بررسی اثر عصاره هیدرو الکلی برگ گیاه شاهتره بر میزان درد و تشنج ناشی از تزریق پنتلین تترازول در موشهای صحرایی کوچک نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

3 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: مطالعات زیادی در مورد مکانیسم های درمان تشنج و کاهش درد وجود دارد. به هرحال با توجه به توصیه های طب گیاهی در این مطالعه ازعصاره برگ گیاه شاهتره برای تسکین درد و تشنج در موش های کوچک صحرایی استفاده شده است.
مواد و روش ها: در این  بررسی 40 سر موش صحرایی کوچک به 5 گروه 1-کنترل 2-کنترل مثبت و سه گروه عصاره  با دوزهای 200، 600 و 800 میلی گرم برکیلوگرم تقسیم شدند. همه حیوانات وارد آزمون فرمالین و قوطه وری دم در آب برای آنالیز درد شدند سپس با تزریق پنتیلن تترازول (mg/kg 100) و محاسبه زمان شروع علائم میوکلونوس، کلونوس و تونوس به بررسی تشنج در این موش ها پرداخته شد. 
یافته‌ها: نتایج نشان داد عصاره در سه دوز  200، 600 و 800 میلی گرم بر کیلوگرم می تواند باعث کاهش درد حاد و مزمن ناشی از فرمالین شود. همچنین زمان شروع رفتارهای تشنج یعنی میوکلونوس، کلونوس و تشنج می تواند با دوزهای کمتر عصاره 200 و 600 میلی گرم بر کیلوگرم به طور قابل توجهی افزایش یابد.
نتیجه گیری: بطور کلی درمان موش ها باعصاره هیدروالکلی برگ گیاه شاهتره می تواند باعث کاهش درد حاد و مزمن گشته و زمان شروع علائم تشنج را بطور معنی داری افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of hydroalcoholic extract of fumaria officinalis leaf on pain and seizure induced by pentylentetrazole in male mice

نویسندگان [English]

  • Fatemeh Taleahmad 1
  • Mehdi Alizaedeh 2
  • Fatemeh Nabi 2
  • Zahra Kiasalari 3
  • Mohsen Khalili 3
  • Fariba Ansari 2
1 Student Research Center, School Of Medicine, Shahed University, Tehran, Iran
2 School of Medicine, Shahed University, Tehran, Iran
3 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: There are many reports for mutual mechanisms for seizure and pain alleviation. Also with respect to the herbal medicine recommendation in new medicine, in this study we used the extract of an important candidate fumaria officinal for relief of pain and seizure in mice.
Materials and Methods: In our experiments 40 mice were divided to 5 groups (n=8): 1-control 2- positive control and three groups of extract treatment respectively 200, 600 and 800 mg/kg. All of the animal groups were conducted to formalin and tail immersion tests for assessment of pain and finally seizure analysis were done by injection of PTZ (100 mg/kg) to the animals and initiation time (s) for myoclonus, clonus and the tonus signs were measured for seizure analysis.
Results: Our results indicated that the extract in three doses (200, 600 and 800 mg/kg) can reduced the formalin acute and chronic pain. Also the start time for seizure signs i.e, myoclonus, clonus and seizures could elevated markedly in lower doses of the extract (200 and 600 mg/kg).
Conclusion: In addition, treatment of the mice with hydroalcoholic extract of fumaria officinalis leaf could significantly reduce the acute and chronic pain and increase the initiation time of the seizure signs.

کلیدواژه‌ها [English]

  • Pain
  • Formalin
  • Seizure
  • PTZ
  • Fumaria officinalis leaf
  1. Maldacker J. Preclinical investigations with mistletoe (Viscum album L.) extract Iscador. Arzneimittel for schung 2006; 56(6A):497-507. doi: 10.1055/s-0031-1296817.
  2. Bjorkman R. Central antinociceptic effect of non-steroidal anti-Inflammatory drugs and paracetamol. Acta Anaesthesiol Scand 1995; 103:1-44.
  3. Woolf CG. Maninion RJ. Neuropathic pain: aetiology, symptoms, mechanisms, and management. Lancet. 1999; 353(9168):1959-64. doi: 10.1016/S0140-6736(99)01307-0.
  4. Reivik B, Borchgrevink H, Allen PC, Rose Land SM, Hals L, Kvaristain EK, et al. Assessment of pain. British Journal of Anaesthasia. 2008; 101(1):17-24. doi: 10.1093/bja/aen103.
  5. Gebhart GF, Bielefeldt K. Physiology of Visceral Pain. Comprehensive Physiology 2016; 6(4):1609-1633. doi: 10.1002/cphy.c150049.
  6. Jiang H, Cui H, Wang T, Shimada S, Sun R, Tan Z, Ma c, La Motte RH. CCL2/CCR2 signaling elicits itch- and pain-like behavior in a murine model of allergic contact dermatitis. Brain, Behavior, and Immunity 2019; 464-473. doi: 10.1016/j.bbi.2019.04.026.
  7. Metcalf CS, Huntsman M, Garcia G, Kochanski AK, Chikinda M, Watanabe E, et al. Music-Enhanced Analgesia and Antiseizure Activities in Animal Models of Pain and Epilepsy: Toward Preclinical Studies Supporting Development of Digital Therapeutics and Their Combinations With Pharmaceutical DrugsFrontiers in Neurology 2019; 10:277. doi: 10.3389/fneur.2019.00277. eCollection 2019.
  8. Huber R, Ludtke R. Effects of a mistletoe preparation with defined lectin content on chronic hepatitis C: an individually controlled cohort study. European Journal Medicine Research 2001; 6(9):399-405. PMID: 11669085
  9. Onay-Ucar E, Karagon A, Arda N . Antioxidant activity of Viscum album ssp. album. Fitoterapia 2006; 556-60.
  10. Katzung B.G. Basic and clinical pharmacology. ISBN: 978-0-07-176402-5 MHID: 0-07-176402-X The material in this eBook also appears in the print version of this title: ISBN: 978-0-07-176401-8, MHID: 0-07-176401-1.
  11. Long X, Yuan F, Zhao J, Song C, Zhao Z, Zhang Y, et al. Long-term seizure outcome in patients with status epilepticus due to acute encephalitis. Seizure 2019; 69:70-75. doi: 10.1016/j.seizure.2019.04.005.
  12. Huang R, Bell-horner CL, Dibas MI, Covey DF, Drewe J A, Dillon G H. Pentylenetetrazole-induced inhibition of recombinant gamma-aminobutyric acid type A (GABA(A)) receptors: mechanism and site of action. Journal of Pharmacology and Experimental Therapeutics 2001; 298(3):986-95. PMID: 11504794
  13. Davis R, Peters. D.H and Mc Taris D.B. Valproic acid a reappraisal of its pharmacological properties and clinical efficacy in epilepsy. Drugs 1994; 47(2):332-72.
  14. Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson DL, et al. Harrison’s Principles of Internal Medicine 2012; 18.
  15. Sepehri G.R, Sheibani V, Pahlavan Y, Afarinesh Khaki MR, Esmail Pour Bezenjani Kh, Pahlavan B. Effect of intracerebroventricular injection of aqueous extract of Origanum vulgare L. ssp. virde on pain threshold in male rats. Journal of Ardabil University of Medical Sciences 2011; 11(1): 52-58
  16. Ghasemi Dehkordi NA, Sajadi SE, Ghanadi AR, Amanzadeh Y, Azadbakht M, Asghari GHR, et al. Iranian Herbal Pharmacopoeia.  Hakim research Journal 2003; 6(3): 63-69.
  17. Mozafarian,V. Dictionary of Iranian Plant Names: Latin-English-Persian. ASIN: B005SFUIGI. Publisher: Farhang Moaser (January 1, 1998). ISBN-10: 9645545404. ISBN-13: 978-9645545404. P: 238.
  18. Zargari A. Medicinal plants. Seventh chap. Tehran University Publishing, Tehran 1376; 72: 166.
  19. Jowkar F, Jamshidzadeh A, Mirzadeh Yazdi A, Pasalar M. The effects of fumaria parviflora L extract on chronic hand eczema: a randomized double-blind placebo controlled clinical trial. Iranian Red Crescent Medical Journal 2011; 13(11):824-8. PMID: 22737422.
  20. Gilani Ah, Janbaz Kh, Akhtar Ms. Selective protective effect extract from Fumaria parviflora on paracetamol-induced hepatotoxicity. General Pharmacology 1996; 27(6):979-83. doi: 10.1016/0306-3623(95)02140-x.
  21. Bribi N, Algieri F, Rodriguez-Nogales A, Vezza T, Garrido-Mesa J, Utrilla MP, et al. Intestinal anti-inflammatory effects of total alkaloid extract from Fumaria capreolata in the DNBS model of mice colitis and intestinal epithelial CMT93 cells. Phytomedicine 2016; 23(9):901-13. doi: 10.1016/j.phymed.2016.05.003.
  22. Miladi Gorji H, Rashidi Pour A, Vafaei AA, Taherian AA. Opioid receptors role on anti-nociceptive effects of the aqueous extracts of Melissa Officinalis in mice. Hormozgan Medical Journal 2006; 10(1):23-28.
  23. Dashti-Rahmatabadi M, Vahidi Merjardi A, Pilavaran A, Farzan F. Antinociceptive effect of cinnamon extract on formalin-induced pain in Rat. Journal of Shahid Sadoughi University of Medical Sciences 2009; 17 (2):190-199.
  24. Gelgor L, Butkow N, Mitchell D. Effects of systemic non-steroidal anti-inflammatory drugs on nociception during tail ischaemia and on reperfusion hyperalgesia in rats. British Journal of Pharmacology 1992; 105(2): 412-416. https://doi.org/10.1111/j.1476-5381.1992.tb14267.x
  25. KanuiTI, Karim F, Towett PK. The formalin test in the naked male rats (Heterocephalus glaber): analgesic effects of morphine, nefopam and paracetamol. Brain Research 1993; 600(1):123-6. doi: 10.1016/0006-8993(93)90409-g.
  26. Janahmadi M, Niazi F, Danyali S. Effects of the fruit essential oil of Cuminum cyminum Linn. (Apiaceae) on pentylenetetrazol-induced epileptiform activity in F1 neurones of Helix aspersa.  Journal of Ethnopharmacology 2006; 104(1-2):278-82. doi: 10.1016/j.jep.2005.09.019.
  27. Fred H. Cate. Transportation of Animals – Guidelines. Institutional Animal Care and Use Committee (IACUC) Office of Research Compliance (ORC). Bloomington, IN 47408. https://research.iu.edu/policies/bloomington-animal-care-and-use.html.
  28. Dray A, Urban L. New Pharmacological strategies for pain relief. Annual Review of Pharmacology and Toxicology 1996; 36:253-80. doi: 10.1146/annurev.pa.36.040196.001345.
  29. Porter M.R. Handbook of surfactants 1994; 168-200. ISBN 978-0-7514-0170-7. Springer Netherlands.
  30. Tjolsen A, Berge O.G, Hunskaar S, Rosland J.H, Hole K. The formalin test: an evaluation of the method. Pain 1992; 51(1):5-17. doi: 10.1016/0304-3959(92)90003-T.
  31. Rebrov IG, Karpova MN, Andreev AA,Kuzina OS, Kalinina MS, Abrosimove IY, et al. Effect of single injection of pentylenetetrazole in a subconvulsive dose on Cl- conductance of the GABAA-receptor complex. Bulletin of Experimental Biology and Medicine 2004; 137(1):13-6. doi: 10.1023/b:bebm.0000024374.01961.23.
  32. Eisenberger NI, Liebman M. Why it hurts to be left out: The neurocognitive overlap between physical and social pain. American Psychological Association. 2005. Corpus ID: 16944310.