Evaluation of biofilm production capacity in salmonella isolated from chicken meat in Tehran municipally daily fruit and vegetable markets

Authors

Abstract

Background and Objective: One of the major issues in food hygiene and safety is the emergence of antibiotic-resistant bacteria. Pathogens as well as biofilm formation can play a role in increasing bacterial resistance. In this study, biofilm formation in Salmonella isolates from chicken, drug resistance and drug resistance pattern were investigated.
Materials and Methods: In this study, 75 samples of chicken infected with Salmonella bacteria were studied. The rate of biofilm formation was assessed by microtiter plate method. Drug resistance and evaluation of drug resistance pattern were also evaluated.
Results: The results showed that in 92% (69/75) of the samples Salmonella biofilm were formed, among which the moderate biofilm with 65.2% had the highest biofilm production. Concurrent resistance to 2 classes of antibiotics (2DR) with 31.8% (22/69) was the most common type of multidrug resistance (MDR) among the biofilm-forming isolates. Resistance to imipenem, ceftriaxone and cefotaxime was observed in only 1 biofilm formation sample.
Conclusion: In this study, high biofilm formation was observed in Salmonella contaminated chicken meat samples. The high rate of antibiotic resistance in biofilm constituents is due to the increasing attention to hygienic aspects of non-transmission of food contamination to humans.

Keywords


1. Da Silva N, Taniwaki MH, Junqueira VC, Silveira N, Okazaki MM, Gomes RA. Microbiological examination methods of food and water: a laboratory manual. The Chemical Rubber Company Press 2018. 2. Temelli S, Eyigor A, Carli KT. Salmonella serogroup detection in poultry meat samples by examining multiple colonies from selective plates of two standard culture methods. Foodborne Pathogens And Disease 2010;17(10):1229-34. 3. Chiu Lh, Chiu Ch, Horn Ym, Chiou Cs, Lee Cy, Yeh Cm, Yu Cy, Wu Cp, Chang CC, Chu C. Characterization of 13 multi-drug resistant Salmonella serovars from different broiler chickens associated with those of human isolates. Microbiology 2010;10(1):86. 4. Jones K, Bradshaw SB. Biofilm formation by the Enterobacteriaceae: a comparison between Salmonella enteritidis, Escherichia coli and a nitrogen‐fixing strain of Klebsiella pneumoniae. Journal of Applied Bacteriology 1996;80(4):458-64. 5. Dhir VK, Dodd CE. Susceptibility of suspended and surface-attached Salmonella enteritidis to biocides and elevated temperatures. Applied and Environmental Microbiology 1995;1;61(5):1731-8. 6. Austin JW, Sanders G, Kay WW, Collinson SK. Thin aggregative fimbriae enhance Salmonella enteritidis biofilm formation. FEMS Microbiology Letters 1998;162(2):295-301. 7. Palmer RJ, White DC. Developmental biology of biofilms: implications for treatment and control. Trends in Microbiology 1997;1;5(11):435-40. 8. Atshan SS, Nor Shamsudin M, Sekawi Z, Lung LT, Hamat RA, Karunanidhi A, et al. Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus. BioMed Research International 2012. 9. Lewis K. Multidrug tolerance of biofilms and persister cells. In Bacterial Biofilms. Springer, Berlin, Heidelberg 2008. 10. Kim SH, Wei CI. Biofilm formation by multidrug-resistant Salmonella enterica serotype Typhimurium phage type DT104 and other pathogens. Journal of Food Protection 2007;70(1):22-9. 11. Arciola CR, Campoccia D, Speziale P, Montanaro L, Costerton JW. Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials. Biomaterials 2012; 33(26):5967-82. 12. Srey S, Jahid IK, Ha S-D. Biofilm formation in food industries: A food safety concern. Food Control 2013; 31(2):572-85. 13. Palmer J, Flint S, Brooks J. Bacterial cell attachment, the beginning of a biofilm. Journal of Industrial Microbiology & Biotechnology 2007; 34(9):577-88. 14. Gupta P, Sarkar S, Das B, Bhattacharjee S, Tribedi P. Biofilm, pathogenesis and prevention a journey to break the wall: a review. Archives of Microbiology 2016; 1;198(1):1-5. 15. Mikoleit M. Laboratory protocol: biochemical identification of Salmonella and Shigella using an abbreviated panel of tests. Geneva, Switzerland: WHO Global Foodborne Infections Network 2010. 16. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. Clinical and Laboratory Standards Institute 28th ed 2018. 17. Zmantar T, Kouidhi B, Miladi H, Mahdouani K, Bakhrouf A. A microtiter plate assay for Staphylococcus aureus biofilm quantification at various pH levels and hydrogen peroxide supplementation. The New Microbiology 2010; 33(2):137-45. 18. Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Journal of Pathology, Microbiology and Immunology 2007; 115(8):891-9. 19. Manyi-Loh C, Mamphweli S, Meyer E, Okoh A. Antibiotic use in agriculture and its consequential resistance in environmental sources: Potential Public Health Implications. Molecules 2018; 23(4):795. 20. Besharati S, Owlia P, Sadeghi A, Ahmadi F, Fani F, Puladfar G, Alebouyeh M. Frequency, antibiotic resistance, and serogroups of Salmonella among chicken meat specimens in Tehran, Iran. Daneshvar Medicine 2019; 10;27(143):1-0. 21. Ghasemmahdi H, Tajik H, Moradi M, Mardani K, Modaresi R, Badali A, Dilmaghani M. Antibiotic resistance pattern and biofilm formation ability of clinically isolates of Salmonella enterica serotype typhimurium. International Journal of Enteric Pathogens 2015; 3(2):4-27372. 22. Shi X, Zhu X. Biofilm formation and food safety in food industries. Trends in Food Science & Technology 2009; 1;20(9):407-13. 23. Ioannidis A, Papavasileiou K, Papavasileiou E, Bersimis S, Chatzipanagiotou S. Distribution of six effector protein virulence genes among Salmonellaenterica enterica serovars isolated from children and their correlation with biofilm formation and antimicrobial resistance. Molecular Diagnosis & Therapy 2013; 1;17(5):311-7. 24. Kim MS, Lim TH, Jang JH, Lee DH, Kim BY, Kwon JH, et al. Prevalence and antimicrobial resistance of Salmonella species isolated from chicken meats produced by different integrated broiler operations in Korea. Poultry Science 2012; 91(9):2370-5. 25. Mahdavi MA, Kasra Kermanshahi R, Jalali MO. Biofilm formation of Salmonella enteritidis on surfaces in the food industry. Iranian Journal of Nutrition Sciences & Food Technology 2008; 3(2):81-4. 26. Sirdani A, Soltan Dallal MM. Investigating the Ability of Producing Biofilm by Isolated Salmonella from Food. Alborz University Medical Journal 2018;10;7(4):309-14. 27. Abdallah FB, Lagha R, Khaled SA, Kallel H, Gharbi J. Detection of cell surface hydrophobicity, biofilm and fimbirae genes in Salmonella isolated from tunisian clinical and poultry meat. Iranian Journal of Public Health 2014; 43(4):423.