اثرات عصاره آبی- الکلی لوندولا آفیسینالیس (اسطوخودوس انگلیسی) در افسردگی القا شده توسط رزرپین در موشهای کوچک آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات نوروفیزیولوژی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: افسردگی یکی از پنج بیماری شابع در جهان با شیوع % 4.4 است و اسطوخودوس برای درمان آن پیشنهاد می شود. هدف از این مطالعه بررسی اثرات عصاره اسطوخودوس انگلیسی در مدل افسردگی ناشی از رزرپین بر روی موش های کوچک آزمایشگاهی بوده است.
مواد و روش ها: دو عدد موش کوچک آزمایشگاهی به طور تصادفی به نه گروه تقسیم بندی شدند: نرمال سالین، کنترل عصاره (200 mg/kg) ، رزرپین، فلوکستین + (10 or 20 mg/kg) رزرپین، فلوکستین (10 mg/kg) + عصاره (200 mg/kg) + رزرپین، سه گروه پیش درمان شده با عصاره + (100-200 and 400 mg/kg) رزرپین. عصاره و فلوکستین توسط گاواژ روزانه به مدت ده روز، سی دقیقه قبل از تزریق داخل صفاقی رزرپین (0.5 mg/kg) تجویز شده است. ارزیابی رفتاری توسط تست های شنای اجباری ، تعلیق دم، ماز به علاوه ای مرتفع و open field انجام شده است .
نتایج: زمان بی حرکتی در تست شنای اجباری توسط رزرپین افزایش یافت (210.37±2.43 در مقایسه با نرمال سالین 109.75±3.13) و عصاره مثل فلوکستین به طور وابسته به دوز این زمان را کاهش داد) به ترتیب  140.75± 5.84و 110.125±6.46 برای دوزهای 200 و 400 (  .p <0.05ترکیب عصاره و فلوکستین موثرتر از هر کدام به تنهایی عمل کرد. نتایج به دست آمده از تست تعلیق دم نیز مشابه تست شنای اجباری بود.
نتیجه‌گیری: عصاره ی آبی- الکلی L. officinalis منجر به بهبود علائم شبه افسردگی ناشی از رزرپین شد. در این مورد اثر ترکیب عصاره و فلوکستین قابل جمع شدن بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Lavandula officinalis hydroalchoholic extract on mouse reserpine induced depression

نویسندگان [English]

  • Banafsheh Bagheri 1
  • Batool Rahmati 2
  • Faezeh Ghozatloo 1
  • Mehrdad Roughani 2
1 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran
2 Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: Depression is one of the five most common diseases in the world with a prevalence of 4.4%, and Ustokhoddus is recommended for its treatment. This study was designed to determine the effects of Lavandula. officinalis extract on a mouse model of reserpine-induced depression.
Materials and Methods: Seventy-two mice were randomly divided into 9 groups: Normal saline, control extract (200 mg/kg), reserpine, fluoxetine (10 mg/kg or 20 mg/kg) + reserpine, fluoxetine (10 mg/kg) + extract (200 mg/kg) + reserpine, Three extract pretreated groups (100-200 and 400 mg/kg) + reserpine. Extract and fluoxetine were administrated by gavages daily, for 10 days, 30 min before reserpine (0.5 mg/kg) injection intraperitoneally. Behavioral evaluations were done by forced swimming, tail suspension, elevated plus maze and open field tests.
Results: Immobility time was enhanced by reserpine (210.37 ± 2.43 as compared with normal saline 109.75 ± 3.13) and the extract decreased it, dose-dependently (140.75± 5.84 and 110.125 ± 6.46, 200 and 400 mg/kg respectively) as the same as fluoxetine (p < 0.05), in forced swimming test. The combination of extract and fluoxetine caused a reduction of immobility time more effective than each one alone. The results obtained from tail suspension were similar to the forced swimming test.
Conclusion: L. officinalis hydroalcoholic extract improved the depression-like behavior caused by reserpine. The effect of the combination of the extract and fluoxetine was additive.

کلیدواژه‌ها [English]

  • Reserpine
  • Lavandula officinalis
  • Ustokhoddus
  • Depression
1.      Rahmati B, Kiasalari Z, Roghani M, Khalili M, Ansari F. Antidepressant and anxiolytic activity of Lavandula officinalis aerial parts hydroalcoholic extract in scopolamine-treated rats. Pharmaceutical Biology 2017;55(1):958-965. doi: 10.1080/13880209.2017.1285320.
2.      Greenwood R, Zhao J, Ludrick B, Golden T, Wu N. A practical animal model for depression by reserpine-administered mice. Theranostics of Brain, Spine & Neural Disorders 2018; 3(2): 26-28.
3.      Garcia-Ceja E, Riegler M, Jakobsen P, Torresen J, Nordgreen T, Oedegaard KJ, et al., editors. Motor activity based classification of depression in unipolar and bipolar patients. 2018 IEEE 31st International Symposium on Computer-Based Medical Systems (CBMS).  doi:10.1109/CBMS.2018.00062. Corpus ID: 50779937
4.      Nikfarjam M, Rakhshan R, Ghaderi H. Comparison of effect of Lavandula officinalis and venlafaxine in treating depression: A double blind clinical trial. Journal of Clinical and Diagnostic Research. 2017;11(7):KC01-KC04. doi: 10.7860/JCDR/2017/20657.10233.
5.      WHO. Other common mental disorders: global health estimates. Geneva: World Health Organization. 2017:1-24.WHO reference number: WHO/MSD/MER/2017.2.
6.      Amoateng P, Kukuia KKE, Mensah JA, Osei-Safo D, Adjei S, Eklemet AA, et al. An extract of Synedrella nodiflora (L) Gaertn exhibits antidepressant properties through monoaminergic mechanisms. Metabolic Brain Disease 2018;33(4):1359-1368. doi: 10.1007/s11011-018-0244-0.
7.      Zuckerman H, Pan Z, Park C, Brietzke E, Musial N, Shariq AS, et al. Recognition and treatment of cognitive dysfunction in major depressive disorder. Frontiers in psychiatry. 2018;9:655. doi: 10.3389/fpsyt.2018.00655.
8.      Perez-Caballero L, Torres-Sanchez S, Bravo L, Mico JA, Berrocoso E. Fluoxetine: a case history of its discovery and preclinical development. Expert opinion on drug discovery 2014;9(5):567-78.doi: 10.1517/17460441.2014.907790.
9.     
34
 
Latendresse G, Elmore C, Deneris A. Selective Serotonin Reuptake Inhibitors as First-Line Antidepressant Therapy for Perinatal Depression. Journal of Midwifery & Women's Health 2017;62(3):317-28.doi: 10.1111/jmwh.12607.
10.  Rheker J, Winkler A, Doering BK, Rief W. Learning to experience side effects after antidepressant intakeResults from a randomized, controlled, double-blind study. Psychopharmacology 2017;234(3):329-38.doi: 10.1007/s00213-016-4466-8.
11.  Ranjbar M, Salehi A, Rezaeizadeh H, Zarshenas MM, Sadeghniiat-Haghighi K, Mirabzadeh M, et al. Efficacy of a Combination of Melissa officinalis L. and Nepeta menthoides Boiss. & Buhse on Insomnia: A Triple-Blind, Randomized Placebo-Controlled Clinical Trial. The Journal of Alternative and Complementary Medicine 2018;24(12):1197-203.doi: 10.1089/acm.2017.0153
12.  Kim N-S, Lee D-S. Comparison of different extraction methods for the analysis of fragrances from Lavandula species by gas chromatographymass spectrometry. Journal of Chromatography A 2002;982(1):31-47.doi: 10.1016/s0021-9673(02)01445-0.
13.  Abbasi Maleki S, Bekhradi R, Asgharpanah J, Abbasi Maleki F, Maleki Ahanghari N. Antidepressant-Like effect of aqueous and hydroalcoholic extracts of Lavandula angustifolia Mill in forced swim test and tail suspension test in male mice. Journal of Arak University of Medical Sciences 2013;16(9):65-75.
14. 
35
 
Chen SL, Chen CH. Effects of Lavender tea on fatigue, depression, and maternalinfant attachment in sleepdisturbed postnatal women. Worldviews on EvidenceBased Nursing 2015;12(6):370-9.doi: 10.1111/wvn.12122.
15.  Mahajan S, Engel WK. Assessment: symptomatic treatment for muscle cramps (an evidence-based review): report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurolog. Neurology 2010;75(15):1397-8. PMID: 20960612.
16.  Nikfarjam M, Rakhshan R, Ghaderi H. Comparison of Effect of Lavandula officinalis and Venlafaxine in Treating Depression: A Double Blind Clinical Trial. Journal of Clinical and Diagnostic Research 2017;11(7):KC01-KC4. doi: 10.7860/JCDR/2017/20657.10233.
17.  Park B-K, Kim YR, Kim YH, Yang C, Seo C-S, Jung IC, et al. Antidepressant-like effects of gyejibokryeong-hwan in a mouse model of reserpine-induced depression. Biomed Research International 2018;5845491. doi: 10.1155/2018/5845491.
18.  Saki K, Bahmani M, Rafieian-Kopaei M. The effect of most important medicinal plants on two importnt psychiatric disorders (anxiety and depression)-a review. Asian Pacific Journal of Tropical Medicine 2014;7:S34-S42.doi: 10.1016/S1995-7645(14)60201-7.
19.  Xian Y-F, Fan D, Ip S-P, Mao Q-Q, Lin Z-X. Antidepressant-like effect of isorhynchophylline in mice. Neurochemical Research 2017;42(2):678-685. doi: 10.1007/s11064-016-2124-5.
20.  Wang Y, Gao S-M, Li R, Zhang M, Gao S, Yu C-Q. Antidepressant-like effects of the Radix Bupleuri and Radix Paeoniae Alba drug pair. Neuroscience Letters 2016;633:14-20.doi: 10.1016/j.neulet.2016.09.001
21.  Seibenhener ML, Wooten MC. Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice. Journal of Visualized Experiments 2015(96):e52434.doi: 10.3791/52434.
22.  Page CE, Shepard R, Heslin K, Coutellier L. Prefrontal parvalbumin cells are sensitive to stress and mediate anxiety-related behaviors in female mice. Scientific Reports 2019;9(1):1-9.doi: 10.1038/s41598-019-56424-9.
23.  Kageyama A, Ueno T, Oshio M, Masuda H, Horiuchi H, Yokogoshi H. Antidepressant-like effects of an aqueous extract of lavender (Lavandula angustifolia Mill.) in rats. Food Science and Technology Research. 2012;18(3):473-9.doi.org/10.3136/fstr.18.473.
24.  Sarris J, Panossian A, Schweitzer I, Stough C, Scholey A. Herbal medicine for depression, anxiety and insomnia: a review of psychopharmacology and clinical evidence. European neuropsychopharmacology. 2011;21(12):841-60.doi: 10.1016/j.euroneuro.2011.04.002.
25. 
36
 
Abuhamdah S, Chazot PL. Lemon Balm and Lavender herbal essential oils: Old and new ways to treat emotional disorders?. Current Anaesthesia & Critical Care. 2008;19(4):221-6.doi.org/10.1016/j.cacc.2008.05.005.
26.  Akhondzadeh S, Kashani L, Fotouhi A, Jarvandi S, Mobaseri M, Moin M, et al. Comparison of Lavandula angustifolia Mill. tincture and imipramine in the treatment of mild to moderate depression: a double-blind, randomized trial. Progress in Neuro-Psychopharmacology and Biological Psychiatry 2003;27(1):123-7. doi: 10.1016/s0278-5846(02)00342-1.
27.  Koulivand PH, Khaleghi Ghadiri M, Gorji A. Lavender and the nervous system. Evidence-Based Complementary and Alternative Medicine. 2013;2013:681304. doi: 10.1155/2013/681304.
28.  Kyzar E, Stewart AM, Landsman S, Collins C, Gebhardt M, Robinson K, et al. Behavioral effects of bidirectional modulators of brain monoamines reserpine and d-amphetamine in zebrafish. Brain research. 2013;1527:108-16.doi: 10.1016/j.brainres.2013.06.033.
Angrini M, Leslie JC, Shephard RA. Effects of propranolol, buspirone, pCPA, reserpine, and chlordiazepoxide on open-field behavior. Pharmacology Biochemistry and Behavior 1998;59(2):387-97.doi: 10.1016/s0091-3057(97)00457-7.
1.      Rahmati B, Kiasalari Z, Roghani M, Khalili M, Ansari F. Antidepressant and anxiolytic activity of Lavandula officinalis aerial parts hydroalcoholic extract in scopolamine-treated rats. Pharmaceutical Biology 2017;55(1):958-965. doi: 10.1080/13880209.2017.1285320.
2.      Greenwood R, Zhao J, Ludrick B, Golden T, Wu N. A practical animal model for depression by reserpine-administered mice. Theranostics of Brain, Spine & Neural Disorders 2018; 3(2): 26-28.
3.      Garcia-Ceja E, Riegler M, Jakobsen P, Torresen J, Nordgreen T, Oedegaard KJ, et al., editors. Motor activity based classification of depression in unipolar and bipolar patients. 2018 IEEE 31st International Symposium on Computer-Based Medical Systems (CBMS).  doi:10.1109/CBMS.2018.00062. Corpus ID: 50779937
4.      Nikfarjam M, Rakhshan R, Ghaderi H. Comparison of effect of Lavandula officinalis and venlafaxine in treating depression: A double blind clinical trial. Journal of Clinical and Diagnostic Research. 2017;11(7):KC01-KC04. doi: 10.7860/JCDR/2017/20657.10233.
5.      WHO. Other common mental disorders: global health estimates. Geneva: World Health Organization. 2017:1-24.WHO reference number: WHO/MSD/MER/2017.2.
6.      Amoateng P, Kukuia KKE, Mensah JA, Osei-Safo D, Adjei S, Eklemet AA, et al. An extract of Synedrella nodiflora (L) Gaertn exhibits antidepressant properties through monoaminergic mechanisms. Metabolic Brain Disease 2018;33(4):1359-1368. doi: 10.1007/s11011-018-0244-0.
7.      Zuckerman H, Pan Z, Park C, Brietzke E, Musial N, Shariq AS, et al. Recognition and treatment of cognitive dysfunction in major depressive disorder. Frontiers in psychiatry. 2018;9:655. doi: 10.3389/fpsyt.2018.00655.
8.      Perez-Caballero L, Torres-Sanchez S, Bravo L, Mico JA, Berrocoso E. Fluoxetine: a case history of its discovery and preclinical development. Expert opinion on drug discovery 2014;9(5):567-78.doi: 10.1517/17460441.2014.907790.
9.     
34
 
Latendresse G, Elmore C, Deneris A. Selective Serotonin Reuptake Inhibitors as First-Line Antidepressant Therapy for Perinatal Depression. Journal of Midwifery & Women's Health 2017;62(3):317-28.doi: 10.1111/jmwh.12607.
10.  Rheker J, Winkler A, Doering BK, Rief W. Learning to experience side effects after antidepressant intakeResults from a randomized, controlled, double-blind study. Psychopharmacology 2017;234(3):329-38.doi: 10.1007/s00213-016-4466-8.
11.  Ranjbar M, Salehi A, Rezaeizadeh H, Zarshenas MM, Sadeghniiat-Haghighi K, Mirabzadeh M, et al. Efficacy of a Combination of Melissa officinalis L. and Nepeta menthoides Boiss. & Buhse on Insomnia: A Triple-Blind, Randomized Placebo-Controlled Clinical Trial. The Journal of Alternative and Complementary Medicine 2018;24(12):1197-203.doi: 10.1089/acm.2017.0153
12.  Kim N-S, Lee D-S. Comparison of different extraction methods for the analysis of fragrances from Lavandula species by gas chromatographymass spectrometry. Journal of Chromatography A 2002;982(1):31-47.doi: 10.1016/s0021-9673(02)01445-0.
13.  Abbasi Maleki S, Bekhradi R, Asgharpanah J, Abbasi Maleki F, Maleki Ahanghari N. Antidepressant-Like effect of aqueous and hydroalcoholic extracts of Lavandula angustifolia Mill in forced swim test and tail suspension test in male mice. Journal of Arak University of Medical Sciences 2013;16(9):65-75.
14. 
35
 
Chen SL, Chen CH. Effects of Lavender tea on fatigue, depression, and maternalinfant attachment in sleepdisturbed postnatal women. Worldviews on EvidenceBased Nursing 2015;12(6):370-9.doi: 10.1111/wvn.12122.
15.  Mahajan S, Engel WK. Assessment: symptomatic treatment for muscle cramps (an evidence-based review): report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurolog. Neurology 2010;75(15):1397-8. PMID: 20960612.
16.  Nikfarjam M, Rakhshan R, Ghaderi H. Comparison of Effect of Lavandula officinalis and Venlafaxine in Treating Depression: A Double Blind Clinical Trial. Journal of Clinical and Diagnostic Research 2017;11(7):KC01-KC4. doi: 10.7860/JCDR/2017/20657.10233.
17.  Park B-K, Kim YR, Kim YH, Yang C, Seo C-S, Jung IC, et al. Antidepressant-like effects of gyejibokryeong-hwan in a mouse model of reserpine-induced depression. Biomed Research International 2018;5845491. doi: 10.1155/2018/5845491.
18.  Saki K, Bahmani M, Rafieian-Kopaei M. The effect of most important medicinal plants on two importnt psychiatric disorders (anxiety and depression)-a review. Asian Pacific Journal of Tropical Medicine 2014;7:S34-S42.doi: 10.1016/S1995-7645(14)60201-7.
19.  Xian Y-F, Fan D, Ip S-P, Mao Q-Q, Lin Z-X. Antidepressant-like effect of isorhynchophylline in mice. Neurochemical Research 2017;42(2):678-685. doi: 10.1007/s11064-016-2124-5.
20.  Wang Y, Gao S-M, Li R, Zhang M, Gao S, Yu C-Q. Antidepressant-like effects of the Radix Bupleuri and Radix Paeoniae Alba drug pair. Neuroscience Letters 2016;633:14-20.doi: 10.1016/j.neulet.2016.09.001
21.  Seibenhener ML, Wooten MC. Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice. Journal of Visualized Experiments 2015(96):e52434.doi: 10.3791/52434.
22.  Page CE, Shepard R, Heslin K, Coutellier L. Prefrontal parvalbumin cells are sensitive to stress and mediate anxiety-related behaviors in female mice. Scientific Reports 2019;9(1):1-9.doi: 10.1038/s41598-019-56424-9.
23.  Kageyama A, Ueno T, Oshio M, Masuda H, Horiuchi H, Yokogoshi H. Antidepressant-like effects of an aqueous extract of lavender (Lavandula angustifolia Mill.) in rats. Food Science and Technology Research. 2012;18(3):473-9.doi.org/10.3136/fstr.18.473.
24.  Sarris J, Panossian A, Schweitzer I, Stough C, Scholey A. Herbal medicine for depression, anxiety and insomnia: a review of psychopharmacology and clinical evidence. European neuropsychopharmacology. 2011;21(12):841-60.doi: 10.1016/j.euroneuro.2011.04.002.
25. 
36
 
Abuhamdah S, Chazot PL. Lemon Balm and Lavender herbal essential oils: Old and new ways to treat emotional disorders?. Current Anaesthesia & Critical Care. 2008;19(4):221-6.doi.org/10.1016/j.cacc.2008.05.005.
26.  Akhondzadeh S, Kashani L, Fotouhi A, Jarvandi S, Mobaseri M, Moin M, et al. Comparison of Lavandula angustifolia Mill. tincture and imipramine in the treatment of mild to moderate depression: a double-blind, randomized trial. Progress in Neuro-Psychopharmacology and Biological Psychiatry 2003;27(1):123-7. doi: 10.1016/s0278-5846(02)00342-1.
27.  Koulivand PH, Khaleghi Ghadiri M, Gorji A. Lavender and the nervous system. Evidence-Based Complementary and Alternative Medicine. 2013;2013:681304. doi: 10.1155/2013/681304.
28.  Kyzar E, Stewart AM, Landsman S, Collins C, Gebhardt M, Robinson K, et al. Behavioral effects of bidirectional modulators of brain monoamines reserpine and d-amphetamine in zebrafish. Brain research. 2013;1527:108-16.doi: 10.1016/j.brainres.2013.06.033.
29.  Angrini M, Leslie JC, Shephard RA. Effects of propranolol, buspirone, pCPA, reserpine, and chlordiazepoxide on open-field behavior. Pharmacology Biochemistry and Behavior 1998;59(2):387-97.doi: 10.1016/s0091-3057(97)00457-7.