تأثیر تمرین‌ تناوبی با شدت بالا بر محتوای بافت عضلانی پروتئین‌های مایوستاتین و فولیستاتین در موش‌های صحرایی پیر

نوع مقاله : مقاله پژوهشی

نویسندگان

ﮔﺮوه فیزیولوژی ورزﺷﯽ، داﻧﺸﮑﺪه ﻋﻠﻮم ﺗﺮﺑﯿﺘﯽ و رواﻧﺸﻨﺎﺳﯽ، داﻧﺸﮕﺎه ﺷﯿﺮاز، ﺷﯿﺮاز، اﯾﺮان

چکیده

مقدمه و هدف: پروتئین‌های مایوستاتین و فولیستاتین، پروتئین‌های کلیدی در تنظیم بافت عضلانی هستند. سارکوپنیا می‌تواند منجر به اختلال در مکانیسم‌های سلولی و فعالیت این پروتئین‌ها شود. بنابراین هدف از انجام تحقیق حاضر، تأثیر تمرین‌ تناوبی با شدت بالا (HIIT) بر محتوای بافت عضلانی پروتئین‌های مایوستاتین و فولیستاتین در موش‌های صحرایی پیر می‌باشد.
مواد و روش ها: در این مطالعه تجربی، 12 سر موش صحرایی ماده 20 ماهه از نژاد اسپراگوداولی با میانگین وزن 30±250 گرم انتخاب و به روش تصادفی به 2 گروه، تمرین HIIT            (6 سر) و کنترل (6 سر) تقسیم ‏شدند. برنامه تمرینHIIT، 3 روز در هفته و به مدت 8 هفته اجرا شد. برنامه تمرینی با شدت 90-85 درصد VO2max برای تناوب‌های با شدت بالا و شدت 50-45 درصد VO2max برای شدت‌های پایین بود. محتوای پروتئین‌ها از طریق روش آزمایشگاهی وسترن‌بلات اندازه‌گیری شد. برای تجزیه‌وتحلیل داده‌ها از آزمون t مستقل استفاده‏ شد.
نتایج: به دنبال هشت هفته تمرین HIIT، تغییر معنی‌داری در محتوای پروتئین مایوستاتین بین گروه‌های تمرین و کنترل وجود نداشت (49/0=p). در مقابل، محتوای پروتئین فولیستاتین (01/0=p) تفاوت (افزایش) معنی‌داری را نشان داد.
نتیجه‌گیری: با توجه به اینکه هشت هفته تمرین HIIT نتوانست محتوای پروتئین مایوستاتین را تغییر معنی‌داری دهد؛ اما محتوای پروتئین فولیستاتین را افزایش داد که این افزایش می‌تواند منجر به هیپرتروفی عضلانی در موش‌های صحرایی سالمند شود. با این حال، تمرین HIIT به عنوان یک مداخله ورزشی برای سالمندان هنوز ناشناخته است و نیاز به تحقیقات بسیار در زمینه انواع، مدت زمان، شدت‌، تکرار و زمان ریکاوری تمرینات HIIT دارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of high intensity interval training on muscle tissue content of myostatin and follistatin proteins in elderly rats

نویسندگان [English]

  • Seyed Moslem Asadpour
  • Farhad Daryanoosh
  • Mohsen Salesi
  • Javad Nemati
  • Negar kooroshfard
Department of exercise physiology, Faculty of Education and Psychology, Shiraz University, Shiraz, Iran
چکیده [English]

Background and Objective: Myostatin and follistatin proteins are key proteins in the regulation of muscle tissue. Sarcopenia can lead to disruption of cellular mechanisms and the activity of these proteins. Therefore, the aim of the present study was to investigate the effect of high intensity interval training (HIIT) on muscle tissue content of myostatin and follistatin proteins in elderly rats.
Materials and Methods: In this experimental study, twelve female 20-month-old Sprague Dawley rats with an average weight of 250±30 g were selected and randomly divided into 2 groups: HIIT (n=6) and control (n=6). The HIIT program was performed 3 days a week for 8 weeks. The training protocol was 85-90% VO2max for high intensity intervals and 45-50% VO2max for low intensity intervals. Proteins content was measured by Western blotting laboratory method. Independent samples t-test was used to analyze the data.
Results: After eight weeks of HIIT, there was no significant change in myostatin protein content in the training and control groups (P=0.49). In contrast, follistatin protein content (P=0.01) showed a significant difference (Increase).
Conclusion: Considering that eight weeks of HIIT could not significantly change the protein content of myostatin, but it increased the protein content of follistatin which could lead to muscle hypertrophy in elderly rats. However, HIIT as an exercise intervention for the elderly is still unknown and a lot of research on the types, duration, intensity, repetition and recovery time of HIIT is required.

کلیدواژه‌ها [English]

  • High Intensity Interval Training (HIIT)
  • Myostatin protein
  • Follistatin protein
  • Gastrocnemius muscle

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دانشور پزشکی
دوره 29، شماره 4 - شماره پیاپی 155
مهر و آبان 1400
صفحه 66-77

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