Volume 25, Issue 136 (9-2018)                   Daneshvar Medicine 2018, 25(136): 43-54 | Back to browse issues page

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Aminizadeh M, Kavoosi G. The effect of zataria essential oil on superoxide and nitric oxide production and NADH oxidase and nitric oxide synthase in macrophage cells. Daneshvar Medicine. 2018; 25 (136) :43-54
URL: http://daneshvarmed.shahed.ac.ir/article-1-1971-en.html
Institute of Biotechnology, Shiraz University, Shiraz, Iran
Abstract:   (202 Views)
Objective: In this study, inhibitory effect of Zataria multiflora essential oil on superoxide and nitric oxide production and NADH oxidase (NOX) and nitric oxide synthase (NOS) expression was examined.


Materials and Methods: Zataria essential oil was obtained by hydro-distillation and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). Superoxide and nitric oxide scavenging effects of zataria essential oil were tested by biochemical assays. Then, LPS-stimulated macrophages were treated by different concentrations of essential oil and expression of superoxide-producing enzyme and nitric oxide-producing enzyme was investigated by Real-time PCR, also superoxide anion and nitric oxide levels was examined by biochemical assays.


Results: The main components of the zataria essential oil were linalool (32.9%), carvacrol (26.7%), thymol (12.5%), p-Cymene (4.5%) and α-Pinene (2%). Zataria essential oil sequestered superoxide anion (O2-) and nitric oxide (NO) in in vitro assay at low concentrations of 70-100 µg/ml. Zataria essential oil at non-cytotoxic level strongly reduced intracellular reactive oxygen species (ROS) and nitric oxide (NO) production in LPS-stimulated macrophages. Superoxide-producing enzyme and NO producing enzyme expression in LPS-stimulated murine macrophage (cell line J774) was declined by zataria essential oil. 


Conclusion: Due to the results, the in vivo antioxidant activity of zataria essential oil may be attributed to down-production of NO and superoxide and also down-regulation of NOS and NOX expression.
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