Shahed University Daneshvar Medicine 2716-9723 29 3 2021 08 23 Survey on the interaction effect of low doses of ketamine on morphine-induced polycystic ovary syndrome in rat Survey on the interaction effect of low doses of ketamine on morphine-induced polycystic ovary syndrome in rat 29 41 3343 10.22070/daneshmed.2021.14147.1055 FA Masoumeh Bakhtiary Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran 0000-0001-9756-9284 Manizheh Karami Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran 0000-0003-0353-7359 Mohammadreza Jalali Nadoushan Department of Pathology, Faculty of Medicine, Shahed University, Tehran, Iran 0000-0002-8817-2215 Fatemeh BAbaei Sosahab Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran Journal Article 2021 02 13 <strong>Background and Objective:</strong> Morphine can induce PCOS (Polycystic ovary syndrome) probably by changing glutamate level at the hypothalamic nuclei. This study used low doses of ketamine to survey the interaction of ketamine with morphine-induced PCOS. <br /><strong>Materials and Methods:</strong> 48 female Wistar rats were selected as virgins in the weight range of 220-250 g, and categorized randomly into eight groups. The first group received morphine (5 mg/kg) intraperitoneally. The second to fourth groups received ketamine (1, 2, and 4 mg/kg). In groups 5 to 7, the ketamine (1, 2, and 4 mg/kg) was pre-injected to morphine (5 mg/kg) over a period of 20 min. Control group received only saline (1 ml/kg). After 48 h, the blood and serum specimens were provided to record hormone levels, and the animals’ ovaries and uteri were biometrically examined and stained by H & E. Statistical analysis was performed by analysis of variance (ANOVA) under α=0.05. <br /><strong>Results:</strong> The morphine as well as single ketamine induced the PCOS. However, taking ketamine prior to morphine caused a meaningful reduction in the number of cysts in the ovary. There was also a relative increase in uterine diameter in the group receiving morphine, which was stopped due to ketamine pre-treatment. These effects showed correlation with decreased levels of FSH, and change in estrogen, and prolactin amounts. <br /><strong>Conclusion</strong>: The interference effect of ketamine with morphine is completely dependent on changes in gonadotropin level. <strong>Background and Objective:</strong> Morphine can induce PCOS (Polycystic ovary syndrome) probably by changing glutamate level at the hypothalamic nuclei. This study used low doses of ketamine to survey the interaction of ketamine with morphine-induced PCOS. <br /><strong>Materials and Methods:</strong> 48 female Wistar rats were selected as virgins in the weight range of 220-250 g, and categorized randomly into eight groups. The first group received morphine (5 mg/kg) intraperitoneally. The second to fourth groups received ketamine (1, 2, and 4 mg/kg). In groups 5 to 7, the ketamine (1, 2, and 4 mg/kg) was pre-injected to morphine (5 mg/kg) over a period of 20 min. Control group received only saline (1 ml/kg). After 48 h, the blood and serum specimens were provided to record hormone levels, and the animals’ ovaries and uteri were biometrically examined and stained by H & E. Statistical analysis was performed by analysis of variance (ANOVA) under α=0.05. <br /><strong>Results:</strong> The morphine as well as single ketamine induced the PCOS. However, taking ketamine prior to morphine caused a meaningful reduction in the number of cysts in the ovary. There was also a relative increase in uterine diameter in the group receiving morphine, which was stopped due to ketamine pre-treatment. These effects showed correlation with decreased levels of FSH, and change in estrogen, and prolactin amounts. <br /><strong>Conclusion</strong>: The interference effect of ketamine with morphine is completely dependent on changes in gonadotropin level. https://daneshvarmed.shahed.ac.ir/article_3343_1d2240b45c45661d4562284e8bbabb6b.pdf