بررسی کارایی جداسازی و کشت سلول‌های بنیادی مزانشیمی پرده آمنیون انسان در محیط کشت سلولی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی واحد همدان

2 مرکز آموزشی- پژوهشی و درمانی سل و بیماری‌های ریوی بیمارستان مسیح دانشوری

3 دانشگاه علوم پزشکی تهران

10.22070/daneshmed.2021.12957.0

عنوان مقاله [English]

The Efficacy of isolation and culture of human amniotic mesenchymal stem cells in cell culture medium

نویسندگان [English]

  • Rahim Ahmadi 1
  • Sona Zare 1
  • Abdolreza Mohammadnia 2
  • Mohammad Ali Nilforouszadeh 3
  • Minoo Mahmoodi 1
چکیده [English]

Abstract

Background and Aim: The efficacy of methods for amniotic membrane derived mesenchymal stem cells isolation, culture and biological characterization face serious challenges. The aim of the present study is to investigate the efficacy of methods for amniotic membrane derived mesenchymal stem cells isolation, culture and differentiation in cell culture.
Materials and Methods: In this experimental laboratory study 10 placenta specimens were obtained from pregnant mothers during cesarean section and amniotic membranes were separated. The protocol for isolation and culture of mesenchymal cells was optimized by enzymatic method. The morphological characteristics of mesenchymal cells were examined by invert microscopy and biological characteristics were measured by flow cytometry and differentiation capacity was evaluated by mesenchymal cells capacity to differentiate to osteocyte and adipocyte. Data were analyzed using descriptive statistics.
Results: Expression level of CD44, CD73, CD90, and CD29 was significant in cells isolated form human amniotic cells membrane. CD14, CD34 and CD45 did not expressed or slightly expressed. The cells had high viability and successfully differentiated into osteocyte and adipocyte.
Conclusion: The protocol used in this study to isolate and culture human amniotic membrane derived mesenchymal stem cells was highly efficient to prepare mesenchymal stem cells for cell therapy and tissue engineering.

کلیدواژه‌ها [English]

  • Mesenchymal Stem Cells
  • Amniotic Membrane
  • Differentiation
  • Specific Markers