Shahed UniversityDaneshvar Medicine2716-972320220120621Personality characteristics and stress in peptic ulcer patients and healthy individuals: a comparative study considering sex differencesPersonality characteristics and stress in peptic ulcer patients and healthy individuals: a comparative study considering sex differences1515FANaghiRahnama NehzamiSajjadRezaeiFarhadJomehriHasanAhadiJournal Article20200926 Background
and Objective: Personality is a principal factor in present relationship
between stress and health. Thus, it is not far from expectations that
psychosomatic diseases are affected by personality characteristics and coping
style with life stressor events. The aim of the present research was a
comparison between personality characteristic and stress
in patients with peptic ulcer disease
(PUD) and healthy individuals, considering their sex differences. Materials and
Methods: This
research was a matched case-control study in which 60 patients, referring to 8
personal clinics with a diagnosis or a history of peptic ulcer based on the viewpoint
of a therapeutic physician were compared with 60 healthy individuals as the control
group, regarding personality characteristics and the level of experienced
stress. Males and females had the same number in both groups (30 males and 30
females). Research tools included Holmes and Rahe Social Readjustment Rating
Scale to measure the level of daily life stressors and NEO-Five Factor
Inventory (NEO-FFI) to evaluate 5 personality factors of Neuroticism (N),
Extroversion (E), Openness (O), Agreeableness (A), and Conscientiousness(C). The
research data were analyzed by SPSS (Version 16.0) and multivariate analysis of
variance (MANOVA) and regression (by enter method) tests. Results: The level of stress in patients with PUD was
higher than that of control group. Considering the personality characteristics,
the patient's group had remarkably higher scores in (N), (E), and (O) and in
this circumstance had significant role the interactional effect of gender with disease
on levels of stress (p < 0.05). Regression analysis revealed that only
personality characteristic of Neuroticism (N) significantly predicted the
experienced stress (F=12.206 p < 0.01). Conclusion:
Altogether, it is concluded that reducing the stress sources to the least
possible level in PUD patients seems necessary. Moreover, targeting the
characteristic of Neuroticism (N) is a key factor. From the personality
perspective, women with excessive level of openness (O) are at risk of PUD. Background
and Objective: Personality is a principal factor in present relationship
between stress and health. Thus, it is not far from expectations that
psychosomatic diseases are affected by personality characteristics and coping
style with life stressor events. The aim of the present research was a
comparison between personality characteristic and stress
in patients with peptic ulcer disease
(PUD) and healthy individuals, considering their sex differences. Materials and
Methods: This
research was a matched case-control study in which 60 patients, referring to 8
personal clinics with a diagnosis or a history of peptic ulcer based on the viewpoint
of a therapeutic physician were compared with 60 healthy individuals as the control
group, regarding personality characteristics and the level of experienced
stress. Males and females had the same number in both groups (30 males and 30
females). Research tools included Holmes and Rahe Social Readjustment Rating
Scale to measure the level of daily life stressors and NEO-Five Factor
Inventory (NEO-FFI) to evaluate 5 personality factors of Neuroticism (N),
Extroversion (E), Openness (O), Agreeableness (A), and Conscientiousness(C). The
research data were analyzed by SPSS (Version 16.0) and multivariate analysis of
variance (MANOVA) and regression (by enter method) tests. Results: The level of stress in patients with PUD was
higher than that of control group. Considering the personality characteristics,
the patient's group had remarkably higher scores in (N), (E), and (O) and in
this circumstance had significant role the interactional effect of gender with disease
on levels of stress (p < 0.05). Regression analysis revealed that only
personality characteristic of Neuroticism (N) significantly predicted the
experienced stress (F=12.206 p < 0.01). Conclusion:
Altogether, it is concluded that reducing the stress sources to the least
possible level in PUD patients seems necessary. Moreover, targeting the
characteristic of Neuroticism (N) is a key factor. From the personality
perspective, women with excessive level of openness (O) are at risk of PUD. https://daneshvarmed.shahed.ac.ir/article_1515_1cea2da6154982474af8d0762645ac7f.pdfShahed UniversityDaneshvar Medicine2716-972320220120621Comparing the efffect of vincristine on proliferating normal lymphocytes and BCL1 cell lineComparing the efffect of vincristine on proliferating normal lymphocytes and BCL1 cell line1101516FAMohaddeseShahhosseiniMohaddeseShahhosseiniMohaddeseShahhosseiniSussanKabudanianArdestaniRoyaYaraee0000-0002-6133-0017Journal Article20200926 Background and Objective: Proliferation and apoptosis of
lymphocytes are essential parts of the immune system. Most anti-cancer
chemotherapy drugs such as vincristine target cell cycle and induce apoptosis
in cancer cells. In other words,
dividing cells undergo more apoptosis, which may also include the normal
proliferating lymphocytes responsive to malignancies as well. Materials and Methods: In this study, the effect of different concentration of
vincristine at three different time periods on resting and proliferating spleen
lymphocytes were evaluated
and compared with the effect of the drug on mouse lymphoma cell line BCL1. The cytotoxicity of vincristine
was determined by MTT assay and IC50 was calculated for all periods. The cells
were also stained with double staining acridine orange and ethidium bromide and
were observed with fluorescence microscope and the percentage of apoptotic
cells were determined. Results: MTT
results showed that vincristine at concentrations of 10 and 20 μg/ml caused
cell death in both resting and proliferating lymphocytes, but
concentrations Background and Objective: Proliferation and apoptosis of
lymphocytes are essential parts of the immune system. Most anti-cancer
chemotherapy drugs such as vincristine target cell cycle and induce apoptosis
in cancer cells. In other words,
dividing cells undergo more apoptosis, which may also include the normal
proliferating lymphocytes responsive to malignancies as well. Materials and Methods: In this study, the effect of different concentration of
vincristine at three different time periods on resting and proliferating spleen
lymphocytes were evaluated
and compared with the effect of the drug on mouse lymphoma cell line BCL1. The cytotoxicity of vincristine
was determined by MTT assay and IC50 was calculated for all periods. The cells
were also stained with double staining acridine orange and ethidium bromide and
were observed with fluorescence microscope and the percentage of apoptotic
cells were determined. Results: MTT
results showed that vincristine at concentrations of 10 and 20 μg/ml caused
cell death in both resting and proliferating lymphocytes, but
concentrationshttps://daneshvarmed.shahed.ac.ir/article_1516_7cf8f73e6c8ebf406c138360b3f6c506.pdfShahed UniversityDaneshvar Medicine2716-972320220120621Exclusive breast-feeding weaning pattern and its determinant factors in Fars province in 2010.Exclusive breast-feeding weaning pattern and its determinant factors in Fars province in 2010.11221517FAAlirezaMirahmadizadehParvinZareFaribaMoradiMehrabSayadiElhamHesamiMohsenMoghadamiJournal Article20200926 Background and Objective: In addition to the importance of exclusive
breast-feeding (EBF) in the first six months of life, its weaning is also
important. This study aimed to determine the pattern of EBF weaning and its
associated determinants in Fars province (southern Iran). Materials and Methods: In this historical cohort study, records of 751
infants 6 to 12 months (urban and rural) were selected as multi-random sampling
method. Other necessary data were also collected through questionnaires and
interviews. In addition to descriptive statistics using statistical tests and
survival analysis, the effect of demographic, cultural and labor-related
variables on exclusive EBF weaning was also studied. Results: Averaged time of EBF weaning was 4.64±1.68 months
and 78.4% of infants have exclusive EBF for 4 months and 50.7% for 6 months.
There were not any association between race, official education, sex and birth
rank with time of EBF weaning, (p>0.05). Most children (68.5%) began their
supplementary food with porridge. Conclusion: Duration of EBF in this study was more than
other studies. Lack of significant association between demographic variables,
cultural and labor-related variables with duration of EBF, indicated acceptable
public health education and lack of wrong beliefs among parents. To promote
breast feeding indices, education of parents and breast feeding clinic
establishment are necessary. Background and Objective: In addition to the importance of exclusive
breast-feeding (EBF) in the first six months of life, its weaning is also
important. This study aimed to determine the pattern of EBF weaning and its
associated determinants in Fars province (southern Iran). Materials and Methods: In this historical cohort study, records of 751
infants 6 to 12 months (urban and rural) were selected as multi-random sampling
method. Other necessary data were also collected through questionnaires and
interviews. In addition to descriptive statistics using statistical tests and
survival analysis, the effect of demographic, cultural and labor-related
variables on exclusive EBF weaning was also studied. Results: Averaged time of EBF weaning was 4.64±1.68 months
and 78.4% of infants have exclusive EBF for 4 months and 50.7% for 6 months.
There were not any association between race, official education, sex and birth
rank with time of EBF weaning, (p>0.05). Most children (68.5%) began their
supplementary food with porridge. Conclusion: Duration of EBF in this study was more than
other studies. Lack of significant association between demographic variables,
cultural and labor-related variables with duration of EBF, indicated acceptable
public health education and lack of wrong beliefs among parents. To promote
breast feeding indices, education of parents and breast feeding clinic
establishment are necessary. https://daneshvarmed.shahed.ac.ir/article_1517_6d86d2175a0a07241aa1fe1f16f11182.pdfShahed UniversityDaneshvar Medicine2716-972320220120621Semi-parametric Cox regression for factors affecting hospitalization lengthSemi-parametric Cox regression for factors affecting hospitalization length23301518FAMahmoodrezaGohariNasimVahabiZeinabMoghadamifardJournal Article20200926 Background and Objective: Length of stay (LOS) is one of
the most important indexes for performance evaluation of hospitals and their
manager. With respect to the importance of this
index, we determined the factors affecting LOS. Materials and Methods: This was an analytical study. The
under study population included patients which died in Hasheminejad hospital in
2010 and 935 patients using multi-stage cluster sampling method were selected.
Variables, LOS, age, insurance and ICD10 code were gathered from patients’
files. Factors associated to LOS were analyzed using R software and
semi-parameter Cox regression model. Results: It was found out that 62.5% (585) of
patients was women and most of them had an age larger than 50 years. Mean age (±SD)
of patients was 50/02 (±19.07). In addition, 56% (586) of patients had
Tamin-Ejtemaee insurance and 19.6% (185) had stayed without insurance or with
complementary insurance. Mean LOS (±SD) of patients was 12.77 (±11.131) and LOS
of men was more than women with a significant difference (p=0.005). Median of
LOS was 14.2. The results of Cox regression for the variables age and sex was
significant (p < .001) and insurance had not a significant effect on LOS. Conclusion: Two important features of LOS data are
non-normality and presence of censorship, so using classic models for such data
is not useful and this causes estimations with low precision. Because of these
two features and for having more precise estimation, using survival analysis is
suggested for such data. Background and Objective: Length of stay (LOS) is one of
the most important indexes for performance evaluation of hospitals and their
manager. With respect to the importance of this
index, we determined the factors affecting LOS. Materials and Methods: This was an analytical study. The
under study population included patients which died in Hasheminejad hospital in
2010 and 935 patients using multi-stage cluster sampling method were selected.
Variables, LOS, age, insurance and ICD10 code were gathered from patients’
files. Factors associated to LOS were analyzed using R software and
semi-parameter Cox regression model. Results: It was found out that 62.5% (585) of
patients was women and most of them had an age larger than 50 years. Mean age (±SD)
of patients was 50/02 (±19.07). In addition, 56% (586) of patients had
Tamin-Ejtemaee insurance and 19.6% (185) had stayed without insurance or with
complementary insurance. Mean LOS (±SD) of patients was 12.77 (±11.131) and LOS
of men was more than women with a significant difference (p=0.005). Median of
LOS was 14.2. The results of Cox regression for the variables age and sex was
significant (p < .001) and insurance had not a significant effect on LOS. Conclusion: Two important features of LOS data are
non-normality and presence of censorship, so using classic models for such data
is not useful and this causes estimations with low precision. Because of these
two features and for having more precise estimation, using survival analysis is
suggested for such data. https://daneshvarmed.shahed.ac.ir/article_1518_f0a5facc02d56befaa743730a3585eeb.pdfShahed UniversityDaneshvar Medicine2716-972320220120621Evaluation of the combination of financing and providing services in social health insurance system in IranEvaluation of the combination of financing and providing services in social health insurance system in Iran31381519FAMahmoudKazemianMohammadMeskarpour-AmiriJournal Article20200926 Background and Objective: The aim of the present study was
assessment of the status of combination of financing and providing services in
Medical Service Insurance Organization (MSIO) as unique provider. Materials and Methods: Present study was an applied study
that was performed in a descriptive-analytical and statistical manner. The
research population consisted of all Medical Service Insurance Organization’s
(MSIO’s) insured and total services delivered to them. In this study, the time
comparison of correlation coefficient in estimation regression of exponential
function in two times 2005 and 2009 was used. Comparison of changes in
residuals distribution was used for determination of changes in province’s
situation. Finally, the Eviews econometrics software was used for estimation
regression. Results: This study showed that in provinces of country,
the effect of per capital care received by insured on per capital cost pay by
insurance organization significantly reduced from 0.25 in 2005 to 0.14 in 2009
(p < 0.05). Also, with regard to changes in relative situation of provinces in
these periods of time, 8 provinces have worse situation because did not tend to
combination of per capital cost and per capital refers as unique production and
in situation of 16 provinces did not take place noticeable changes. Conclusion: It seems that after about two decades from
policy of developing social health insurance (SHI) in Iran, the financing and
services delivery system through role of social health insurances could not be
able for making it possible to combine financing and services delivery as
unique production for insurers. Background and Objective: The aim of the present study was
assessment of the status of combination of financing and providing services in
Medical Service Insurance Organization (MSIO) as unique provider. Materials and Methods: Present study was an applied study
that was performed in a descriptive-analytical and statistical manner. The
research population consisted of all Medical Service Insurance Organization’s
(MSIO’s) insured and total services delivered to them. In this study, the time
comparison of correlation coefficient in estimation regression of exponential
function in two times 2005 and 2009 was used. Comparison of changes in
residuals distribution was used for determination of changes in province’s
situation. Finally, the Eviews econometrics software was used for estimation
regression. Results: This study showed that in provinces of country,
the effect of per capital care received by insured on per capital cost pay by
insurance organization significantly reduced from 0.25 in 2005 to 0.14 in 2009
(p < 0.05). Also, with regard to changes in relative situation of provinces in
these periods of time, 8 provinces have worse situation because did not tend to
combination of per capital cost and per capital refers as unique production and
in situation of 16 provinces did not take place noticeable changes. Conclusion: It seems that after about two decades from
policy of developing social health insurance (SHI) in Iran, the financing and
services delivery system through role of social health insurances could not be
able for making it possible to combine financing and services delivery as
unique production for insurers. https://daneshvarmed.shahed.ac.ir/article_1519_73ba431dd971128666afcc42cf4d096a.pdfShahed UniversityDaneshvar Medicine2716-972320220120621Assesment of the relationship between empowerment and reproductive behaviorAssesment of the relationship between empowerment and reproductive behavior39461520FASoheilaFroozanfarFereshtehMajlessiAbbasRahimi ForroshaniAbolghasemPour RezaJournal Article20200926 Background and Objective: The1994
International Conference on Population and Development (ICPD) specifically
identified “empowering women” as an essential ingredient for achieving
desirable reproductive health and population outcomes. This study was done to
determine the relation between women empowerment and reproductive behaviors. Materials and Methods: An analytical descriptive survey was
carried out for 629 married women aged 15-50 in western
Tehran health centers. Data gathering was through the questionnaires of
demographic, sexual, reproductive history and socio-economical information.
Randomized sampling was done by interview. Data was analyzed in spss16.
Descriptive statistics was used to describe the samples and X2 test
for determination of relation between qualitative variables. Logistic
regression analysis was used for assessing the power of independent variables
that had a significant effect on reproductive behavior. Results: Findings showed that 29.6% of cases were in good
level of reproductive behavior and 20.8% of them were in high level of
empowerment. Overall, women empowerment and reproductive behavior were in the
mid-level, but an improvement was seen in the reproductive behavior with
improvement of the women empowerment. There was also a significant relationship
between reproductive behavior and women age (p=0.01), literacy (p=0.002),
empowerment (p=0.007) and a negative significant relationship with the number
of children (p=0.002) and marital duration (p=0.001). There was no significant
relationship between empowerment and reproductive behavior in logestic
regression because of correlation between variables such as age and education
that inserted in logestic regression simultaneously. Conclusion: Reproductive behavior was considerably affected
by education and socio-economical factors that are determinant factors of
empowerment, hence great attention to women empowerment issue is necessary for
improvement of human development indices and reproductive behaviors. Background and Objective: The1994
International Conference on Population and Development (ICPD) specifically
identified “empowering women” as an essential ingredient for achieving
desirable reproductive health and population outcomes. This study was done to
determine the relation between women empowerment and reproductive behaviors. Materials and Methods: An analytical descriptive survey was
carried out for 629 married women aged 15-50 in western
Tehran health centers. Data gathering was through the questionnaires of
demographic, sexual, reproductive history and socio-economical information.
Randomized sampling was done by interview. Data was analyzed in spss16.
Descriptive statistics was used to describe the samples and X2 test
for determination of relation between qualitative variables. Logistic
regression analysis was used for assessing the power of independent variables
that had a significant effect on reproductive behavior. Results: Findings showed that 29.6% of cases were in good
level of reproductive behavior and 20.8% of them were in high level of
empowerment. Overall, women empowerment and reproductive behavior were in the
mid-level, but an improvement was seen in the reproductive behavior with
improvement of the women empowerment. There was also a significant relationship
between reproductive behavior and women age (p=0.01), literacy (p=0.002),
empowerment (p=0.007) and a negative significant relationship with the number
of children (p=0.002) and marital duration (p=0.001). There was no significant
relationship between empowerment and reproductive behavior in logestic
regression because of correlation between variables such as age and education
that inserted in logestic regression simultaneously. Conclusion: Reproductive behavior was considerably affected
by education and socio-economical factors that are determinant factors of
empowerment, hence great attention to women empowerment issue is necessary for
improvement of human development indices and reproductive behaviors. https://daneshvarmed.shahed.ac.ir/article_1520_52f35dc288a012456cb1fd41829dc736.pdfShahed UniversityDaneshvar Medicine2716-972320220120621The effect of oral feeding of Withania somnifera L. alcoholic extract on intracerebroventricular streptozotocin-induced cognitive deficits in male ratThe effect of oral feeding of Withania somnifera L. alcoholic extract on intracerebroventricular streptozotocin-induced cognitive deficits in male rat47561521FAMohsenKhalili1111-2222-3333-444XMohammadrezaVaez MahdaviJournal Article20200926 Background and Objective: Alzheimer’s disease is a
prevalent central nervous system disease with no definitive treatment till now.
So, in the present study the effect of an important herbal medicine, withania
somnifera (Ws), was investigated on progression of Alzheimer’s
disease. Materials and Methods: We used intracerebroventricular
streptozotocin (STZ) injection as a model for Alzheimer's disease. Learning and
memory performance was assessed using passive avoidance paradigm and for
spatial cognition evaluation, Y maze task was used. The studied animals were
divided into control, control+Ws, STZ and STZ+Ws. The STZ-injected rats
received Ws extract (p.o) one day pre-surgery for three weeks and were then
subjected to Y maze and passive avoidance learning and memory tests. Finally,
the acquired data were subjected to proper statistical analysis. Results: It was found out that treatment with the extract
(200 mg/kg) could markedly increase the step through latency (STL) time (43.5 ±
3.48) as an index of recall in comparison to STZ-injected rats (33.1 ± 3.1).
Also, alternation behavior as spatial memory index was augmented from 45.21 ±
4.81 in control animals to 67.68 ± 5.21 and 63.26 ± 6.27 in 100 and 200 mg/kg
treated animal groups, respectively. Conclusion: Therefore, chronic treatment with Ws extract
could antagonize memory deficits induced by STZ-ICV injection in rats. Perhaps,
this plant could be a potential factor in the treatment of neurodegenerative
diseases such as Alzheimer's disease (AD). Background and Objective: Alzheimer’s disease is a
prevalent central nervous system disease with no definitive treatment till now.
So, in the present study the effect of an important herbal medicine, withania
somnifera (Ws), was investigated on progression of Alzheimer’s
disease. Materials and Methods: We used intracerebroventricular
streptozotocin (STZ) injection as a model for Alzheimer's disease. Learning and
memory performance was assessed using passive avoidance paradigm and for
spatial cognition evaluation, Y maze task was used. The studied animals were
divided into control, control+Ws, STZ and STZ+Ws. The STZ-injected rats
received Ws extract (p.o) one day pre-surgery for three weeks and were then
subjected to Y maze and passive avoidance learning and memory tests. Finally,
the acquired data were subjected to proper statistical analysis. Results: It was found out that treatment with the extract
(200 mg/kg) could markedly increase the step through latency (STL) time (43.5 ±
3.48) as an index of recall in comparison to STZ-injected rats (33.1 ± 3.1).
Also, alternation behavior as spatial memory index was augmented from 45.21 ±
4.81 in control animals to 67.68 ± 5.21 and 63.26 ± 6.27 in 100 and 200 mg/kg
treated animal groups, respectively. Conclusion: Therefore, chronic treatment with Ws extract
could antagonize memory deficits induced by STZ-ICV injection in rats. Perhaps,
this plant could be a potential factor in the treatment of neurodegenerative
diseases such as Alzheimer's disease (AD). https://daneshvarmed.shahed.ac.ir/article_1521_0046ed2fcb866ae45de885b43e0f25d2.pdfShahed UniversityDaneshvar Medicine2716-972320220120621The effect of five-week daily aerobic exercise training and ten-week every other day aerobic training on some markers of women with type 2 diabetesThe effect of five-week daily aerobic exercise training and ten-week every other day aerobic training on some markers of women with type 2 diabetes71781522FAMohammad RezaHamediniaMitraKhademosharieMitraKhademosharieTayebeAmiri-ParsaMarzie SadatAzarniveMarzie SadatAzarniveMitraKhademosharieMarzie SadatAzarniveMehdiHedayatiJournal Article20200926 Background and Objective: Concerning performing effective
exercise (intensity, duration, type and number of sessions in a week), there
are different recommendations for diabetic patients. The purpose of this study
was to compare the effect of five weeks of daily aerobic exercise training and
every other day aerobic training for ten weeks in the women with type 2
diabetes. Materials and Methods: Thirty six women with type 2
diabetes were randomly divided into three groups: daily 5-week aerobic exercise
training group (12 women), every other day 10-week aerobic exercise training
group (12 women) and control group. Aerobic training was conducted with an
intensity of 60%-70% of maximum heart rate for 30-60 min per session.
Measurements and blood sampling in the beginning of research protocol, after 5
weeks and 10 weeks after implementation of research protocols were conducted.
For analyzing the data, ANOVA with replications and One-Way ANOVA were applied.
Results: Five-week daily aerobic exercise training did not
have a significant effect on weight (p=0.5), body fat index (p=0.54), BMI
(p=0.65), adiponectin (p=0.91), and insulin (p=0.06). Ten week every other day
aerobic exercise training also did not have a significant effect on weight
(p=0.35), body fat index (p=0.30), BMI (p=0.27), adiponectin (p=0.51), insulin
(p=0.51), HbA1C (p=0.34), glucose (P=0.94) and the insulin resistance index
(p=0.53). There was not also a significant difference between these two
protocols of aerobic training. Conclusion: Five week daily aerobic training or 10 week
every other day aerobic training do not have a significant effect in diabetic
patients and for effective aerobic training, these patients should perform
these trainings within a prolonged duration. Background and Objective: Concerning performing effective
exercise (intensity, duration, type and number of sessions in a week), there
are different recommendations for diabetic patients. The purpose of this study
was to compare the effect of five weeks of daily aerobic exercise training and
every other day aerobic training for ten weeks in the women with type 2
diabetes. Materials and Methods: Thirty six women with type 2
diabetes were randomly divided into three groups: daily 5-week aerobic exercise
training group (12 women), every other day 10-week aerobic exercise training
group (12 women) and control group. Aerobic training was conducted with an
intensity of 60%-70% of maximum heart rate for 30-60 min per session.
Measurements and blood sampling in the beginning of research protocol, after 5
weeks and 10 weeks after implementation of research protocols were conducted.
For analyzing the data, ANOVA with replications and One-Way ANOVA were applied.
Results: Five-week daily aerobic exercise training did not
have a significant effect on weight (p=0.5), body fat index (p=0.54), BMI
(p=0.65), adiponectin (p=0.91), and insulin (p=0.06). Ten week every other day
aerobic exercise training also did not have a significant effect on weight
(p=0.35), body fat index (p=0.30), BMI (p=0.27), adiponectin (p=0.51), insulin
(p=0.51), HbA1C (p=0.34), glucose (P=0.94) and the insulin resistance index
(p=0.53). There was not also a significant difference between these two
protocols of aerobic training. Conclusion: Five week daily aerobic training or 10 week
every other day aerobic training do not have a significant effect in diabetic
patients and for effective aerobic training, these patients should perform
these trainings within a prolonged duration. https://daneshvarmed.shahed.ac.ir/article_1522_1c1de986c216450141ed115b251f34fa.pdfShahed UniversityDaneshvar Medicine2716-972320220120621Cytotoxicity of nano-hydroxyapatite on gingiva-derived fibroblast cell line (HGF2): An in vitro studyCytotoxicity of nano-hydroxyapatite on gingiva-derived fibroblast cell line (HGF2): An in vitro study79861523FANoushinJalayer NaderiRoyaYaraeeDavoudJamaliMohammad BagherRezvaniJournal Article20200926 Background and Objective: With expansion of nanotechnology,
nano-hydroxyapatite particles are used in medicine and dentistry fields. The
biological effects of these particles are not completely defined. The aim of
this study was to determine the cytotoxicity of nano-hydroxyapatite on
gingiva-derived fibroblasts. Materials and Methods: Various concentrations of
nano-hydroxyapatite from 2 to 0.002 mg/ml were
treated on gingiva-derived fibroblast cell line (HGF2) in 24, 48 and 72 hours.
MTT and LDH methods were used for evaluation of cytotoxic effect. Results: MTT method: doses higher than 5 and 2 mg/ml in 24
hours, doses higher than 0.2, 2 and 5 mg/ml and lower than 0.01, 0.02, 0.05,
0.002 and 0.005 in 48 hours and doses higher than 0.5,
1, 2, and 5 mg/ml and doses lower than 0, 0.01, 0.02, 0.002 and 0.005 mg/ml in
72 hours caused significant decreases in OD and absorbance of fibroblasts. LDH
method: significant increase of LDH release noticed with 5 mg/ml dose in 24
hours, 1, 2, 5 mg/ml doses in 48 hours and 5 mg/ml dose in 72 hours. LDH
release decreased with 0.02 mg/ml and 0.01 mg/ml in 24 and 48 hours,
respectively. The IC50 was about
6 mg/ml in 24, 48 and 72 hours. Conclusion: In 24 hours and after that, high
doses of nano-hydroxyapatite have cytotoxic effect on gingival-derived
fibroblasts. Background and Objective: With expansion of nanotechnology,
nano-hydroxyapatite particles are used in medicine and dentistry fields. The
biological effects of these particles are not completely defined. The aim of
this study was to determine the cytotoxicity of nano-hydroxyapatite on
gingiva-derived fibroblasts. Materials and Methods: Various concentrations of
nano-hydroxyapatite from 2 to 0.002 mg/ml were
treated on gingiva-derived fibroblast cell line (HGF2) in 24, 48 and 72 hours.
MTT and LDH methods were used for evaluation of cytotoxic effect. Results: MTT method: doses higher than 5 and 2 mg/ml in 24
hours, doses higher than 0.2, 2 and 5 mg/ml and lower than 0.01, 0.02, 0.05,
0.002 and 0.005 in 48 hours and doses higher than 0.5,
1, 2, and 5 mg/ml and doses lower than 0, 0.01, 0.02, 0.002 and 0.005 mg/ml in
72 hours caused significant decreases in OD and absorbance of fibroblasts. LDH
method: significant increase of LDH release noticed with 5 mg/ml dose in 24
hours, 1, 2, 5 mg/ml doses in 48 hours and 5 mg/ml dose in 72 hours. LDH
release decreased with 0.02 mg/ml and 0.01 mg/ml in 24 and 48 hours,
respectively. The IC50 was about
6 mg/ml in 24, 48 and 72 hours. Conclusion: In 24 hours and after that, high
doses of nano-hydroxyapatite have cytotoxic effect on gingival-derived
fibroblasts. https://daneshvarmed.shahed.ac.ir/article_1523_397dfe8f6d1e04db49a21d22c2a33bd5.pdf