Shahed University Daneshvar Medicine 2716-9723 20 5 2012 12 21 Study of biocompatibility of electrospun chitosan-based nanofiber co-cultured with bone marrow stromal cells Study of biocompatibility of electrospun chitosan-based nanofiber co-cultured with bone marrow stromal cells 1 8 3085 FA Asgar Emamgholi Neuroscience Research Center- Baqiyatallah University of Medical Sciences - Tehran Gholam Reza Kaka Neuroscience Research Center- Baqiyatallah University of Medical Sciences - Tehran Minoo Sadri Department of Biochemistry and Biophysics, Education and Research Center of Science and Biotechnology, Malek Ashtar University- Tehran Seyed Homayoon Sadraie Department of Anatomy- Baqiyatallah University of Medical Sciences Department of Anatomy- Tehran Davood Zolfaghari Nanobiotechnology Research Center- Baqiyatallah University of Medical Sciences – Tehran Zahra Borbor Neuroscience Research Center -Baqiyatallah University of Medical Sciences - Tehran Journal Article 2012 11 20 Background and Objective: Several studies have been performedto achieve a scaffold for growing stem cells. The purpose of the study was to provide a biodegradable scaffold of chitosan - poly ethylene oxide (PEO) with the ability for growing, proliferation, un-differentiation and apoptosis of bone marrow stromal cells (BMSCs). Materials and Methods: First, formation of chitosan-PEO nanofibers composed of 90 to 10 and 80 to 20 per electro technique were studied by scanning electron microscope (SEM). These scaffolds were located on 1% gelatin in 24-well plates and were then steriled. Femoral BMSCs of rats were cultured on scaffolds after two passages from the house empty plate as controls. BMSCs proliferation, differentiation and apoptosis were studied in days II, IV and VI. Results: The results showed that the morphology of cells was maintained on scaffolds similarto controls. The rate of cell proliferation on the scaffold on consecutive days increased in cultured cells of control group but the differences were not significant. The results also showed that at the end of the six days, BMSCs differentiation and the percentage of cell death on the scaffold were similar with cultured cells in control group. Conclusion: Proliferation, un-differentiation and no apoptosis of BMSCs on biodegradable chitosan-PEO nanofiber are obtained as a model that can be used in tissue engineering and cell therapy. Background and Objective: Several studies have been performedto achieve a scaffold for growing stem cells. The purpose of the study was to provide a biodegradable scaffold of chitosan - poly ethylene oxide (PEO) with the ability for growing, proliferation, un-differentiation and apoptosis of bone marrow stromal cells (BMSCs). Materials and Methods: First, formation of chitosan-PEO nanofibers composed of 90 to 10 and 80 to 20 per electro technique were studied by scanning electron microscope (SEM). These scaffolds were located on 1% gelatin in 24-well plates and were then steriled. Femoral BMSCs of rats were cultured on scaffolds after two passages from the house empty plate as controls. BMSCs proliferation, differentiation and apoptosis were studied in days II, IV and VI. Results: The results showed that the morphology of cells was maintained on scaffolds similarto controls. The rate of cell proliferation on the scaffold on consecutive days increased in cultured cells of control group but the differences were not significant. The results also showed that at the end of the six days, BMSCs differentiation and the percentage of cell death on the scaffold were similar with cultured cells in control group. Conclusion: Proliferation, un-differentiation and no apoptosis of BMSCs on biodegradable chitosan-PEO nanofiber are obtained as a model that can be used in tissue engineering and cell therapy.

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