The effect of immobilization stress and Aloe vera extract on serum levels of LH, FSH and testosterone in male rats

Authors

Abstract

 Background and Objective: Studies showed that Aloe vera
extract can influence reproductive functions in animals. The purpose of this
study was to determine the effect of immobilization stress and Aloe vera
extract on serum LH, FSH and testosterone levels in male rats.   Materials and Methods: In this study, male Wistar rats were randomly divided into 9 groups including control, Aloe
vera extract receiving, Aloe vera extract and normal saline
receiving, acutely or chronically immobilized, acutely or chronically
immobilized and normal saline receiving and acutely or chronically
immobilized and Aloe vera extract receiving animals of 5 in each group.
Chronic immobilization was exerted 2h/day for 3 weeks and acute immobilization
was exerted 8h/day for one week. Aloe vera extract (625 mg/kg/body
weight) was taken by gavage feeding. Finally, blood samples were collected
using cardiac puncture method. Following serum collection, LH, FSH and
testosterone levels were measured by radioimmunoassay method. Data were compared between groups using
ANOVA.    Results: Serum LH or testosterone level significantly
decreased in chronically or acutely immobilized rats compared to control
animals (p < 0.001) however, LH or testosterone serum level did not
significantly change in Aloe vera extract receiving and acutely or
chronically immobilized Aloe vera extract receiving animals compared
with control rats.   Conclusion: Our findings showed that immobilization stress
reduces serum level of LH and testosterone however, intake of Aloe vera
can withstand against. 

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