Volume 18, Issue 92 (5-2011)                   Daneshvar Medicine 2011, 18(92): 69-76 | Back to browse issues page

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Habibi Z, Hassan Z M, Noori S, Mozafari V, Yoosefi-Mohammadi M M, Hassani L. Interaction of salvigenin with DNA. Daneshvar Medicine. 2011; 18 (92) :69-76
URL: http://daneshvarmed.shahed.ac.ir/article-1-278-en.html
Department of Chemistry Shahid Beheshti University
Abstract:   (6861 Views)

Background and Objective: To evaluate interaction of salvigenin with DNA and the molecular mechanism of its anti-cancer effect.

 

Materials and Methods: Salvigenin from Tanacetum canescens and DNA from calf thymus were isolated and purified. Then, salvigenin interaction with DNA in Tris buffer (0.05 M, pH equal to 4.7 and the temperature at 25 °C) was studied by different methods.

 

Results and Conclusion: DNA absorption spectra in the presence of various concentrations of each of these ligands showed that the DNA with salvigenin has interaction and complex formation and the absorption wave length at 260 nm increased. Under the effect of changes in DNA uptake ligand showed structural changes. Also, using information obtained from the suppression with ethidium bromide of DNA complex by ligand skachard, above analysis indicated that the mechanism of suppression of publication is through non-competitive inhibition and it means that connecting mechanism of salvigenin is non-intercalated with DNA and probably through connection small grooves.

Keywords: Salvigenin, DNA, Interaction
Full-Text [PDF 219 kb]   (1118 Downloads)    
Type of Study: Research |

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