Volume 25, Issue 133 (3-2018)                   Daneshvar Medicine 2018, 25(133): 57-66 | Back to browse issues page

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Mohammadyari S, Choobineh S, Soori R, Gaeini A, Hadi H. The effect of aerobic exercise on protein expression of VEGF, Spred 1, and Raf1 in cardiac tissue of rats. Daneshvar Medicine. 2018; 25 (133) :57-66
URL: http://daneshvarmed.shahed.ac.ir/article-1-1880-en.html
Department of Exercise Physiology, Faculty of Physical Education and Sports Science, University of Tehran, Tehran. Iran
Abstract:   (663 Views)
Background and Objective: Diabetes is a metabolic disorder that is associated with dysfunction and impairment of vascular system. Exercise training in diabetic patients improves angiogenesis, which helps treatment of diabetes. The purpose of this study was investigation of aerobic exercise effect on cardiac expression of the proteins Spred-1, Raf1, and VEGF in diabetic rats.
 
Materials and Methods: In this study, 50 rats were randomly divided into 4 groups of diabetic, intact aerobic exercise (8 weeks for 6 sessions per week), diabetic, and intact control. Cardiac muscle was removed and placed immediately in liquid nitrogen. Protein expression of VEGF, Raf-1 and Spred-1 was investigated by ELISA method. Data were analysed using SPSS (version 16.0) at a significance level of p < 0.05. One way ANOVA was used for data analysis with Tukey’s test to find differences between groups.
 
Result: Results showed that diabetes leads to decreased cardiac expression of Raf-1 (p<0.05) and VEGF (p<0.05) proteins and increased cardiac expression of Spred-1 protein (p<0.05). As well, eight weeks of aerobic training led to increase of cardiac expression of Raf-1 (p<0.05) and VEGF (p<0.05) protein and decreased cardiac expression of Spred-1(p<0.05) protein in diabetic rats.
 
Conclusion: Aerobic exercise training increases expression of VEGF and Raf1 protein and decreases expression of Spred-1 in cardiomyocytes, therefore, the effect of diabetes on these proteins in the presence of aerobic exercise is attenuated.
 
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Type of Study: Research | Subject: فیزیولوژی ورزش

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