Volume 25, Issue 132 (1-2018)                   DMed 2018, 25(132): 7-14 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Molaei P, Nasehi M, Hashemi M. The effect of cholestasis and neuroAID treatment on Bcl-xl gene expression in hippocampus of male rats. DMed. 2018; 25 (132) :7-14
URL: http://daneshvarmed.shahed.ac.ir/article-1-1842-en.html
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Abstract:   (6211 Views)
Background and Objective: Cholestasis is bile duct obstruction with accumulation of toxic bile acids in liver and circulatory systems and increased toxicity in hepatocytes. Some studies have shown that cholestasis induces spatial memory deficit. Hippocampus plays an important role in this process. In this research, the effect of cholestasis on expression of BCL-XL (B-cell lymphoma-extra large as genes in apoptosis further) in hippocampus of male rat was assessed. Moreover, the preventive effect of neuroAID (MLC901) on above-mentioned changes regarding gene expression was also evaluated.
Materials and Methods: This project was done on 16 rats weighing 220-240 g, which were divided into three main groups including control, sham and BDL (Bile Duct Ligation). Thereafter, hippocampus region was extracted and the expression level of genes were assessment by Real-Time PCR. Results were analyzed using one-way ANOVA followed by a Tukey test. Significant level was 0.05.
Results: The results of sham-NeuroAID & BDL-NeuroAID groups indicate that NeuroAID significantly increases the expression level of Bcl-Xl gene in comparison with control group. In addition, there was no significant change for subsequent effect of cholestasis in the expression level of Bcl-Xl gene in BDL group.
Conclusion: Results of the current study indicates that NeuroAID can alter negative effect of cholestasis by decreasing apoptosis phenomenon.
Full-Text [PDF 810 kb]   (3251 Downloads)    
Type of Study: Research | Subject: روانشناسی

Add your comments about this article : Your username or Email:

© 2020 All Rights Reserved | Daneshvar

Designed & Developed by : Yektaweb