اثر تمرین تناوبی شدید بر بیان ژن‌های HSP-72 و TGF-β بافت کلیه و برخی نشانگرهای سرمی کلیه در موش‌های صحرایی دیابتی نوع-II

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

مقدمه و هدف: بیماری کلیه دیابتی (DKD) یکی از اختلالات بسیار مهم دیابت است. پروتئین شوک گرمایی 72 (HSP72) و فاکتور رشد تغییردهنده بتا (TGFβ) به­عنوان نشانگرهای راهبردی در حفاظت کلیه مورد توجه می­باشند. تمرین تناوبی شدید (HIIT) نیز اثرات مثبتی بر مدیریت دیابت دارد. هدف از مطالعه حاضر، بررسی اثر HIIT بر بیان ژن­های HSP72 و TGFβ در بافت کلیه موش­های صحرایی دیابتی نوع 2 بود.
مواد و روش ها: بیست و یک سر موش صحرایی به­طور تصادفی در سه گروه (7=تعداد) تقسیم شدند: کنترل، دیابت و دیابت+HIIT. القای دیابت نوع 2 با تزریق درون صفاقی نیکوتین آمید و استرپتوزتوسین انجام شد. گروه دیابت+HIIT چهار هفته برنامه ورزشی داشتند. بیست و چهار ساعت پس از آخرین جلسه تمرین، همه حیوانات تشریح شدند. نمونه­های خونی برای سنجش سطح گلوکز، انسولین، کراتینین، اوره و اسید اوریک پلاسما جمع شد. کلیه چپ جدا و هموژنایز شد و بیان ژن­های مذکور با استفاده از Real-time PCR صورت گرفت.
نتایج: : دیابت به­طور معنی­داری بیان ژن TGFβ را افزایش و بیان HSP72 را کاهش داد. در مقایسه با گروه دیابت، HIIT سطح گلوکز پلاسما را اندکی کاهش داد که معنی­دار نبود؛ با این حال، HIIT شاخص مقاومت به انسولین را بهبود داد (03/0=p). تغییرات کراتینین معنی­دار نبود اما اسید اوریک در گروه دیابت+HIIT کاهش معنی­داری داشت (02/0=p). همچنین، HIIT بیان ژن TGFβ را کاهش و HSP72 را افزایش داد (001/0=p).
نتیجه‌گیری: یافته­های ما نشان داد HIIT به­عنوان روش مداخله غیردارویی، احتمالا اثرات محافظتی بر کلیه موش­های صحرایی دیابتی نوع 2 دارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effects of high-intensity interval training on TGF-β and HSP72 gene expression in kidney tissue and selective renal serum biomarkers of type-II diabetic rats

نویسندگان [English]

  • Mostafa Baranchi
  • Hamid Agha-Alinejad
Department of Exercise Physiology, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Background and Objective: Diabetic Kidney Disease (DKD) is one of the most significant complication of diabetes. Heat Shock Protein 72 (HSP72) and Transforming Growth Factor beta (TGF-β) are regarded as biomarkers for renoprotective strategies. High-intensity interval training (HIIT) may have beneficial effect on diabetes management. The purpose of the present study was to investigate the effects of HIIT on kidney expression of HSP72 and TGF-β in type-II diabetic (T2D) rats.
Materials and Methods: 21 rats were randomly divided into three groups as follows: control (Con), diabetic (Dibt), diabetic HIIT (Dibt-HIIT). Nicotinamide and streptozotocin were used intraperitoneally for diabetes induction. The Dibt-HIIT received four-week HIIT training. 24 hours following the last training session, all animals were anesthetized. Blood samples were collected for measuring the glucose, insulin, creatinine, urea and uric acid concentrations. The left kidney was removed and homogenized for measuring of HSP72 and TGF-βgene expression (through Real-Time PCR).
Results: Diabetes significantly increased gene expression of TGF-β and suppressed HSP-72 gene expression. Compared to Dibt group, HIIT decreased plasma glucose level but these changes were not significant. However, HIIT improved HOMA-IR (p=0.03). Creatinine change was not significant but Uric acid decreased in Dibt-HIIT group significantly (p=0.02).  Also, HIIT reduced kidney expression of TGF-β and increased HSP72 expression in Dibt-HIIT group (p=0.001).
Conclusion: Our findings indicated that HIIT, as a non-pharmacologic approach, may have protective effect on kidney of rats with T2D.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • Type-II diabetes
  • Kidney
  • HSP72
  • TGFβ

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دانشور پزشکی
دوره 29، شماره 4 - شماره پیاپی 155
مهر و آبان 1400
صفحه 42-54

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