بررسی اثر عصاره هیدرو الکلی برگ گیاه شاهتره بر میزان درد و تشنج ناشی از تزریق پنتلین تترازول در موشهای صحرایی کوچک نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

3 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: مطالعات زیادی در مورد مکانیسم های درمان تشنج و کاهش درد وجود دارد. به هرحال با توجه به توصیه های طب گیاهی در این مطالعه ازعصاره برگ گیاه شاهتره برای تسکین درد و تشنج در موش های کوچک صحرایی استفاده شده است.
مواد و روش ها: در این  بررسی 40 سر موش صحرایی کوچک به 5 گروه 1-کنترل 2-کنترل مثبت و سه گروه عصاره  با دوزهای 200، 600 و 800 میلی گرم برکیلوگرم تقسیم شدند. همه حیوانات وارد آزمون فرمالین و قوطه وری دم در آب برای آنالیز درد شدند سپس با تزریق پنتیلن تترازول (mg/kg 100) و محاسبه زمان شروع علائم میوکلونوس، کلونوس و تونوس به بررسی تشنج در این موش ها پرداخته شد. 
یافته‌ها: نتایج نشان داد عصاره در سه دوز  200، 600 و 800 میلی گرم بر کیلوگرم می تواند باعث کاهش درد حاد و مزمن ناشی از فرمالین شود. همچنین زمان شروع رفتارهای تشنج یعنی میوکلونوس، کلونوس و تشنج می تواند با دوزهای کمتر عصاره 200 و 600 میلی گرم بر کیلوگرم به طور قابل توجهی افزایش یابد.
نتیجه گیری: بطور کلی درمان موش ها باعصاره هیدروالکلی برگ گیاه شاهتره می تواند باعث کاهش درد حاد و مزمن گشته و زمان شروع علائم تشنج را بطور معنی داری افزایش دهد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of hydroalcoholic extract of fumaria officinalis leaf on pain and seizure induced by pentylentetrazole in male mice

نویسندگان [English]

  • Fatemeh Taleahmad 1
  • Mehdi Alizaedeh 2
  • Fatemeh Nabi 2
  • Zahra Kiasalari 3
  • Mohsen Khalili 3
  • Fariba Ansari 2
1 Student Research Center, School Of Medicine, Shahed University, Tehran, Iran
2 School of Medicine, Shahed University, Tehran, Iran
3 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: There are many reports for mutual mechanisms for seizure and pain alleviation. Also with respect to the herbal medicine recommendation in new medicine, in this study we used the extract of an important candidate fumaria officinal for relief of pain and seizure in mice.
Materials and Methods: In our experiments 40 mice were divided to 5 groups (n=8): 1-control 2- positive control and three groups of extract treatment respectively 200, 600 and 800 mg/kg. All of the animal groups were conducted to formalin and tail immersion tests for assessment of pain and finally seizure analysis were done by injection of PTZ (100 mg/kg) to the animals and initiation time (s) for myoclonus, clonus and the tonus signs were measured for seizure analysis.
Results: Our results indicated that the extract in three doses (200, 600 and 800 mg/kg) can reduced the formalin acute and chronic pain. Also the start time for seizure signs i.e, myoclonus, clonus and seizures could elevated markedly in lower doses of the extract (200 and 600 mg/kg).
Conclusion: In addition, treatment of the mice with hydroalcoholic extract of fumaria officinalis leaf could significantly reduce the acute and chronic pain and increase the initiation time of the seizure signs.

کلیدواژه‌ها [English]

  • Pain
  • formalin
  • Seizure
  • PTZ
  • Fumaria officinalis leaf

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دانشور پزشکی
دوره 29، شماره 2 - شماره پیاپی 153
خرداد و تیر 1400
صفحه 31-41

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