بررسی اثر سه شیوه تمرینی هوازی پرشدت، هوازی با شدت متوسط و هوازی تناوبی پرشدت بر بیان ژن‌های PlGF و HGF موش‌های صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزش، دانشگاه پیام نور، تهران، ایران

چکیده

مقدمه و هدف: تمرینات ورزشی اثرات بسیاری بر سلامتی افراد دارند که یکی از این اثرات کمک به آنژیوژنز و جلوگیری از بیماری‌ های قلبی-عروقی است. هدف از مطالعه حاضر بررسی اثر سه شیوه‌ ی تمرینی هوازی پر شدت (HIT)، هوازی با شدت متوسط (MIT) و هوازی تناوبی پر شدت (HIIT) بر روی بیان دو ژن HGF و PlGF که از فاکتورهای مهم دخیل در آنژیوژنز هستند، بود.
مواد و روش ها: در این تحقیق تعداد 32 سر موش صحرایی نر نژاد ویستار با وزن میانگین 300 گرم به طور تصادفی به چهار گروه شامل کنترل، تمرین MIT، HIT و HIIT (8=n) تقسیم شدند و در یک دوره‌ ی هشت هفته‌ ای تحت تمرینات ورزشی قرار گرفتند. 48 ساعت پس از آخرین جلسه‌ ی تمرینی، نمونه‌ برداری از بافت چربی موش‌ ها انجام شد و میزان بیان دو ژن HGF و PlGF در آن‌ ها با استفاده از روش Real time PCR تعیین گردید. تحلیل آماری داده‌ ها با نرم‌افزار SPSS و آزمون‌ های کروسکال والیس و هاجز-هولمز انجام شد.
نتایج: نتایج حاصل نشان داد که هر سه شیوه‌ ی تمرینی MIT، HIT و HIIT سبب افزایش بیان ژن‌ های HGF و PlGF می‌ شوند (P<0.05) با این حال بین سه شیوه‌ ی ورزشی مذکور تفاوت معناداری از این جهت مشاهده نشد (P>0.05).
نتیجه‌گیری: تمرینات ورزشی MIT، HIT و HIIT تغییرات مطلوبی در افزایش بیان ژن‌ های HGF و PlGF دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of three methods of training, moderate-intensity aerobic exercise training, high intensity training and high-intensity interval training, on PLGF and HGF gene expression in adipose tissue in rat

نویسندگان [English]

  • Rokhsareh Abedi
  • Saeed Naghibi
  • Majid Gholipour Barzegar
Department of Exercise Physiology, Payame Noor University (PNU), Tehran, Iran
چکیده [English]

Background and Objective: Exercise has many effects on human health, one of which is to help angiogenesis and prevent cardiovascular disease. The aim of this study was to investigate the effect of three training methods Moderate-Intensity Aerobic Exercise Training (MIT), High Intensity Training (HIT) and High-intensity interval training (HIIT) on the expression of HGF and PlGF genes, which are important factors involved in angiogenesis.
Materials and Methods: In this study, 32 male Wistar rats with an average weight of 300 grams were randomly divided into four groups including control, MIT, HIT and HIIT (n=8) and underwent exercise for an eight-week period. 48 hours after the last training session, samples were taken from adipose tissue of rat and the expression of HGF and PlGF genes in them was determined using Real time PCR. Statistical analysis of data was performed using SPSS and Kruskal-Wallis and Hodges-Holmes tests software.
Results: The results showed that all three training methods MIT, HIT and HIIT increase the expression of HGF and PlGF genes (P <0.05). However, there is no significant difference between the three exercise methods (P> 0.05).
Conclusion: MIT, HIT and HIIT exercises have favorable effects in increasing the expression of HGF and PlGF genes.

کلیدواژه‌ها [English]

  • Aerobic exercise
  • Gene expression
  • Placenta growth factor
  • Hepatocyte growth factor

مراجع

  1. Zarei M, Khazaei M, Sharifi MR, Pourshanazari AA. Coronary angiogenesis during experimental hypertension: is it reversible? Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences 2011;16(3):269.
  2. Khazaei M, Fallahzadeh AR, Sharifi MR, Afsharmoghaddam N, Javanmard SH, Salehi E. Effects of diabetes on myocardial capillary density and serum angiogenesis biomarkers in male rats. Clinics 2011;66(8):1419-24.
  3. Amjadi F, Javanmard SH, Zarkesh-Esfahani H, Khazaei M, Narimani M. Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production. Journal of Experimental & Clinical Cancer Research 2011;30(1):21.
  4. Auerbach R, Lewis R, Shinners B, Kubai L, Akhtar N. Angiogenesis assays: a critical overview. Clinical Chemistry 2003;49(1):32-40.
  5. Carmeliet P. Mechanisms of angiogenesis and arteriogenesis. Nature Medicine 2000;6(4):389.
  6. Carmeliet P, Moons L, Luttun A, Vincenti V, Compernolle V, De Mol M, et al. Synergism between vascular endothelial growth factor and placental growth factor contributes to angiogenesis and plasma extravasation in pathological conditions. Nature Medicine 2001;7(5):575.
  7. Voros G, Maquoi E, Demeulemeester D, Clerx N, Collen Ds, Lijnen HR. Modulation of angiogenesis during adipose tissue development in murine models of obesity. Endocrinology 2005;146(10):4545-54.
  8. Christiaens V, Lijnen H. Angiogenesis and development of adipose tissue. Molecular and Cellular Endocrinology 2010;318(1-2):2-9.
  9. Saiki A, Watanabe F, Murano T, Miyashita Y, Shirai K. Hepatocyte growth factor secreted by cultured adipocytes promotes tube formation of vascular endothelial cells in vitro. International Journal of Obesity 2006;30(11):1676.
  10. Hausman G, Richardson R. Adipose tissue angiogenesis. Journal of Animal Science 2004;82(3):925-34.
  11. Jansson PA. Endothelial dysfunction in insulin resistance and type 2 diabetes. Journal of Internal Medicine 2007;262(2):173-83.
  12. Bakker W, Eringa EC, Sipkema P, van Hinsbergh VW. Endothelial dysfunction and diabetes: roles of hyperglycemia, impaired insulin signaling and obesity. Cell and Tissue Research 2009;335(1):165.
  13. Goldberg RB. Cytokine and cytokine-like inflammation markers, endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications. The Journal of Clinical Endocrinology & Metabolism 2009;94(9):3171-82.
  14. Cao Y. Adipose tissue angiogenesis as a therapeutic target for obesity and metabolic diseases. Nature Reviews Drug Discovery 2010;9(2):107.
  15. Kolahdouzi S, Talebi-Garakani E, Hamidian G, Safarzade A. Exercise training prevents high-fat diet-induced adipose tissue remodeling by promoting capillary density and macrophage polarization. Life Sciences 2019;220:32-43.
  16. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation 2007;14(6):753-60.
  17. Deschenes M, Maresh C, Crivello J, Armstrong L, Kraemer W, Covault J. The effects of exercise training of different intensities on neuromuscular junction morphology. Journal of Neurocytology 1993;22(8):603-15.
  18. Rognmo Ø, Hetland E, Helgerud J, Hoff J, Slørdahl SA. High intensity aerobic interval exercise is superior to moderate intensity exercise for increasing aerobic capacity in patients with coronary artery disease. European Journal of Cardiovascular Prevention & Rehabilitation 2004;11(3):216-22.
  19. Cao Y. Angiogenesis modulates adipogenesis and obesity. The Journal of Clinical Investigation 2007;117(9):2362-8.
  20. Asgharzade Oliaei HA, Mirdar Harijani S, Mousavi N. The Effects of a Submaximal Endurance Swimming Training Period and Cadmium Poisoning during Pregnancy on the Hepatocyte Growth Factor Response in Neonatal Rats. Qom University of Medical Sciences Journal 2015;9(1):38-45.
  21. harigani s, oliaei h. Effect of swimming endurance training program and Silymarin supplementation during pregnancy on Hepatocyte growth factor levels in neonatal liver tissue. Journal of Applied Sports Physiology 2016;12(23):173-80.
  22. Mosavian A, Gaeini AA, Hemmatinafar M, Kordi MR, Nori R. Effect of low-intensity eccentric resistance training with blood flow restriction on the activation and proliferation indicators of satellite cells. Hormozgan Medical Journal 2018;22(2):95-102.
  23. O'reilly C, McKay B, Phillips S, Tarnopolsky M, Parise G. Hepatocyte growth factor (HGF) and the satellite cell response following muscle lengthening contractions in humans. Muscle & Nerve: Official Journal of the American Association of Electrodiagnostic Medicine 2008;38(5):1434-42.
  24. Gigante B, Tarsitano M, Cimini V, De Falco S, Persico MG. Placenta growth factor is not required for exercise-induced angiogenesis. Angiogenesis 2004;7(3):277-84.
  25. Landers-Ramos RQ, Jenkins NT, Spangenburg EE, Hagberg JM, Prior SJ. Circulating angiogenic and inflammatory cytokine responses to acute aerobic exercise in trained and sedentary young men. European Journal of Applied Physiology 2014;114(7):1377-84.
  26. Vannotti A, Magiday M. Untersuchungen zum Studium des Trainiertseins. Arbeitsphysiologie 1934;7(6):615-22.
  27. Pirouz M, Nourshahi M. The effect of eight weeks training in hypoxia-normobaric and normal situation on serum VEGF, erythropoietin concentration, fatigue index and VO2max 2013.
  28. Egginton S. Invited review: activity-induced angiogenesis. Pflügers Archiv-European Journal of Physiology 2009;457(5):963.
  29. Heil M, Eitenmüller I, Schmitz‐Rixen T, Schaper W. Arteriogenesis versus angiogenesis: similarities and differences. Journal of Cellular and Molecular Medicine 2006;10(1):45-55.
  30. Laughlin M, Roseguini B. Mechanisms for exercise training-induced increases in skeletal muscle blood flow capacity: differences with interval sprint training versus aerobic endurance training. Journal of Physiology and Pharmacology: an Official Journal of the Polish Physiological Society 2008;59 (Suppl 7):71.
  31. Nourshahi M, Ranjbar K. The stimulus of angiogenesis during exercise and physical activity. Quarterly of the Horizon of Medical Sciences 2013:286-96.
دانشور پزشکی
دوره 29، شماره 4 - شماره پیاپی 155
مهر و آبان 1400
صفحه 55-65

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