Volume 16, Number 79 (3-2009)                   daneshvarmed 2009, 16(79): 51-56 | Back to browse issues page


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alizadeh A, tarihi T, dashtnavard H. The Influence of Lithium Chloride on Induction of Bone Marrow Stromal Cells into Neuronal Phenotype. daneshvarmed. 2009; 16 (79) :51-56
URL: http://daneshvarmed.shahed.ac.ir/article-1-83-en.html

1. M.Sc. in Anatomy
Abstract:   (6321 Views)

  Background & Objective : Bone marrow stromal cells (BMSCs) are a kind of stem cells with high pluripotency. The BMSCs can differentiate into mesodermal and non mesodermal cells. Because of availability of them, they are a suitable source of adult stem cells for cell therapy. Some inducers were used to differentiate stem cells into neural phenotype, such: retinoic acid, dimethyl sulfoxide, deprenil & et al are cytotoxic compound. Lithium is a mood stabilizer drug that has supportive effect on neuronal cells. Our goal in this study is use of lithium chloride as a safe inducer for induction of BMSCs into neuronal phenotype.

  Materials & Methods: The BMSCs were isolated from bone marrow of female rat’s femurs and tibias by aspiration and culturing. After 4 passages they were induced by lithium chloride (0.5mM) for 24 hours. Then they evaluated with microscopic and immunocytochemistry methods. In immunocytochemistry, anti-neurofilament 160, 68 and 200 KD and anti-synaptophysin antibody were used to characterize the neuronal phenotype.

  Results: The results indicated that lithium chloride (0.5mM) can induced the BMSCs into neuronal phenotype after 24 hours. The percentage of fibronectin immunoreactive cells before induction was 95% and the percentage of neural markers immunoreactive cells after induction were 89.6% for neurofilament (NF) 68, 92% for NF-160, 93.2% for NF-200 and 70.2% for synaptophysin.

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Type of Study: Research |

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