Volume 19, Number 95 (11-2011)                   daneshvarmed 2011, 19(95): 65-78 | Back to browse issues page


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Tehranian N, Sepehri H, Biramijamal F, Mehdipour P, Hossein-Nezhad A, Sarrafnejad A, et al . The effect of modified citrus pectin (MCP) on viability, morphology, and cell cycle arrest of DU-145 and LNCaP prostate cancer cell lines. daneshvarmed. 2011; 19 (95) :65-78
URL: http://daneshvarmed.shahed.ac.ir/article-1-395-en.html

Professor Animal Biology Department, School of Biology, Faculty of Sciences, Tehran University
Abstract:   (8993 Views)

Background and Objective: This study was conducted to evaluate the effect of PectaSol on modification of Doxorubicin (Dox) cytotoxicity and apoptosis and cell cycle in prostate cancer cell lines (DU-145 & LNCaP)                                                        

 

Materials and Methods: Human prostate carcinoma DU-145 and LNCaP cell lines were treated with various concentrations of pectaSol, Dox, or a combination of both.Cell viability was determined via the MTT assay and cell cycle progression was determined by flow cytometry using Flowmax Software.

 

Results: DU-145 (androgen independent) cell lines were more sensitive than LNCaP (androgen dependent) cell lines.Combination of PectaSol and Dox resulted in a 17.6% and 19.6% (p <0.001) decrease in the viability of the DU-145 and LNCaP cells. The IC50 values decreased 1.5 fold and 1.3 fold in the DU-145 and LNCaP cells, respectively. Treatment with both drugs led to an increase in Sub-G1 arrest (54.6% versus 12.2% in Dox) in DU-145. In LNCaP cells, combination of both drugs led to an increase in G2-M arrest (61.68% versus 53.56% in Dox).

 

Conclusion: Based on our findings, the progressive cytotoxicity effect of Dox and PectaSol together induce rapid cell death in DU-145 through apoptosis and in LNCaP cells through cell cycle arrest (G2-M arrest).

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