بررسی توانایی تولید بیوفیلم در سالمونلای جداشده از نمونه‌های گوشت مرغ بازارهای میوه و تره بار شهر تهران

نویسندگان

1 گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات میکروب‌شناسی مولکولی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

چکیده

مقدمه و هدف: یکی از موضوعات مهم در بهداشت و ایمنی مواد غذایی ظهور باکتری های مقاوم به آنتی‌بیوتیک است. در این میان عوامل بیماری‌زا و همچنین تشکیل بیوفیلم می‌توانند در افزایش مقاومت باکتری‌ها نقش داشته باشند. در این مطالعه، شکل‌گیری بیوفیلم در نمونه‌های سالمونلا جدا شده از مرغ، مقاومت دارویی و الگوی مقاومت دارویی در آنها مورد بررسی قرار گرفت.
مواد و روش ها: در این مطالعه ۷۵ نمونه گوشت مرغ آلوده به باکتری سالمونلا مورد بررسی قرار گرفت. میزان تشکیل بیوفیلم در آنها با روش میکرتیترپلیت بررسی شد. مقاومت دارویی و بررسی الگوی مقاومت دارویی در آنها نیز ارزیابی شد.
نتایج: نتایج نشان داد که در 92٪ (75/69) از نمونه‌های سالمونلا بیوفیلم تشکیل شد که در میان آن بیوفیلم متوسط با 65.2% بیشترین میزان تولید بیوفیلم را داشت. مقاومت همزمان به 2 کلاس آنتی‌بیوتیک‌ها (2DR) با 31.8% (69/22) شایع‌ترین نوع مقاومت چند دارویی (MDR) در بین جدایه های تشکیل دهنده بیوفیلم بود. مقاومت به ایمی پنم، سفتریاکسون و سفوتاکسیم تنها در 1 نمونه تشکیل دهنده بیوفیلم مشاهده شد.
نتیجه‌گیری: در این مطالعه میزان بالایی از تشکیل بیوفیلم در نمونه‌های گوشت مرغ آلوده به سالمونلا مشاهده شد. میزان بالای مقاومت آنتی‌بیوتیکی در نمونه‌های تشکیل‌دهنده بیوفیلم رعایت هر چه بیشتر موارد بهداشتی در عدم انتقال آلودگی مواد غذایی به انسان را مورد توجه قرار می دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of biofilm production capacity in salmonella isolated from chicken meat in Tehran municipally daily fruit and vegetable markets

نویسندگان [English]

  • Saeid Besharati 1
  • Parviz Owlia 2
چکیده [English]

Background and Objective: One of the major issues in food hygiene and safety is the emergence of antibiotic-resistant bacteria. Pathogens as well as biofilm formation can play a role in increasing bacterial resistance. In this study, biofilm formation in Salmonella isolates from chicken, drug resistance and drug resistance pattern were investigated.
Materials and Methods: In this study, 75 samples of chicken infected with Salmonella bacteria were studied. The rate of biofilm formation was assessed by microtiter plate method. Drug resistance and evaluation of drug resistance pattern were also evaluated.
Results: The results showed that in 92% (69/75) of the samples Salmonella biofilm were formed, among which the moderate biofilm with 65.2% had the highest biofilm production. Concurrent resistance to 2 classes of antibiotics (2DR) with 31.8% (22/69) was the most common type of multidrug resistance (MDR) among the biofilm-forming isolates. Resistance to imipenem, ceftriaxone and cefotaxime was observed in only 1 biofilm formation sample.
Conclusion: In this study, high biofilm formation was observed in Salmonella contaminated chicken meat samples. The high rate of antibiotic resistance in biofilm constituents is due to the increasing attention to hygienic aspects of non-transmission of food contamination to humans.

کلیدواژه‌ها [English]

  • Salmonella
  • Biofilm
  • Antibiotic resistance
  • Chicken meat
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