Shahed UniversityDaneshvar Medicine2716-972331520240104The effect of Urtica dioica in combination with endurance training on testicular spermatogenic factors in streptozotocin-induced diabetic ratsThe effect of Urtica dioica in combination with endurance training on testicular spermatogenic factors in streptozotocin-induced diabetic rats111430710.22070/daneshmed.2024.17830.1358FAFatemehMalakoutiniaDepartment of Physical Education and Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran0000-0002-4711-828XMasoudRahmatiDepartment of Physical Education and Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran0000-0003-4792-027XRahimMirnasouriDepartment of Physical Education and Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran0000-0002-9557-2081LeilaZareiDepartment of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran0000-0001-8753-3667Journal Article20230608<strong>Background and Objective:</strong> Today, diabetes is a major health problem with an increasing prevalence in men. It has been stated that high glucose leads to increased oxidative stress and apoptosis in testicular cells and causes testicular cell death and infertility. This study was conducted to investigate and determine the effect of nettle hydroalcoholic extract and endurance exercises on testicular tissue and spermatogenesis factors in streptozotocin (STZ)-diabetic male rats.<br /><strong>Materials and Methods:</strong> In this experimental study, 40 albino rats weighing 200-220 grams were randomly divided into 5 groups: control-healthy (HC), nettle-diabetes (DG), exercise-diabetes (DT, nettle-exercise), diabetes (DTG), and control diabetes (DC). Endurance training was performed for 6 weeks according to the protocol. At the same time, rats of DG and DTG groups received nettle hydroalcoholic extract at a dose of 45 mg per kg of body weight. After 6 weeks, the testes were removed and stained with H&E technique.<br /><strong>Results</strong><strong>:</strong> In the DTG and the DG groups, both spermatogenesis index (SI) and repopulation index (RI) were higher compared to the DT group and were close to the results of the healthy control group. Also, the histopathology of the testis in these two groups showed a higher improvement compared to the DT group and similar results were obtained with the results of the HC group.<br /><strong>Conclusion: </strong>The present study showed that diabetes has destructive effects on spermatogenesis and testicular tissue and administration of nettle and endurance exercises together can protect spermatogenic cells in seminiferous tubules from the harmful effects of diabetes by improving SI and RI percentages.<strong>Background and Objective:</strong> Today, diabetes is a major health problem with an increasing prevalence in men. It has been stated that high glucose leads to increased oxidative stress and apoptosis in testicular cells and causes testicular cell death and infertility. This study was conducted to investigate and determine the effect of nettle hydroalcoholic extract and endurance exercises on testicular tissue and spermatogenesis factors in streptozotocin (STZ)-diabetic male rats.<br /><strong>Materials and Methods:</strong> In this experimental study, 40 albino rats weighing 200-220 grams were randomly divided into 5 groups: control-healthy (HC), nettle-diabetes (DG), exercise-diabetes (DT, nettle-exercise), diabetes (DTG), and control diabetes (DC). Endurance training was performed for 6 weeks according to the protocol. At the same time, rats of DG and DTG groups received nettle hydroalcoholic extract at a dose of 45 mg per kg of body weight. After 6 weeks, the testes were removed and stained with H&E technique.<br /><strong>Results</strong><strong>:</strong> In the DTG and the DG groups, both spermatogenesis index (SI) and repopulation index (RI) were higher compared to the DT group and were close to the results of the healthy control group. Also, the histopathology of the testis in these two groups showed a higher improvement compared to the DT group and similar results were obtained with the results of the HC group.<br /><strong>Conclusion: </strong>The present study showed that diabetes has destructive effects on spermatogenesis and testicular tissue and administration of nettle and endurance exercises together can protect spermatogenic cells in seminiferous tubules from the harmful effects of diabetes by improving SI and RI percentages.https://daneshvarmed.shahed.ac.ir/article_4307_38087d90fe0083a5d17fef54ed2b3220.pdfShahed UniversityDaneshvar Medicine2716-972331520240105Knowledge, attitude, and performance of medical students of Shahed University in prevention of Covid-19 in 2021Knowledge, attitude, and performance of medical students of Shahed University in prevention of Covid-19 in 20211222430810.22070/daneshmed.2024.17977.1377FAMaryamAhmadifardDepartment of Health and Social Medicine, School of Medicine, Shahed University, Tehran, Iran0000-0003-0617-6333ZahraJouhariDepartment of Health and Social Medicine, School of Medicine, Shahed University, Tehran, Iran0000-0003-0484-1811Journal Article20230716<strong>Background and Objective:</strong> Covid-19 pandemic that occurred in 2019 was a critical health issue worldwide. Medical students should be evaluated in terms of awareness, attitude and performance towards this pandemic. This study was designed and implemented with the aim of investigating the knowledge, attitude and performance of medical students of Shahed University.
<strong>Materials and Methods:</strong> This cross-sectional study was conducted in 2021. All medical students of Shahed University who met the inclusion criteria were included in the study after sampling. These students completed the questionnaire of knowledge, attitude and performance towards the prevention of covid-19, whose validity and reliability were measured. The information of these questionnaires was entered into SPSS software version 26 and subjected to statistical analysis.
<strong>Results</strong><strong>:</strong> In this study, 170 students were included. The average score of students' knowledge, attitude and performance was 20.70 (out of 25), 11.42 (13) and 53.50 (out of 60), respectively. There was a significant relationship between mean score of knowledge, attitude and performance towards the prevention of Covid-19 in students according to gender, marital status and educational level (P<0.05). Increased awareness was associated with increased attitude and performance.
<strong>Conclusion: </strong>: This study showed that the medical students of Shahed University have good and high knowledge, attitude and performance towards the prevention of Covid-19 disease.<strong>Background and Objective:</strong> Covid-19 pandemic that occurred in 2019 was a critical health issue worldwide. Medical students should be evaluated in terms of awareness, attitude and performance towards this pandemic. This study was designed and implemented with the aim of investigating the knowledge, attitude and performance of medical students of Shahed University.
<strong>Materials and Methods:</strong> This cross-sectional study was conducted in 2021. All medical students of Shahed University who met the inclusion criteria were included in the study after sampling. These students completed the questionnaire of knowledge, attitude and performance towards the prevention of covid-19, whose validity and reliability were measured. The information of these questionnaires was entered into SPSS software version 26 and subjected to statistical analysis.
<strong>Results</strong><strong>:</strong> In this study, 170 students were included. The average score of students' knowledge, attitude and performance was 20.70 (out of 25), 11.42 (13) and 53.50 (out of 60), respectively. There was a significant relationship between mean score of knowledge, attitude and performance towards the prevention of Covid-19 in students according to gender, marital status and educational level (P<0.05). Increased awareness was associated with increased attitude and performance.
<strong>Conclusion: </strong>: This study showed that the medical students of Shahed University have good and high knowledge, attitude and performance towards the prevention of Covid-19 disease.https://daneshvarmed.shahed.ac.ir/article_4308_8cb94e7a9661ea20b1293c589216d396.pdfShahed UniversityDaneshvar Medicine2716-972331520240105Comparison of the effect of 6-week aerobic and high intensity interval training on expression of uPA and uPAR in the tumor tissue of female mice with breast cancerComparison of the effect of 6-week aerobic and high intensity interval training on expression of uPA and uPAR in the tumor tissue of female mice with breast cancer2334430910.22070/daneshmed.2024.18022.1381FAGitaFardadDepartment of Sport Sciences, University of Tehran, Kish International Campus, Kish, Iran0000-0001-7377-0231AbasaliGaeiniDepartment of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, IranRezaNouriDepartment of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, IranParisaPournematiDepartment of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran0000-0002-4528-5105Journal Article20230731<strong>Background and Objective:</strong> In the past few decades, research on the role of physical activity in the prevention of cancer and disease progression has been the subject of attention of many researchers. The aim of this study was to compare the effect of 6 weeks of aerobic training and high intensity interval training on the expression of uPA and uPAR in the tumor tissue of female mice with breast cancer.
<strong>Materials and Methods:</strong> 24 female Balb c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor + aerobic training (T+AE) and tumor + high intensity interval training (T+HIIT). The T+AE group had incremental endurance treadmill running for 6 weeks/5 days/60 minutes. The T+HIIT group had a 6-week/3-day/60 min high intensity interval training protocol. Then, the mice were killed, the tumor tissue was removed and frozen in liquid nitrogen. The expression of uPA and uPAR genes was measured by Real Time-PCR method. ΔCT, ΔΔCT, fold change, one-way ANOVA and Tukey's tests were performed at a significant level P<0.05 with GENEX software .
<strong>Results</strong><strong>:</strong> The results of the present study showed that in the T+AE group compared to the T group, the expression of uPA and uPAR decreased (P=0.00) 2.07 and 2.55 times. In the T+HIIT group compared to the T group, the expression of uPA and uPAR decreased 4.25 and 7.74 times (P=0.00), and in the T+HIIT group compared to the T+AE group, the expression of uPA and uPAR decreased 2.04 and 3.03 times (P=0.00).
<strong>Conclusion: </strong>Aerobic training and high intensity interval training are probably effective in preventing the progression of breast cancer by reducing the expression of uPA and uPAR.<strong>Background and Objective:</strong> In the past few decades, research on the role of physical activity in the prevention of cancer and disease progression has been the subject of attention of many researchers. The aim of this study was to compare the effect of 6 weeks of aerobic training and high intensity interval training on the expression of uPA and uPAR in the tumor tissue of female mice with breast cancer.
<strong>Materials and Methods:</strong> 24 female Balb c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor + aerobic training (T+AE) and tumor + high intensity interval training (T+HIIT). The T+AE group had incremental endurance treadmill running for 6 weeks/5 days/60 minutes. The T+HIIT group had a 6-week/3-day/60 min high intensity interval training protocol. Then, the mice were killed, the tumor tissue was removed and frozen in liquid nitrogen. The expression of uPA and uPAR genes was measured by Real Time-PCR method. ΔCT, ΔΔCT, fold change, one-way ANOVA and Tukey's tests were performed at a significant level P<0.05 with GENEX software .
<strong>Results</strong><strong>:</strong> The results of the present study showed that in the T+AE group compared to the T group, the expression of uPA and uPAR decreased (P=0.00) 2.07 and 2.55 times. In the T+HIIT group compared to the T group, the expression of uPA and uPAR decreased 4.25 and 7.74 times (P=0.00), and in the T+HIIT group compared to the T+AE group, the expression of uPA and uPAR decreased 2.04 and 3.03 times (P=0.00).
<strong>Conclusion: </strong>Aerobic training and high intensity interval training are probably effective in preventing the progression of breast cancer by reducing the expression of uPA and uPAR.https://daneshvarmed.shahed.ac.ir/article_4309_f2b5e92f61b6de923b063588ee6e7c48.pdfShahed UniversityDaneshvar Medicine2716-972331520240105The effect of high-intensity interval training on physical function and energy intake in male rats consuming a high-fat dietThe effect of high-intensity interval training on physical function and energy intake in male rats consuming a high-fat diet3548431010.22070/daneshmed.2024.18065.1383FAAsiyehTaji TabasDepartment of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran0009-0001-9323-5065MarziyehSaghebjooDepartment of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran0000-0001-5862-5490MehdiHedayatiCellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran0000-0001-5816-775xRezaGhahremaniDepartment of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran0000-0002-3691-7641Journal Article20230812<strong>Background and Objective:</strong> High-fat diet (HFD) leads to adverse changes in skeletal muscles. Regular exercise reduces skeletal muscle disorders by maintaining physical function. This study aimed to assess the effect of high-intensity interval training (HIIT) on muscle strength, endurance capacity, food consumed, energy intake, and Lee index in male rats consuming a HFD and normal diet (ND).<br /><strong>Materials and Methods:</strong> 44 rats were divided into four equal groups, including ND control, HFD control, HIIT+ ND, and HIIT+ HFD. The HIIT was performed for 12 weeks at 85-90% of the maximum speed. Data were analyzed by repeated measures analysis of variance and one-way analysis of variance at a significance level of P<0.05.<br /><strong>Results</strong><strong>:</strong> Food consumed and energy intake were lower and higher, respectively in the HFD control group compared with the ND control. Also, the body weight, Lee index, food consumed, and energy intake in the HIIT+HFD group were significantly lower than in the HFD control group and Lee index and food consumed in the HIIT+HFD group were significantly lower than in the HIIT+ND group. Furthermore, the endurance capacity in the HIIT+HFD group was significantly higher than in the HFD control group and in the HIIT+ND group was significantly higher than the ND and HFD controls. Absolute and relative muscle strength also increased and decreased in all groups, respectively.<br /><strong>Conclusion: </strong>It seems that performing HIIT despite consuming a HFD leads to improved physical function and reduced body weight gain.<strong>Background and Objective:</strong> High-fat diet (HFD) leads to adverse changes in skeletal muscles. Regular exercise reduces skeletal muscle disorders by maintaining physical function. This study aimed to assess the effect of high-intensity interval training (HIIT) on muscle strength, endurance capacity, food consumed, energy intake, and Lee index in male rats consuming a HFD and normal diet (ND).<br /><strong>Materials and Methods:</strong> 44 rats were divided into four equal groups, including ND control, HFD control, HIIT+ ND, and HIIT+ HFD. The HIIT was performed for 12 weeks at 85-90% of the maximum speed. Data were analyzed by repeated measures analysis of variance and one-way analysis of variance at a significance level of P<0.05.<br /><strong>Results</strong><strong>:</strong> Food consumed and energy intake were lower and higher, respectively in the HFD control group compared with the ND control. Also, the body weight, Lee index, food consumed, and energy intake in the HIIT+HFD group were significantly lower than in the HFD control group and Lee index and food consumed in the HIIT+HFD group were significantly lower than in the HIIT+ND group. Furthermore, the endurance capacity in the HIIT+HFD group was significantly higher than in the HFD control group and in the HIIT+ND group was significantly higher than the ND and HFD controls. Absolute and relative muscle strength also increased and decreased in all groups, respectively.<br /><strong>Conclusion: </strong>It seems that performing HIIT despite consuming a HFD leads to improved physical function and reduced body weight gain.https://daneshvarmed.shahed.ac.ir/article_4310_c96c08f8bb7960e11a1239352a479053.pdfShahed UniversityDaneshvar Medicine2716-972331520240105Interactive effect of spinning training and capsaicin consumption on body composition and Vo2 max in overweight adolescentsInteractive effect of spinning training and capsaicin consumption on body composition and Vo2 max in overweight adolescents4961431110.22070/daneshmed.2024.18179.1394FADanaSalar Abdellah HamidDepartment of Sports Physiology, Faculty of Physical Education & Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran0009-0008-2951-9777AllahyarArabmomeniDepartment of Sports Sciences, Faculty of Humanities, Khomeinishahr Branch, Islamic Azad University, Isfahan, Iran0000-0003-3658-1006Journal Article20230909<strong>Background and Objective:</strong> Being overweight and obese during adolescence is associated with a wide range of complications and an increased risk of sudden occurrence of physical problems, mental disorders, and some cancers. Therefore, the purpose of this study was to investigate the effect of spinning training and capsaicin consumption on body composition and VO2 max in overweight adolescents.<br /><strong>Materials and Methods:</strong> In this quasi-experimental study, with a pre- and post-test design and a placebo group, 60 overweight teenagers with BMI=26.52±1.38 kg/m2 were selected purposefully and using a systematic random method were divided into 4 groups of 15 people (Exercise, Supplement, Combined, and Placebo). The spinning training protocol was performed for 10 weeks and 3 sessions per week. Furthermore, supplement and combined groups consumed capsules containing 500 mg of capsaicin extract with the usual breakfast for 10 weeks. The measurement of height, weight, BMI, and Vo2 max was done in two stages, pre-test, and post-test. In order to analyze the data, multivariate covariance, and post hoc Bonferroni tests were used using SPSS26 software at a significant level (P<0.05).<br /><strong>Results</strong><strong>:</strong> The results of the study showed that the exercise and combined (Exercise+Supplement) interventions caused a significant reduction in weight, fat percentage, and BMI of overweight teenagers (p=0.001). In addition, a significant increase in Vo2 max was observed in both exercise and combined training groups (p=0.001). Moreover, the changes caused by the combined group on all variables were significantly more than other groups.<br /><strong>Conclusion: </strong>These findings indicate the beneficial effects of the interactive effect of spinning exercise and capsaicin consumption on body composition and VO2 max. Therefore, using spinning exercises and capsaicin herbal supplements is recommended as a suitable method to lose weight in overweight teenagers.<strong>Background and Objective:</strong> Being overweight and obese during adolescence is associated with a wide range of complications and an increased risk of sudden occurrence of physical problems, mental disorders, and some cancers. Therefore, the purpose of this study was to investigate the effect of spinning training and capsaicin consumption on body composition and VO2 max in overweight adolescents.<br /><strong>Materials and Methods:</strong> In this quasi-experimental study, with a pre- and post-test design and a placebo group, 60 overweight teenagers with BMI=26.52±1.38 kg/m2 were selected purposefully and using a systematic random method were divided into 4 groups of 15 people (Exercise, Supplement, Combined, and Placebo). The spinning training protocol was performed for 10 weeks and 3 sessions per week. Furthermore, supplement and combined groups consumed capsules containing 500 mg of capsaicin extract with the usual breakfast for 10 weeks. The measurement of height, weight, BMI, and Vo2 max was done in two stages, pre-test, and post-test. In order to analyze the data, multivariate covariance, and post hoc Bonferroni tests were used using SPSS26 software at a significant level (P<0.05).<br /><strong>Results</strong><strong>:</strong> The results of the study showed that the exercise and combined (Exercise+Supplement) interventions caused a significant reduction in weight, fat percentage, and BMI of overweight teenagers (p=0.001). In addition, a significant increase in Vo2 max was observed in both exercise and combined training groups (p=0.001). Moreover, the changes caused by the combined group on all variables were significantly more than other groups.<br /><strong>Conclusion: </strong>These findings indicate the beneficial effects of the interactive effect of spinning exercise and capsaicin consumption on body composition and VO2 max. Therefore, using spinning exercises and capsaicin herbal supplements is recommended as a suitable method to lose weight in overweight teenagers.https://daneshvarmed.shahed.ac.ir/article_4311_a787f02ed34fd886eb6d49e60d9c9120.pdfShahed UniversityDaneshvar Medicine2716-972331520240105Striatal oxidative stress and inflammation and lower expression of miRNA-149 in ischemic-reperfusion murine model of strokeStriatal oxidative stress and inflammation and lower expression of miRNA-149 in ischemic-reperfusion murine model of stroke6575431210.22070/daneshmed.2024.18201.1398FASamiraVahidiDepartment of Animal Science and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranInstitute for Cognitive and Brain Science, Shahid Beheshti University, Tehran, Iran0009-0000-9269-2945Mohammad RezaBigdeliIranInstitute for Cognitive and Brain Science, Shahid Beheshti University, Tehran, Iran0000-0003-4331-1047HoseinShahsavaraniDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran0000-0002-8123-0857SalmaAhmadlouDepartment of Animal Science and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran0000-0001-9952-299XMehrdadRoghaniNeurophysiology Research Center, Shahed University, Tehran, Iran0000-0002-9209-8484Journal Article20230913<strong>Background and Objective:</strong> Ischemic stroke is one of the main causes of death and disability in the world. Despite advancements, treatments have not achieved the desired results yet. Since microRNAs can be promising candidates for ischemic stroke diagnosis and treatment, this study investigated the impact of ischemic stroke on oxidative stress, inflammation, and miRNA149-5p expression in the striatum region in a rat model of ischemia-reperfusion brain injury.<br /><strong>Materials and Methods:</strong> In this research, 42 Male Wistar rats were divided into sham and ischemic groups. Using the middle cerebral artery occlusion (MCAO) model, cerebral ischemia was induced in the target group. After 24 hours, neurological deficits, cerebral edema, blood-brain barrier permeability, miRNA149-5p expression, and enzymes related to oxidative stress and inflammation were evaluated. Data were statistically analyzed using GraphPad Prism software and unpaired t test.<br /><strong>Results</strong><strong>:</strong> The results of this study indicated that ischemic stroke leads to a decrease in neurological function, increased cerebral edema, and higher blood-brain barrier permeability. Furthermore, it resulted in elevated levels of oxidative and inflammatory factors and reduced levels of antioxidant factors in the striatum region. Additionally, ischemic stroke significantly caused downregulation of miRNA149-5p expression.<br /><strong>Conclusion: </strong>This study demonstrated that miRNA149-5p changes in rats subjected to ischemic stroke. This finding could contribute to a better understanding of therapeutic and diagnostic methods for stroke.<strong>Background and Objective:</strong> Ischemic stroke is one of the main causes of death and disability in the world. Despite advancements, treatments have not achieved the desired results yet. Since microRNAs can be promising candidates for ischemic stroke diagnosis and treatment, this study investigated the impact of ischemic stroke on oxidative stress, inflammation, and miRNA149-5p expression in the striatum region in a rat model of ischemia-reperfusion brain injury.<br /><strong>Materials and Methods:</strong> In this research, 42 Male Wistar rats were divided into sham and ischemic groups. Using the middle cerebral artery occlusion (MCAO) model, cerebral ischemia was induced in the target group. After 24 hours, neurological deficits, cerebral edema, blood-brain barrier permeability, miRNA149-5p expression, and enzymes related to oxidative stress and inflammation were evaluated. Data were statistically analyzed using GraphPad Prism software and unpaired t test.<br /><strong>Results</strong><strong>:</strong> The results of this study indicated that ischemic stroke leads to a decrease in neurological function, increased cerebral edema, and higher blood-brain barrier permeability. Furthermore, it resulted in elevated levels of oxidative and inflammatory factors and reduced levels of antioxidant factors in the striatum region. Additionally, ischemic stroke significantly caused downregulation of miRNA149-5p expression.<br /><strong>Conclusion: </strong>This study demonstrated that miRNA149-5p changes in rats subjected to ischemic stroke. This finding could contribute to a better understanding of therapeutic and diagnostic methods for stroke.https://daneshvarmed.shahed.ac.ir/article_4312_27d8d40b22f812a1ba6c26f8ef7df480.pdfShahed UniversityDaneshvar Medicine2716-972331520240105Skeletal muscle metabolomics analysis after high-intensity interval training in rats fed a high-fat dietSkeletal muscle metabolomics analysis after high-intensity interval training in rats fed a high-fat diet6991431310.22070/daneshmed.2024.18381.1415FAMansourehKarimiDepartment of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran0009-0000-1271-4965MarziyehSaghebjooDepartment of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran0000-0001-5862-5490HadiSarirDepartment of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran0000-0001-6073-9315MehdiHedayatiCellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran0000-0001-5816-775xJournal Article20231027<strong>Background and Objective:</strong> High-fat diet (HFD) causes disturbances in energy production pathways and increases the possibility of insulin resistance (IR). The purpose of this study was to investigate the effect of high-intensity interval training (HIIT) on metabolic profile associated with IR in male rats fed with HFD and standard diet (SD).<br /><strong>Materials and Methods:</strong> 32 male rats (aged 7-8 weeks) were divided into four equal groups including HIIT+HFD, HIIT+SD, HFD control, and SD control. Then, HIIT (12 weeks, five sessions per week at intensity of 85-90% of maximum oxygen consumption) and diet interventions were applied and finally, the gastrocnemius muscle was removed for tissue examination by gas chromatography-mass spectrometry. One-way analysis of variance, one-way analysis of covariance, and Kruskal-Wallis tests were used for data analysis at a significance level of P < 0.05.<br /><strong>Results</strong><strong>:</strong> The level of citric acid in the gastrocnemius muscle was significantly lower in the HFD control group than in the SD control group. In addition, the level of citric acid in HIIT+SD and HIIT+HFD groups was significantly higher than in the HFD control group. There was no significant difference between the levels of malate, glucose 6-phosphate, ribose, xylose, and ribulose 5-phosphate metabolites among the groups. Also, the body weight in the HIIT+SD and HIIT+HFD groups was significantly lower than the HFD control group.<br /><strong>Conclusion: </strong>HFD limits the aerobic energy provision of skeletal muscles by reducing the level of muscle citric acid as an important intermediate metabolite in the TCA cycle. HIIT can be effectively reduced metabolic disorders caused by HFD by increasing the level of citric acid and reducing the slope of body weight gain.<strong>Background and Objective:</strong> High-fat diet (HFD) causes disturbances in energy production pathways and increases the possibility of insulin resistance (IR). The purpose of this study was to investigate the effect of high-intensity interval training (HIIT) on metabolic profile associated with IR in male rats fed with HFD and standard diet (SD).<br /><strong>Materials and Methods:</strong> 32 male rats (aged 7-8 weeks) were divided into four equal groups including HIIT+HFD, HIIT+SD, HFD control, and SD control. Then, HIIT (12 weeks, five sessions per week at intensity of 85-90% of maximum oxygen consumption) and diet interventions were applied and finally, the gastrocnemius muscle was removed for tissue examination by gas chromatography-mass spectrometry. One-way analysis of variance, one-way analysis of covariance, and Kruskal-Wallis tests were used for data analysis at a significance level of P < 0.05.<br /><strong>Results</strong><strong>:</strong> The level of citric acid in the gastrocnemius muscle was significantly lower in the HFD control group than in the SD control group. In addition, the level of citric acid in HIIT+SD and HIIT+HFD groups was significantly higher than in the HFD control group. There was no significant difference between the levels of malate, glucose 6-phosphate, ribose, xylose, and ribulose 5-phosphate metabolites among the groups. Also, the body weight in the HIIT+SD and HIIT+HFD groups was significantly lower than the HFD control group.<br /><strong>Conclusion: </strong>HFD limits the aerobic energy provision of skeletal muscles by reducing the level of muscle citric acid as an important intermediate metabolite in the TCA cycle. HIIT can be effectively reduced metabolic disorders caused by HFD by increasing the level of citric acid and reducing the slope of body weight gain.https://daneshvarmed.shahed.ac.ir/article_4313_7716d0fc31636914783865d34f6cdfd5.pdfShahed UniversityDaneshvar Medicine2716-972331520240105The effect of 6-week endurance training with the consumption of nettle hydroalcoholic extract on testicular tissue apoptosis in streptozotocin-induced diabetic ratsThe effect of 6-week endurance training with the consumption of nettle hydroalcoholic extract on testicular tissue apoptosis in streptozotocin-induced diabetic rats92103431410.22070/daneshmed.2024.18442.1423FAFatemehFatholahiExercise Physiology Department, Faculty of Humanity Sciences, Lorestan University, Lorestan, Iran0009-0001-0863-2761MasoudRahmatiExercise Physiology Department, Faculty of Humanity Sciences, Lorestan University, Lorestan, Iran0000-0003-4792-027XRahimMirnasouriExercise Physiology Department, Faculty of Humanity Sciences, Lorestan University, Lorestan, Iran0000-0002-9557-2081HadisMehrabi Fard,Exercise Physiology Department, Faculty of Humanity Sciences, Lorestan University, Lorestan, Iran0000-0002-6090-156XJournal Article20231108<strong>Background and Objective:</strong> Diabetes is a metabolic disease that is associated with infertility disorders and testicular tissue apoptosis. The present study was conducted with the aim of investigating the effect of 6 weeks of endurance training along with the consumption of nettle hydroalcoholic extract on the apoptosis of the testicular tissue of diabetic rats.
<strong>Materials and Methods:</strong> 58 male Wistar rats were randomly divided into five groups: "healthy control", "control diabetes", "exercise diabetes", "supplementary diabetes" and "supplementary exercise diabetes". Diabetes was induced by intraperitoneal injection of 45 mg/kg STZ dissolved in fresh citrate buffer. Nettle plant extract was fed to the animals for 6 weeks. The endurance training protocol was performed for 6 weeks and 48 hours after the last training session, the rats were dissected and their testicular tissue was extracted. Tunnel staining method was used to check the apoptosis of testicular tissue. The results of the Kolmogorov Smirnov and Lunn tests were used to check the normality of the data and the homogeneity of the variances. Also, one-way analysis of variance (Tukey's post hoc test) was used to compare groups for the studied variables.
<strong>Results</strong><strong>:</strong> Data analysis showed that apoptosis was significantly reduced in the "exercise diabetes", "supplementary diabetes" and "supplementary exercise diabetes" groups compared to the "control diabetes" group (P = 0.001). Nettle plant extract and endurance exercise in diabetic rats reduced testicular tissue apoptosis.
<strong>Conclusion: </strong>Consumption of nettle hydroalcoholic extract along with endurance exercise can reduce the apoptosis of testicular tissue in diabetic rats. Therefore, it is suggested to have exercise as a non-pharmacological intervention and nettle plant, which is a non-chemical intervention, to reduce the complications caused by diabetes in the field of fertility.<strong>Background and Objective:</strong> Diabetes is a metabolic disease that is associated with infertility disorders and testicular tissue apoptosis. The present study was conducted with the aim of investigating the effect of 6 weeks of endurance training along with the consumption of nettle hydroalcoholic extract on the apoptosis of the testicular tissue of diabetic rats.
<strong>Materials and Methods:</strong> 58 male Wistar rats were randomly divided into five groups: "healthy control", "control diabetes", "exercise diabetes", "supplementary diabetes" and "supplementary exercise diabetes". Diabetes was induced by intraperitoneal injection of 45 mg/kg STZ dissolved in fresh citrate buffer. Nettle plant extract was fed to the animals for 6 weeks. The endurance training protocol was performed for 6 weeks and 48 hours after the last training session, the rats were dissected and their testicular tissue was extracted. Tunnel staining method was used to check the apoptosis of testicular tissue. The results of the Kolmogorov Smirnov and Lunn tests were used to check the normality of the data and the homogeneity of the variances. Also, one-way analysis of variance (Tukey's post hoc test) was used to compare groups for the studied variables.
<strong>Results</strong><strong>:</strong> Data analysis showed that apoptosis was significantly reduced in the "exercise diabetes", "supplementary diabetes" and "supplementary exercise diabetes" groups compared to the "control diabetes" group (P = 0.001). Nettle plant extract and endurance exercise in diabetic rats reduced testicular tissue apoptosis.
<strong>Conclusion: </strong>Consumption of nettle hydroalcoholic extract along with endurance exercise can reduce the apoptosis of testicular tissue in diabetic rats. Therefore, it is suggested to have exercise as a non-pharmacological intervention and nettle plant, which is a non-chemical intervention, to reduce the complications caused by diabetes in the field of fertility.https://daneshvarmed.shahed.ac.ir/article_4314_2bf7e9e8f3f3bce1ac5212f22414aa57.pdf