بررسی اثر پیوند هیدروژل کیتوسان حاوی سلول‌های بنیادی مزانشیمی بر ترمیم زخم سوختگی موش‌های صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد همدان، همدان، ایران

2 مرکز تحقیقات پوست و سلول‌های بنیادی، دانشگاه علوم پزشکی تهران، تهران، ایران

چکیده

مقدمه و هدف: روش سلول درمانی در ترمیم زخم های سوختگی از چالش برانگیزترین     روش های درمانی در دنیا محسوب می‌شود. هدف از این تحقیق تعیین اثر هیدروژل کیتوسان حاوی سلول های بنیادی مزانشیمی مشتق از بافت چربی بر ترمیم زخم سوختگی در مدل حیوانی بود.
مواد و روش ها: این پژوهش به‌صورت تجربی با استفاده از 24 سر موش صحرایی نژاد ویستار انجام شد. زخم سوختگی در پوست موش‌ها ایجاد و موش‌ها به دو گروه کنترل (تحت تیمار با نرمال‌سالین) و گروه تیمار با هیدروژل کیتوسان حاوی سلول‌های بنیادی مزانشیمی مشتق ار بافت چربی تقسیم شدند. روند بهبود زخم سوختگی به‌صورت کمّی و کیفی در روزهای هفتم، چهاردهم و بیست و یکم نسبت به روز صفر مورد بررسی و ارزیابی قرار گرفت. داده ها با استفاده از  روش آماری آنالیز واریانس یک‌طرفه‌ مورد تجزیه و تحلیل قرار گرفتند.
نتایج: غلظت 10 درصد کیتوسان اثر سیتوتوکسیک معناداری بر سلولهای بنیادی مزانشیمی نداشت و دارای قوام و شفافیت مناسب بوده و بررسی با میکروسکوپ SEM نشانگر تخلل مناسب در کیتوسان تهیه شده بود. بررسی بیان مارکرهای تخصصی در سلولهای بنیادی مزانشیمی و پتانسیل تمایز آنها به سلول‌های چربی و استخوان موید ماهیت مزانشیمی سلولها بود. مساحت زخم در روزهای 7، 14 و 21 در گروه تیمار شده با کیتوسان حاوی سلولهای مزانشیمی نسبت به گروه کنترل به طور معناداری کمتر بود.
نتیجه‌گیری: سلول‌های بنیادی مزانشیمی انتقال یافته توسط کیتوسان به محل زخم، باعث ترمیم سریع تر زخم سوختگی شده و بر این اساس، ارزیابی استفاده از این پانسمان در ترمیم زخم های سوختگی در بیماران حایز اهمیت است.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of chitosan hydrogel containing the adipose derived mesenchymal stem cells on burn wound healing in rats

نویسندگان [English]

  • Mahsa Koukaeyan 1
  • Rahim Ahmadi 1
  • Mohammad Ali Nilforoushzadeh 2
  • Sona Zare 2
1 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Hamedan Branch, Hamedan, Iran
2 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
چکیده [English]

Background and Objective: Cell therapy is one of the most challenging methods in the world in repairing burn wounds. The aim of this study was to determine the effect of chitosan hydrogel containing adipose-derived mesenchymal stem cells on burn wound healing in an animal model.
Materials and Methods: This study was performed experimentally using 24 Wistar rats. Burn wounds were created on the skin of rats and the animals were divided into two groups (control treated with normal saline) and the group treated with Chitosan hydrogel containing adipose tissue-derived mesenchymal stem cells. The healing process of burn wounds was evaluated quantitatively and qualitatively after treatment on days 7, 14 and 21 compared to the day 0. The data were analyzed using one-way analysis of variance.
Results: Concentration of 10% chitosan had no significant cytotoxic effect on mesenchymal stem cells and had proper consistency and clarity and SEM microscopy showed suitable porosity in chitosan. The expression of specific markers in mesenchymal stem cells and their potential for differentiation into adipocytes and bone cells confirmed the mesenchymal nature of the cells. Wound area on days 7, 14 and 21 in the chitosan-treated group containing mesenchymal cells was significantly smaller than the control group.
Conclusion: Mesenchymal stem cells transferred by chitosan to the wound site cause accelerated healing of burn wound and therefore, it is important to evaluate the use of this dressing in the healing of burn wounds in patients.

کلیدواژه‌ها [English]

  • Chitosan hydrogel
  • Adipose-derived mesenchymal stem cells
  • Burn wound

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