بررسی اثر سه شیوه تمرینی هوازی پرشدت، هوازی با شدت متوسط و هوازی تناوبی پرشدت بر بیان ژن‌های PlGF و HGF موش‌های صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران

چکیده

مقدمه و هدف: تمرینات ورزشی اثرات بسیاری بر سلامتی افراد دارند که یکی از این اثرات کمک به آنژیوژنز و جلوگیری از بیماری‌ های قلبی-عروقی است. هدف از مطالعه حاضر بررسی اثر سه شیوه‌ ی تمرینی هوازی پر شدت (HIT)، هوازی با شدت متوسط (MIT) و هوازی تناوبی پر شدت (HIIT) بر روی بیان دو ژن HGF و PlGF که از فاکتورهای مهم دخیل در آنژیوژنز هستند، بود.
مواد و روش ها: در این تحقیق تعداد 32 سر موش صحرایی نر نژاد ویستار با وزن میانگین 300 گرم به طور تصادفی به چهار گروه شامل کنترل، تمرین MIT، HIT و HIIT (8=n) تقسیم شدند و در یک دوره‌ ی هشت هفته‌ ای تحت تمرینات ورزشی قرار گرفتند. 48 ساعت پس از آخرین جلسه‌ ی تمرینی، نمونه‌ برداری از بافت چربی موش‌ ها انجام شد و میزان بیان دو ژن HGF و PlGF در آن‌ ها با استفاده از روش Real time PCR تعیین گردید. تحلیل آماری داده‌ ها با نرم‌افزار SPSS و آزمون‌ های کروسکال والیس و هاجز-هولمز انجام شد.
نتایج: نتایج حاصل نشان داد که هر سه شیوه‌ ی تمرینی MIT، HIT و HIIT سبب افزایش بیان ژن‌ های HGF و PlGF می‌ شوند (P<0.05) با این حال بین سه شیوه‌ ی ورزشی مذکور تفاوت معناداری از این جهت مشاهده نشد (P>0.05).
نتیجه‌گیری: تمرینات ورزشی MIT، HIT و HIIT تغییرات مطلوبی در افزایش بیان ژن‌ های HGF و PlGF دارند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of three methods of training, moderate-intensity aerobic exercise training, high intensity training and high-intensity interval training, on PLGF and HGF gene expression in adipose tissue in rat

نویسندگان [English]

  • Rokhsareh Abedi
  • Saeed Naghibi
  • Majid Gholipour Barzegar
Department of Exercise Physiology, Payame Noor University (PNU), Tehran, Iran
چکیده [English]

Background and Objective: Exercise has many effects on human health, one of which is to help angiogenesis and prevent cardiovascular disease. The aim of this study was to investigate the effect of three training methods Moderate-Intensity Aerobic Exercise Training (MIT), High Intensity Training (HIT) and High-intensity interval training (HIIT) on the expression of HGF and PlGF genes, which are important factors involved in angiogenesis.
Materials and Methods: In this study, 32 male Wistar rats with an average weight of 300 grams were randomly divided into four groups including control, MIT, HIT and HIIT (n=8) and underwent exercise for an eight-week period. 48 hours after the last training session, samples were taken from adipose tissue of rat and the expression of HGF and PlGF genes in them was determined using Real time PCR. Statistical analysis of data was performed using SPSS and Kruskal-Wallis and Hodges-Holmes tests software.
Results: The results showed that all three training methods MIT, HIT and HIIT increase the expression of HGF and PlGF genes (P <0.05). However, there is no significant difference between the three exercise methods (P> 0.05).
Conclusion: MIT, HIT and HIIT exercises have favorable effects in increasing the expression of HGF and PlGF genes.

کلیدواژه‌ها [English]

  • Aerobic exercise
  • Gene expression
  • Placenta Growth Factor
  • Hepatocyte Growth Factor
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