, et al. Effect of different training intensities on spermatogenesis and reproductive hormones in obese mice. Journal of Sports Biological Sciences 2013;5(2):81-94.