بررسی اثر فعالیت های ورزشی بر مکانیسم های سلولی و مولکولی (تنظیم کلسیم و رشد سلولی) بافت قلب در موش بزرگ آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران

چکیده

هدف: این مطالعه با هدف بررسی اثر فعالیت های ورزشی بر مکانیسم های سلولی و مولکولی (تنظیم کلسیم و رشد سلولی) بافت قلب صورت گرفت.
مواد و روش ها: روش تحقیق تجربی بوده و به همین منظور تعداد 24 سر موش بزرگ آزمایشگاهی نژاد ویستار با محدوده وزنی20±200 گرم و سن هشت هفته، تهیه و به‌ صورت تصادفی به سه گروه کنترل، تمرین تناوبی شدید و تمرین تداومی تقسیم شدند. پروتکل تمرینی تناوبی شدید؛ 30 دقیقه دویدن تناوبی و گروه تداومی زیر بیشینه (30 تا60 دقیقه) پنج روز در هفته و به مدت 8 هفته اجرا شد. بیان ژن متغیرهای مورد نظر در بافت قلب اندازه گیری شد.
نتایج: بین هشت هفته اجرای تمرین تناوبی شدید و تداومی زیر بیشینه به نسبت گروه کنترل در میزان تغییرات بیان ژن در هر سه متغیر (TGF-ß1،SERCA2a، PI3K) تفاوت معناداری وجود دارد (p<0.05). تمرینات ورزشی تناوبی و تداومی (هر دو نسبت به کنترل) منجر به افزایش معنادار بیان ژن TGF-ß1 در قلب موش های بزرگ آزمایشگاهی نژاد ویستار می‌شود . نتایج نشان داد میزان آنزیم PI3K در تمرین تداومی زیربیشینه و تناوبی شدید افزایش معناداری نسبت به گروه کنترل دارد، اختلافی بین گروه تناوبی شدید و کنترل وجود ندارد و تفاوت معناداری بین دو گروه تمرینی در میزان SERCA2a مشاهده نشد.
نتیجه‌گیری: با توجه به یافته های پژوهش حاضر شدت تمرین بالاتر می تواند تنظیم سلولی و ملکولی را بهتر انجام دهد که نیاز به تحقیقات بیشتری در آینده دارد. لذا یافته های پژوهش حاضر با احتیاط بیان شده است و نیاز به تحقیقات بیشتری در آینده می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of exercise on cellular and molecular mechanisms (regulation of calcium and cell growth) of heart tissue in the rat

نویسندگان [English]

  • Mostafa Setamdideh
  • Kamal Azizbeigi
  • Zaher Eatemad
  • Khalid Mohamadzadeh
Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
چکیده [English]

Objective: The aim of this study was to investigate the effect of exercise on cellular and molecular mechanisms (regulation of calcium and cell growth) of heart tissue.
Materials and Methods: The research method was experimental and for this purpose, 24 male Wistar rats with a weight range of 200±20 g and age of eight-weeks were prepared and randomly divided into three groups: control, intense periodic training and continuous training. Intense periodic training protocol; 30 minutes of intermittent running and sub-maximum continuous group (30 to 60 minutes) were performed five days a week for 8 weeks. Gene expression of the desired variables was measured in cardiac tissue.
Results: There was a significant difference between the eight-weeks of intense and sub-maximum intermittent training compared to the control group in the amount of gene expression changes in all three variables (TGF-ß1, SERCA2a, PI3K)(p<0.05). Intermittent and continuous exercise (both relative to control) leads to a significant increase in  TGF-1 gene expression in the heart of male Wistar rats. The results showed that the amount of PI3K enzyme in the sub-maximal continuous and intense periodic training had a significant increase compared to the control group, there was no difference between the severe periodic group and the control group and there was no significant difference between the two training groups in the amount of SERCA2a.
Conclusion: According to the findings of the present study, higher training intensity can better perform cellular and molecular regulation, which requires more research in the future. The findings of the present study have been cautiously expressed and further research is needed in the future.

کلیدواژه‌ها [English]

  • Endurance training
  • Intense periodic training
  • Calcium deficiency
  • Heart tissue
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