Evaluation of percentage of regulatory T cells in peripheral blood of sulfur mustard-exposed in comparison with healthy individuals

Authors

Abstract

Background and Objective: Regulatory T cells (Treg) are the subgroups of lymphocytes that control inflammation response and regulates homeostasis by mechanisms of cellular contact and secretion of soluble agents. Here, we investigated percentage of Treg in peripheral blood of sulfur mustard-exposed patients with CD4, CD25, CD127, FOXP3 markers in comparison with healthy individuals and the correlation between these cells and long-term pulmonary complications.
 
Materials and Methods: In this study, 12 sulfur mustard-exposed patients and 12 healthy volunteers were invited. Clinical inspections of both groups were done by pulmonary specialists as well as spirometric evaluation that was conducted by pulmonary function test operators. After isolation of peripheral blood mononuclear cells, the percentage of Treg cells was determined by flow cytometry.
 
Results: Percentage of Treg cells was not significantly different in those exposed to sulfur mustard. Also, there was no significant correlation between spirometric parameters and percentage of these cells.
 
Conclusion: According to the current study, there is no difference in the percentage of Treg cells between exposed patients with mild pulmonary complications and healthy volunteers and more studies are needed to understand the role of these cells in the pulmonary complications of these patients.
 

Keywords


1. Mansour Razavi S, Salamati P, Saghafinia M, Abdollahi M. A review on delayed toxic effects of sulfur mustard in Iranian veterans. DARU Journal of Pharmaceutical Sciences 2012;20(1):51. 2. Emami MH, Talaei M, Panahi Y, Saburi A, Ghanei M. Efficacy of omeprazole on cough, pulmonary function and quality of life of patients with sulfur mustard lung injury: A placebo-control, cross-over clinical trial study. Journal of Research in Medical Sciences: The official Journal of Isfahan University of Medical Sciences 2014;19(11):1027. 3. Imani S, Salimian J, Fu J, Ghanei M, Panahi Y. Th17/Treg-related cytokine imbalance in sulfur mustard exposed and stable chronic obstructive pulmonary (COPD) patients: correlation with disease activity. Immunopharmacology and Immunotoxicology 2016;38(4):270-80. 4. Emad A, Emad Y. Levels of cytokine in bronchoalveolar lavage (BAL) fluid in patients with pulmonary fibrosis due to sulfur mustard gas inhalation. Journal of Interferon & Cytokine Research 2007;27(1):38-43. 5. Ghazanfari T, Kariminia A, Yaraee R, Faghihzadeh S, Ardestani SK, Ebtekar M, et al. Long term impact of sulfur mustard exposure on peripheral blood mononuclear subpopulations—Sardasht-Iran Cohort Study (SICS). International Immunopharmacology 2013;17(3):931-5. 6. Shaker Z, Hassan Z, Sohrabpoor H, Mosaffa N. The immunostatus of T helper and T cytotoxic cells in the patients ten years after exposure to sulfur mustard. Immunopharmacology and Immunotoxicology 2003;25(3):423-30. 7. Imani S, Salimian J, Bozorgmehr M, Vahedi E, Ghazvini A, Ghanei M, et al. Assessment of Treg/Th17 axis role in immunopathogenesis of chronic injuries of mustard lung disease. Journal of Receptors and Signal Transduction 2016;36(5):531-41. 8. Roos-Engstrand E, Pourazar J, Behndig AF, Bucht A, Blomberg A. Expansion of CD4+ CD25+ helper T cells without regulatory function in smoking and COPD. Respiratory Research 2011;12(1):74. 9. Santegoets SJ, Dijkgraaf EM, Battaglia A, Beckhove P, Britten CM, Gallimore A, et al. Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry. Cancer Immunology, Immunotherapy 2015;64(10):1271-86. 10. Singer BD, King LS, D'Alessio FR. Regulatory T cells as immunotherapy. Frontiers in Immunology 2014;5:46. 11. Letourneau S, Krieg C, Pantaleo G, Boyman O. IL-2- and CD25-dependent immunoregulatory mechanisms in the homeostasis of T-cell subsets. The Journal of Allergy and Clinical Immunology 2009;123(4):758-62. 12. Seddiki N, Santner-Nanan B, Martinson J, Zaunders J, Sasson S, Landay A, et al. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. Journal of Experimental Medicine 2006;203(7):1693-700. 13. Li Z, Li D, Tsun A, Li B. FOXP3+ regulatory T cells and their functional regulation. Cellular & Molecular Immunology 2015;12(5):558. 14. Sawant DV, Vignali DA. Once a Treg, always a Treg? Immunological Reviews 2014;259(1):173-91. 15. Paats MS, Bergen IM, Hoogsteden HC, van der Eerden MM, Hendriks RW. Systemic CD4+ and CD8+ T cell cytokine profiles correlate with GOLD stage in stable COPD. European Respiratory Journal 2012:erj00796-2011. 16. Barcelo B, Pons J, Ferrer J, Sauleda J, Fuster A, Agusti A. Phenotypic characterisation of T-lymphocytes in COPD: abnormal CD4+ CD25+ regulatory T-lymphocyte response to tobacco smoking. European Respiratory Journal 2008;31(3):555-62. 17. Eusebio M, Kuna P, Kraszula L, Kupczyk M, Pietruczuk M. The relative values of CD8+ CD25+ FOXP3brigh Treg cells correlate with selected lung function parameters in asthma. International Journal of Immunopathology and Pharmacology 2015;28(2):218-26.